A Battery-Powered Notebook Thermal Cycler for Rapid Multiplex Real-Time PCR Analysis

Belgrader, Phillip; Young, Steve; Yuan, Bob; Primeau, Michael; Christel, L. A.; Pourahmadi, and Farzad; Northrup, M. Allen

doi:10.1021/ac000905v

Cited by 102 publications

(60 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tests could be completed in as little as 16 min, with detection limits on the order of 10 2 -10 4 organisms/mL. A portable instrument incorporating a four-color fluorescence detection system was developed by the same group for rapid, multiplex real-time PCR [224]. Use of this instrument for rapid thermal cycling in a 25 mL volume allowed the characterization of a SNP in the hereditary hemochromatosis gene in about 20 min.…”

Section: Clinical Dna By Microchip Pcrmentioning

confidence: 99%

Microfluidic chips for clinical and forensic analysis

2002

View full text Add to dashboard Cite

This review gives an overview of developments in the field of microchip analysis for clinical diagnostic and forensic applications. The approach chosen to review the literature is different from that in most microchip reviews to date, in that the information is presented in terms of analytes tested rather than microchip method. Analyte categories for which examples are presented include (i) drugs (quality control, seizures) and explosives residues, (ii) drugs and endogenous small molecules and ions in biofluids, (iii) proteins and peptides, and (iv) analysis of nucleic acids and oligonucleotides. Few cases of microchip analysis of physiological samples or other "real-world" matrices were found. However, many of the examples presented have potential application for these samples, especially with ongoing parallel developments involving integration of sample pretreatment onto chips and the use of fluid propulsion mechanisms other than electrokinetic pumping.

show abstract

Section: Clinical Dna By Microchip Pcrmentioning

confidence: 99%

Microfluidic chips for clinical and forensic analysis

2002

View full text Add to dashboard Cite

show abstract

“…[21] In 2001, a batterypowered notebook PCR instrument that performed rapid PCR analysis was developed. [22] Through independently controlled thermal cycler modules, rapid detection and identification of Bacillus subtilis and Bacillus thuringensis was observed. [22] The Handheld Advanced Nucleic Acid Analyzer (HANAA) is a battery-powered thermal cycler that utilizes silicon and platinum-based thermocycler units to perform four real time PCR reactions simultaneously.…”

Section: Pcr Amplificationmentioning

confidence: 99%

“…[22] Through independently controlled thermal cycler modules, rapid detection and identification of Bacillus subtilis and Bacillus thuringensis was observed. [22] The Handheld Advanced Nucleic Acid Analyzer (HANAA) is a battery-powered thermal cycler that utilizes silicon and platinum-based thermocycler units to perform four real time PCR reactions simultaneously. [19] HANAA can detect a variety of bacterial organisms via real time PCR.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Flow through PCR module of BioBriefcase

et al. 2005

View full text Add to dashboard Cite

The BioBriefcase is an integrated briefcase-sized aerosol collection and analysis system for autonomous monitoring of the environment, which is currently being jointly developed by Lawrence Livermore and Sandia National Laboratories. This poster presents results from the polymerase chain reaction (PCR) module of the system. The DNA must be purified after exiting the aerosol collector to prevent inhibition of the enzymatic reaction. Traditional solid-phase extraction results in a large loss of sample. In this flow-through system, we perform sample purification, concentration and amplification in one reactor, which minimizes the loss of material. The sample from the aerosol collector is mixed with a denaturation solution prior to flowing through a capillary packed with silica beads. The DNA adheres to the silica beads allowing the environmental contaminants to be flushed to waste while effectively concentrating the DNA on the silica matrix. The adhered DNA is amplified while on the surface of the silica beads, resulting in a lower limit of detection than an equivalent eluted sample. Thus, this system is beneficial since more DNA is available for amplification, less reagents are utilized, and contamination risks are reduced.

show abstract

“…In this frame, since the first PCR was carried out on a chip (Kopp et al 1998), a large proliferation of biodevices with different geometries (single-or multiwell based) (Northrup et al 1998;Belgrader et al 2001;Neuzil et al 2006) and/or equipped with microfluidic systems (Shaikh et al 2005) have been reported. Several materials (e.g., silicon (Weiping et al 2005), quartz (Jacobson et al 1995), glass (Giordano et al 2001), ITO (Suna et al 2002), etc.)…”

Section: Introductionmentioning

confidence: 99%

Disposable plastic microreactors for genomic analyses

Sabella

Vecchio

Pompa

et al. 2009

Biomed Microdevices

View full text Add to dashboard Cite

We show the design, development and assessment of disposable, biocompatible, fully plastic microreactors, which are demonstrated to be highly efficient for genomic analyses, such as amplification of DNA, quantitative analyses in real time, multiplex PCR (both in terms of efficiency and selectivity), as compared to conventional laboratory equipment for PCR. The plastic microreactors can easily be coupled to reusable hardware, enabling heating/cooling processes and, in the case of qPCR applications, the real-time detection of the signal from a suitable fluorescent reporter present in the reaction mixture during the analysis. The low cost production of these polymeric microreactors, along with their applicability to a wide range of biochemical targets, may open new perspectives towards practical applications of biochips for point of care diagnostics.

show abstract

A Battery-Powered Notebook Thermal Cycler for Rapid Multiplex Real-Time PCR Analysis

Cited by 102 publications

References 21 publications

Microfluidic chips for clinical and forensic analysis

Microfluidic chips for clinical and forensic analysis

Flow through PCR module of BioBriefcase

Disposable plastic microreactors for genomic analyses

Contact Info

Product

Resources

About