A barcode for the authentication of the snappers (Lutjanidae) of the western Atlantic: rDNA 5S or mitochondrial COI?

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR

“…The COI gene is a highly effective and reliable tool for the diagnosis of the authenticity of fishery products (Veneza et al, 2014), and the multiplex PCR technique has proven especially effective for the detection of endangered species De-Franco et al, 2010;Alexandre de-Franco et al, 2012). In this case, the low cost of the synthesis of the specific primers for the epinephelid species developed in the present study, and the simplicity of the multiplex PCR technique may favor the adoption of this more effective method for forensic identification, with the added advantage of providing more precise statistics on the fishery exploitation of the epinephelids along the Brazilian coast.…”

“…Genetic tools for the molecular identification of a number of different fish species have been developed during the past decade (Ward et al, 2005;Craig et al, 2009;Hashimoto et al, 2010;Rodrigues et al, 2011;Alexandre de-Franco et al, 2012;Ulrich et al, 2013;Veneza et al, 2014). Carvalho et al (2015) has shown the DNA barcoding to be an effective molecular tool for the detection of commercially-valuable species such as the groupers, while Torres et al (2013) successfully applied PCR RFLP and SNP detection techniques to the identification of specimens of E. itajara, E. morio, M. bonaci, and M. marginata.…”

Towards more sustainable surimi? PCR-cloning approach for DNA barcoding reveals the use of species of low trophic level and aquaculture in Asian surimi

“…Rasmussen & Morrissey, 2011). DNA barcoding has proven highly effective in different genetic authentication studies, from invertebrates to vertebrates, detecting species substitution for a variety of taxa (Galal-Khallaf, Ardura, Mohammed-Geba, Borrell, & Garcia-Vazquez, 2014;Haye, Segovia, Vera, Gallardo, & Gallardo-Esc arate, 2012;Maralit et al, 2013;Veneza et al, 2014;Yang, Huang, Hsieh, Huang, & Chen, 2012). Among the most popular markers are a 650 base pair (bp) fragment of the Cytochrome oxidase subunit I (COI) gene and partial fragment of 16S rDNA that have been successfully applied for many species identification studies (Cawthorn, Steinman, & Witthuhn, 2012;Hebert, Ratnasingham, & deWaard, 2003).…”

DNA barcoding reveals high substitution rate and mislabeling in croaker fillets (Sciaenidae) marketed in Brazil: The case of “pescada branca” (Cynoscion leiarchus and Plagioscion squamosissimus)

“…The COI has also been successfully used in the identification of processed fish, elucidating various substitutions and commercial fraud cases (Barbuto et al, 2010;Carvalho et al, 2011a;Cawthorn et al, 2012;Galimberti et al, 2013;Hanner, Becker, Ivanova, & Steinke, 2011;Huxley-Jones et al, 2012). In Brazil, few studies have applied the barcoding technology for identifying fish (Carvalho et al, 2011a;Pereira, Hanner, Foresti, & Oliveira, 2013;Pereira, Maia, Hanner, Foresti, & Oliveira, 2011a;Pereira et al, 2011b;Ribeiro et al, 2012;Veneza et al, 2014) or for assessing species substitution and mislabeling (Ardura et al, 2010;Carvalho et al, 2011b).…”