A Basis of the Stomatocytoses of Erythrocytes by 1-Chloro-2, 4-Dinitrobenzene and Other Electrophilic Reagents

Abstract

On bases of previous observations on the band 3 anion exchange inhibition by the electrophilic reagent 1-fluoro-2,4-dinitrobenzene in resealed ghosts and a previously proposed band 3-based mechanism of control of the erythrocyte shape, it is suggested that stomatocytoses by 1-chloro-2,4-dinitrobenzene and other electrophilic reagents are due to an inhibition of the band 3 anion exchange by a covalent modification of Lys 539, one of the two lysine intramolecularly cross-linked by impermeant 4,4'-diisothiocyanodihydrostilbene-2,2'-disulfonic acid, a band 3 anion exchange potent inhibitor. Attempts to explain these stomatocytoses led to explain the echinocytosis and competitive inhibition of band 3 anion exchange by stilbenedisulfonic acid derivatives and the reversal of the echinocytosis by 2,4-dinitrophenol by one stomatocytogenic of the electrophilic reagents. The inference on stomatocytoses by electrophilic reagents is of interest since stomatocytogenic and echinocytogenic by amphiphiles are perceived to interact noncovalently with the membrane and that they can be clinically relevant.