A 90-Day Safety Study of Meat from MSTN and FGF5 Double-Knockout Sheep in Wistar Rats

Zhao, Yue; Chen, Mingming; Yao, Li; Xu, Xueling; Wu, S. Vincent; Liu, Zhimei; Qi, Shaohua; Yi, Guang; Zhang, Xiaosheng; Zhang, Jinlong; Guo, Xiaofei; Yu, Kun; Deng, Shoulong; Li, Yan; Lian, Zhengxing

doi:10.3390/life12020204

Cited by 4 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…116 After performing routine urine and blood tests, examining blood biochemical indicators, weight, and histopathologically examining major organs, it was shown that all the investigated values were within the normal ranges, and consuming mutton from MSTN-edited sheep, with concentrations of 3.75%, 7.5%, and 15%, did not have any long-term adverse or toxic effects on rat health. 116 These results support the potential of meat from MSTN-edited sheep for future human consumption.…”

Section: ■ Targeting the Mstn Gene In Sheepmentioning

confidence: 93%

“…The trio-based sequencing approach revealed a low incidence of SNVs and Indels and almost negligible off-target modifications. Furthermore, a 90-day feeding study for the safety evaluation of meat from MSTN - FGF5 dual knockout sheep in Wistar rats was performed . After performing routine urine and blood tests, examining blood biochemical indicators, weight, and histopathologically examining major organs, it was shown that all the investigated values were within the normal ranges, and consuming mutton from MSTN -edited sheep, with concentrations of 3.75%, 7.5%, and 15%, did not have any long-term adverse or toxic effects on rat health .…”

Section: Targeting the Mstn Gene In Sheepmentioning

confidence: 99%

See 1 more Smart Citation

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Kalds

Zhou

Huang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

How to increase meat production is one of the main questions in animal breeding. Selection for improved body weight has been made and, due to recent genomic advances, naturally occurring variants that are responsible for controlling economically relevant phenotypes have been revealed. The myostatin (MSTN) gene, a superstar gene in animal breeding, was discovered as a negative controller of muscle mass. In some livestock species, natural mutations in the MSTN gene could generate the agriculturally desirable double-muscling phenotype. However, some other livestock species or breeds lack these desirable variants. Genetic modification, particularly gene editing, offers an unprecedented opportunity to induce or mimic naturally occurring mutations in livestock genomes. To date, various MSTN-edited livestock species have been generated using different gene modification tools. These MSTN gene-edited models have higher growth rates and increased muscle mass, suggesting the high potential of utilizing MSTN gene editing in animal breeding. Additionally, post-editing investigations in most livestock species support the favorable influence of targeting the MSTN gene on meat quantity and quality. In this Review, we provide a collective discussion on targeting the MSTN gene in livestock to further encourage its utilization opportunities. It is expected that, shortly, MSTN gene-edited livestock will be commercialized, and MSTN-edited meat will be on the tables of ordinary customers.

show abstract

Section: ■ Targeting the Mstn Gene In Sheepmentioning

confidence: 93%

Section: Targeting the Mstn Gene In Sheepmentioning

confidence: 99%

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Kalds

Zhou

Huang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Previously, we prepared a MSTN and FGF5 double-knockout sheep by injecting Cas9 mRNA and sgRNA into zygotes, generating a MSTN and FGF5 dual-gene biallelic homozygous (MF −/− ) mutant (18). Here, we evaluated the reproductive capacity of MF −/− rams.…”

Section: Mstn and Fgf Double-knockout Does Not A Ect Semen Quality An...mentioning

confidence: 99%

Reproduction and viscera organ characteristics of MSTN and FGF5 dual-gene knockout sheep

Chen

Zhao

et al. 2023

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

IntroductionMyostatin (MSTN) negatively regulates skeletal muscle development. However, its function in reproductive performance and visceral organs has not been thoroughly investigated. Previously, we prepared a MSTN and fibroblast growth factor 5 (FGF5) double-knockout sheep, which was a MSTN and FGF5 dual-gene biallelic homozygous (MF−/−) mutant.MethodsTo understand the role of MSTN and FGF5 in reproductive performance and visceral organs, this study evaluated the ejaculation amount, semen pH, sperm motility, sperm density, acrosome integrity, rate of teratosperm, and seminal plasma biochemical indicators in adult MF−/− rams. We also compared the overall morphology, head, head-neck junction, middle segment and the transection of middle segment of spermatozoa between wildtype (WT) and MF−/− rams.ResultsOur results showed that the seminal plasma biochemical indicators, sperm structure and all sperm indicators were normal, and the fertilization rate also has no significant difference between WT and MF−/− rams, indicating that the MF−/− mutation did not affect the reproductive performance of sheep. Additional analysis evaluated the histomorphology of the visceral organs, digestive system and reproductive system of MF+/− sheep, the F1 generation of MF−/−, at the age of 12 months. There was an increased spleen index, but no significant differences in the organ indexes of heart, liver, lung, kidney and stomach, and no obvious differences in the histomorphology of visceral organs, digestive system and reproductive system in MF+/− compared with WT sheep. No MF+/− sheep were observed to have any pathological features.DiscussionIn summary, the MSTN and FGF5 double-knockout did not affect reproductive performance, visceral organs and digestive system in sheep except for differences previously observed in muscle and fat. The current data provide a reference for further elucidating the application of MSTN and FGF5 double-knockout sheep.

show abstract

The food safety assessment of all-female common carp (Cyprinus carpio) (cyp17a1+/−;XX genotype) generated using genome editing technology

Chen,

Huang,

Jia

et al. 2023

Food and Chemical Toxicology

View full text Add to dashboard Cite

A 90-Day Safety Study of Meat from MSTN and FGF5 Double-Knockout Sheep in Wistar Rats

Cited by 4 publications

References 29 publications

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Reproduction and viscera organ characteristics of MSTN and FGF5 dual-gene knockout sheep

The food safety assessment of all-female common carp (Cyprinus carpio) (cyp17a1+/−;XX genotype) generated using genome editing technology

Contact Info

Product

Resources

About