A battery of translational biomarkers for the assessment of the in vitro and in vivo antioxidant action of plant polyphenolic compounds: The biomarker issue

Veskoukis, Aristidis S.; Kerasioti, Efthalia; Priftis, Alexandros; Kouka, Paraskevi; Spanidis, Ypatios; Makri, Sotiria; Kouretas, Demetrios

doi:10.1016/j.cotox.2018.10.001

Cited by 59 publications

(73 citation statements)

References 75 publications

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“…Three redox biomarkers, namely total antioxidant capacity (TAC) as a crude index of the antioxidant potential of the samples, thiobarbituric acid reactive substances (TBARS) as a biomarker of lipid peroxidation, and reduced glutathione (GSH) as the most important intrinsic antioxidant molecule, were evaluated in all blood samples [27]. TAC was measured on the basis of the protocol of Janaszewska and Bartosz [28] as previously described [29].…”

Section: Laboratory Examinationsmentioning

confidence: 99%

Effects of Melatonin Administration to Pregnant Ewes under Heat-Stress Conditions, in Redox Status and Reproductive Outcome

et al. 2020

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Heat stress is a known promoter of reactive oxygen species generation, which may compromise pregnancy and foetal development. Melatonin is a pleiotropic molecule that regulates various processes including pregnancy. Thus, it could be used to ameliorate the redox status of pregnant heat-stressed ewes and the outcome of their pregnancy. Sixty-eight ewes participated in the study, which were allocated into two equal groups, i.e., Melatonin (M) and Control (C) group. All ewes were exposed to heat stress from D0 to D120. In both groups, after oestrus synchronization of ewes, rams were introduced to them for mating (D16). In M group, starting with sponges’ insertion (D0), melatonin implants were administered four-fold every 40 days. Pregnancy diagnosis was performed by means of ultrasonography. Daily evaluation of temperature humidity index (THI), rectal temperature, and breathing rate were performed throughout the study. Blood samples were collected repeatedly from D0 until weaning for assaying redox biomarkers. Milk yield was measured thrice during puerperium. The results showed that melatonin administration throughout pregnancy improved the redox status of heat-stressed ewes and increased the mean number and bodyweight of lambs born per ewe, as well as the milk production. Therefore, melatonin may be used as antioxidant regimen in heat-stressed ewes for improving their reproductive traits.

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Section: Laboratory Examinationsmentioning

confidence: 99%

Effects of Melatonin Administration to Pregnant Ewes under Heat-Stress Conditions, in Redox Status and Reproductive Outcome

et al. 2020

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“…Determination of glutathione S-transferase (GST) activity. GST activity in the tissue homogenate was measured according to the method of Habig et al (1974) (43) as described previously (7,11,44). Briefly, the appropriate amount of potassium phosphate buffer (K 2 HPO 4 and KH 2 PO 4 , 100 mM, pH=7.4) was mixed with 50 μl of 1 mM GSH solution and 20 μl of 1 mM 1-chloro-2,4-dinitrobenzene (CDNB, Sigma-Aldrich) diluted in 95% ethanol and the samples were incubated at 37˚C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Biofunctional Feed Supplemented With By-products of Olive Oil Production Improves Tissue Antioxidant Profile of Lambs

Makri

Raftopoulou

Kafantaris

et al. 2020

In Vivo

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Background/Aim: Olive mill wastewater (OMW) is a byproduct of olive oil production. The aim of the study was to estimate the redox profile of lambs' vital organs after consumption of an OMW-supplemented feed. Materials and Methods: Twenty-four lambs received breast milk until day 15. Then, they were divided in two groups: control and OMW, n=12 each. The control group received standard ration, while the OMW group received OMW enriched feed along with mother's milk until day 42 and animals (n=6 per group) were sacrificed. The remaining 12 received the feeds until day 70 and sacrificed. Tissue samples were collected at day 42 and 70 and specific redox biomarkers were assessed. Results: Overall, the OMW feed improved tissue redox profile by affecting the glutathione S-transferase (GST) activity and γ-glutamate-cysteine ligase (γ-GCL) expression in all tested tissues. Superoxide dismutase (SOD) activity was not affected. Conclusion: The polyphenol-rich byproduct reinforced lamb redox profile and may putatively improve their wellness and productivity. Olive oil is an extract derived from olives and it has been mostly used in the Mediterranean basin since the 8 th millennium BC (1). Olive oil production rates vary between countries, such as Spain, Italy, Tunisia and Portugal, which according to the Food and Agriculture Organization of the United Nations are among the leading producers of olive oil worldwide (FAOSTAT). According to the same source, Greece is responsible for an average annual production of 344,615 tons of olive oil from 1993 to 2014 being, thus, third in the ranking of olive oil producer countries. On the basis of the Integrated Pollution Prevention and Control (IPPC) Directive, European industries produce 72% of the olive oil produced annually worldwide (Spain: 45%, Italy: 15%, Greece: 10%) (2). The extraction and production of olive oil in Greece is carried out in approximately 2,800 small-scale agro-industrial units scattered throughout the country (3, 4). Although olive oil production is a fundamental procedure for the economy of every country, it is known that it can promote environmental pollution due to the generation of two waste streams, namely olive mill residual solids and olive mill wastewater (OMW) (3). OMW is mainly characterized not only by high organic content and complex organic substances but also by the presence of diverse phenolic compounds (5). These compounds are monocyclic and polymeric aromatic molecules and are responsible for the dark color, the phytotoxicity, the antibacterial properties and the unpleasant odor of OMW (3, 5, 6). OMW has an acidic pH, ranging between 4 and 5, and mostly consists of polyphenols and tannins, while the 2-15% of its organic fraction is also rich in phenolic compounds with a concentration range between 3 and 10 g/l (3, 5). Olive mill residues are reach in polyphenolic compounds, such as caffeic acid, coumaric acid, ferulic acid, gallic acid, hydroxybenzoic acid, kaempherol and quercetin. However, oleuropein, tyrosol and hydroxytyrosol are the most cru...

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“…In particular, the pathogenesis of common diseases, such as pneumonia (9), enteritis (10) and sepsis (11,12) is associated with disorders in animal redox status and, more specifically, with oxidative modifications leading to the damage of biomolecules (i.e., proteins, lipids and DNA). The oxidation of macromolecules generates various end-products that can be measured and characterized as redox biomarkers in order to assess oxidative stress in vivo (7). Towards this direction, in order to clarify the impact of oxidative stress on farm animal welfare, a wide range of biomarkers in biological fluids and tissues has been adopted.…”

Section: Regulation Of Redox Status and Farm Animal Welfarementioning

confidence: 99%

“…Specifically, recent research focuses on the administration of natural antioxidants to livestock in order to enhance the health and productivity of farm animals, mainly by protecting cellular macromolecules from detrimental oxidative modifications (1)(2)(3)(4)(5)(6). Therefore, a specific need for a battery of efficient biomarkers that reflect the extend of biomolecule oxidative damage has emerged (7). The adoption of redox biomarkers, which depict the action of the antioxidant defense system and the harmful role of reactive species on biomolecules that are changed by redox altering stimuli, will enable researchers to introduce nutritional practices with beneficial antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

Farm animal welfare, productivity and meat quality: Interrelation with redox status regulation and antioxidant supplementation as a nutritional intervention (Review)

Veskoukis

Kouretas

2019

Wrld Acd Sci

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Contemporary concerns over farm animal welfare have transpired as a result of the desire of farmers to gain the benefits of the increased productivity of their farming animals. Inspired by the constant flow of scientific knowledge, technology and new production methods, the progressive exploitation of the animal biological capacity to produce economic output, has raised increasing concerns as to the extent to which the animals are being stressed. In fact, individuals worldwide depend heavily on animals for the production of meat, fat, milk and other essential products. However, in recent years, the demand for livestock products has increased tremendously and world livestock production is facing major challenges, which endanger the health of the animals. Furthermore, the utilization of proper antioxidant supplementation as an important and cost-effective treatment for beneficial effects on farm animals is also a major pillar for the manipulation of current needs in modern animal husbandry. In this comprehensive review, we aimed to summarize the knowledge regarding the interrelation of farm animal welfare, productivity and meat quality with redox status regulation and antioxidant supplementation as a promising nutritional intervention. We propose that evaluating various biomarkers may help researchers to adopt the optimal growth conditions of farm animals, a fact that is expected to protect them from redox-related pathologies, in order to enhance their productivity and to strengthen the quality of their products. Contents 1. Introduction 2. Regulation of redox status and farm animal welfare 3. Regulation of redox status and farm animal productivity 4. Regulation of redox status and farm animal meat quality 5. Conclusions

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A battery of translational biomarkers for the assessment of the in vitro and in vivo antioxidant action of plant polyphenolic compounds: The biomarker issue

Cited by 59 publications

References 75 publications

Effects of Melatonin Administration to Pregnant Ewes under Heat-Stress Conditions, in Redox Status and Reproductive Outcome

Effects of Melatonin Administration to Pregnant Ewes under Heat-Stress Conditions, in Redox Status and Reproductive Outcome

Biofunctional Feed Supplemented With By-products of Olive Oil Production Improves Tissue Antioxidant Profile of Lambs

Farm animal welfare, productivity and meat quality: Interrelation with redox status regulation and antioxidant supplementation as a nutritional intervention (Review)

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