A bacteriophage mimic of the bacterial nucleoid-associated protein Fis

Chakraborti, Soumyananda; Balakrishnan, Dhanasekaran; Trotter, Alexander J.; Gittens, William; Yang, Ally; Jolma, Arttu; Paterson, Joy R; Swiatek, S.; Plewka, Jacek; Curtis, Fiona A.; Bowers, Laura Y.; Pålsson, Lars‐Olof; Hughes, Timothy R.; Taube, Michał; Kozak, Maciej; Heddle, Jonathan G.; Sharples, Gary J.

doi:10.1042/bcj20200146

Cited by 2 publications

(1 citation statement)

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“…HNH endonucleases have also been shown to act as mobile components that are deployed through horizontal transfer and genetic exchange [ 38 ], along with Gp77, which shows similarity to the NinH protein, which affects both cellular and viral DNA processing as well as gene expression, with advantageous effects on phage multiplication and dissemination [ 9 ]. The NinH-encoding gene is located at the end of the phage genome, at the same location where the 97% identical NinH-encoding gene of Raoultella phage RP180 is found.…”

Section: Resultsmentioning

confidence: 99%

Characterization and genome analysis of Escherichia phage fBC-Eco01, isolated from wastewater in Tunisia

et al. 2023

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The rise of antibiotic resistance in bacterial strains has led to vigorous exploration for alternative treatments. To this end, phage therapy has been revisited, and it is gaining increasing attention, as it may represent an efficient alternative for treating multiresistant pathogenic bacteria. Phage therapy is considered safe, and phages do not infect eukaryotic cells. There have been many studies investigating phage-host bacteria interactions and the ability of phages to target specific hosts. Escherichia coli is the causative agent of a multitude of infections, ranging from urinary tract infections to sepsis, with growing antibiotic resistance. In this study, we characterized the Escherichia phage fBC-Eco01, which was isolated from a water sample collected at Oued, Tunis. Electron microscopy showed that fBC-Eco01 phage particles have siphovirus morphology, with an icosahedral head of 61 ± 3 nm in diameter and a non-contractile tail of 94 ± 2 nm in length and 12 ± 0.9 nm in width. The genome of fBC-Eco01 is a linear double-stranded DNA of 43.466 bp with a GC content of 50.4%. Comparison to databases allowed annotation of the functions to 39 of the 78 predicted gene products. A single-step growth curve revealed that fBC-Eco01 has a latent period of 30 minutes and a burst size of 175 plaque-forming units (PFU) per infected cell. Genomic analysis indicated that fBC-Eco01 is a member of the subfamily Guernseyvirinae. It is most closely related to a group of phages of the genus Kagunavirus that infect Enterobacter, Raoultella, and Escherichia strains.

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Section: Resultsmentioning

confidence: 99%