A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO2

Ku, Jason T.; Lan, Ethan I.

doi:10.1016/j.ymben.2018.02.004

Cited by 38 publications

(15 citation statements)

References 46 publications

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“…Acetate concentration reached 37 mg/L and crotonate reached 540 μg/L. Previous study on Synechocystis PCC 6803 producing 3‐hydroxybutyrate (Angermayr et al, ; Ku & Lan, ; Lan et al, ; Varman et al, ; B. Wang et al, ), using the same CoA pathway, has shown that biosynthesis of products from the CoA pathway occurs during low nitrogen availability, consistent with production of poly‐3‐hydroxybutyrate as carbon storage. In this study, we measured the nitrate concentration in our culture medium (Supporting Information Figure S2) and observed no significant correlation between productivity and nitrogen availability.…”

Section: Resultsmentioning

confidence: 58%

“…Among photosynthetic microbes, cyanobacteria are photoautotrophic prokaryotes that have simpler and more developed genetic tools than microalgae. As such, they have attracted significant attention as the potential next generation cell factory and have been used to produce various chemicals including alcohols (Atsumi, Higashide, & Liao, ; Z. X. Gao, Zhao, Li, Tan, & Lu, ; Hirokawa, Dempo, Fukusaki, & Hanai, ), hydroxyacids (Angermayr et al, ; Ku & Lan, ; Lan et al, ; Varman, Yu, You, & Tang, ; B. Wang, Pugh, Nielsen, Zhang, & Meldrum, ; Y. Wang et al, ), diacids (Chin, Sano, Takahashi, Ohara, & Aso, ; Lan & Wei, ), olefins (Bentley, García‐Cerdán, Chen, & Melis, ; Formighieri & Melis, ; X. Gao et al, ; Xiong et al, ), diols (Hirokawa, Maki, Tatsuke, & Hanai, ; Oliver, Machado, Yoneda, & Atsumi, ), and others (Angermayr, Gorchs Rovira, & Hellingwerf, ; Case & Atsumi, ; Lai & Lan, ). However, to our knowledge, direct photosynthetic butyrate production has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Photoautotrophic synthesis of butyrate by metabolically engineered cyanobacteria

Lai

Lan

2019

Biotech & Bioengineering

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Direct conversion of carbon dioxide into chemicals using engineered autotrophic microorganisms offers a potential solution for both sustainability and carbon mitigation.Butyrate is an important chemical used in various industries, including fragrance, food, and plastics. A model cyanobacterium Synechococcus elongatus PCC 7942 was engineered for the direct photosynthetic conversion of CO 2 to butyrate. An engineered Clostridium Coenzyme A (CoA)-dependent pathway leading to the synthesis of butyryl-CoA, the precursor to butyrate, was introduced into S. elongatus PCC 7942. Two CoA removal strategies were then individually coupled to the modified CoA-dependent pathway to yield butyrate production. Similar results were observed between the two CoA removal strategies. The best butyrate producing strain of S. elongatus resulted in an observed butyrate titer of 750 mg/L and a cumulative titer of 1.1 g/L. These results demonstrated the feasibility of photosynthetic butyrate production and expanded the chemical repertoire accessible for production by photoautotrophs. K E Y W O R D Sbutyrate kinase, butyric acid, cyanobacteria, metabolic engineering, phosphotransbutyrylase, thioesterase

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Section: Resultsmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Photoautotrophic synthesis of butyrate by metabolically engineered cyanobacteria

Lai

Lan

2019

Biotech & Bioengineering

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“…It has been proved that providing extra ATP by regulating CEF as the main electron transport chain inhibits the process of carbon fixation [ 127 ]. Ku et al [ 128 ] engineered an ATP-hydrolysis based driving force module into Synechococcus elongatus PCC 7942 to produce 3-hydroxybutyrate at the expense of cell growth. Selecting light to highlight CEF also shows weak enhanced effect in overall process of microalgae cultivation [ 129 ].…”

Section: Key Engineering Strategiesmentioning

confidence: 99%

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion

Sun

Zhao

Mao

et al. 2018

Biotechnol Biofuels

106

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Microalgae are capable of producing sustainable bioproducts and biofuels by using carbon dioxide or other carbon substances in various cultivation modes. It is of great significance to exploit microalgae for the economical viability of biofuels and the revenues from high-value bioproducts. However, the industrial performance of microalgae is still challenged with potential conflict between cost of microalgae cultivation and revenues from them, which is mainly ascribed to the lack of comprehensive understanding of carbon metabolism and energy conversion. In this review, we provide an overview of the recent advances in carbon and energy fluxes of light-dependent reaction, Calvin–Benson–Bassham cycle, tricarboxylic acid cycle, glycolysis pathway and processes of product biosynthesis in microalgae, with focus on the increased photosynthetic and carbon efficiencies. Recent strategies for the enhanced production of bioproducts and biofuels from microalgae are discussed in detail. Approaches to alter microbial physiology by controlling light, nutrient and other environmental conditions have the advantages of increasing biomass concentration and product yield through the efficient carbon conversion. Engineering strategies by regulating carbon partitioning and energy route are capable of improving the efficiencies of photosynthesis and carbon conversion, which consequently realize high-value biomass. The coordination of carbon and energy fluxes is emerging as the potential strategy to increase efficiency of carbon fixation and product biosynthesis. To achieve more desirable high-value products, coordination of multi-stage cultivation with engineering and stress-based strategies occupies significant positions in a long term.

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“…Microalgae also serve as potential expression systems for the synthesis of biopolymers such as poly-3-hydroxybutyrate, which is a key precursor for the synthesis of biodegradable plastics. In a recent study, an ATP hydrolysis-based driving force module was engineered into Synechococcus elongatus PCC 7942 to produce 3-hydroxybutyrate [ 48 ]. The strain which was engineered by having a provision for a reversible outlet for excessive carbon flux was capable of producing significantly high amounts of 3-hydroxybutyrate over the native strain.…”

Section: Products From Algal Biorefinerymentioning

confidence: 99%

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Jagadevan

Banerjee

et al. 2018

Biotechnol Biofuels

203

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In the wake of the uprising global energy crisis, microalgae have emerged as an alternate feedstock for biofuel production. In addition, microalgae bear immense potential as bio-cell factories in terms of producing key chemicals, recombinant proteins, enzymes, lipid, hydrogen and alcohol. Abstraction of such high-value products (algal biorefinery approach) facilitates to make microalgae-based renewable energy an economically viable option. Synthetic biology is an emerging field that harmoniously blends science and engineering to help design and construct novel biological systems, with an aim to achieve rationally formulated objectives. However, resources and tools used for such nuclear manipulation, construction of synthetic gene network and genome-scale reconstruction of microalgae are limited. Herein, we present recent developments in the upcoming field of microalgae employed as a model system for synthetic biology applications and highlight the importance of genome-scale reconstruction models and kinetic models, to maximize the metabolic output by understanding the intricacies of algal growth. This review also examines the role played by microalgae as biorefineries, microalgal culture conditions and various operating parameters that need to be optimized to yield biofuel that can be economically competitive with fossil fuels.

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A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO2

Cited by 38 publications

References 46 publications

Photoautotrophic synthesis of butyrate by metabolically engineered cyanobacteria

Photoautotrophic synthesis of butyrate by metabolically engineered cyanobacteria

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

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