A berberrubine-derived fluorescent probe for hydrazine and its practical application in water and food samples

A Ratiometric Fluorescent Probe for N2H4 Having a Large Detection Range Based upon Coumarin with Multiple Applications

“…Initially, we cultured MC3T3-E1 cells in 96-well plates for 1 day and allowed them to adhere. Different concentrations of CHAC (10,20,30,40, and 50 µM) were then added and incubated for another 1 day. The culture media in the wells were removed and an MTT solution (5 mg/mL, 20 µL) was added to every well.…”

“…Fluorescence-based methods, meanwhile, have been broadly applied to verify different analytes, including metal ions, anions, and biomolecules, because of their simple operation, high sensitivity, good selectivity, non-invasiveness, and good biocompatibility [15,16]. Several selective N 2 H 4 fluorescent probes (FPs) with various recognition mechanisms [17,18], including selective deprotection [19][20][21][22]; reaction with diketones to form rings [23,24]; reactions with aromatic aldehydes, dicyanovinyl, or monocyanovinyl to form hydrazones [25][26][27][28][29]; cleavage of carbon-carbon double bonds Molecules 2023, 28, 7629 2 of 13 to form hydrazones [30,31]; and hydrazine-induced ring-opening reactions [32,33] have been reported. However, many of these probes still possess disadvantages, such as their complex synthesis, narrow pH range, low sensitivity, and small detection range, which have limited their application.…”

“…(FPs) with various recognition mechanisms [17,18], including selective deprotection [19][20][21][22]; reaction with diketones to form rings [23,24]; reactions with aromatic aldehydes, dicyanovinyl, or monocyanovinyl to form hydrazones [25][26][27][28][29]; cleavage of carbon-carbon double bonds to form hydrazones [30,31]; and hydrazine-induced ring-opening reactions [32,33] have been reported. However, many of these probes still possess disadvantages, such as their complex synthesis, narrow pH range, low sensitivity, and small detection range, which have limited their application.…”

A thiomorpholine substituted malonyl-coumarin dye for discriminative detection of hydrazine and strong acidity

“…[7][8][9][10] Their rapid development could be due to the advantages including good selectivity, sensitivity, low cost, easy operation and on-site monitoring, etc. 11,12 To detect N 2 H 4 , uorescent probes have been designed based on different strategies including hydroxyl/amino protectiondeprotection, [13][14][15][16] group transformation, [17][18][19][20][21][22] cyclization, 23,24 lactonic ring-opening 25 and regulation of hydrogen bonds. 26,27 To detect acidity, probes have been designed mainly based on the acid-base neutralization (for some probes which work in alkaline conditions, nucleophilic addition reaction of the hydroxyl ion was involved).…”