A 6-Year-Old Boy with COVID-19-Positive Pleural Effusion and Kawasaki-Like Features

Yousefzadegan, Sedigheh; Mahmoudabadi, Ramin Zare; Gharehbaghi, Golnaz

doi:10.1155/2021/8832826

Cited by 2 publications

(2 citation statements)

References 5 publications

(6 reference statements)

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“…SARS-CoV-2 has also been previously detected by RT-PCR in pleural uid, showing reactive mesothelial cells and lymphocytes in cytology examination, in patients with lung in ltrates 26 , and in children 27 . Viral RNA can also be detected in pleural uid in patients without lung parenchyma involvement 28 .…”

Section: Discussionmentioning

confidence: 83%

Identification of SARS-CoV-2 in different human tissues. Validation of immunohistochemical and qPCR techniques in paraffin-embedded tissues and cytology

Bernal-Florindo

Santisteban‐Espejo

Rio-Ignacio

et al. 2022

Preprint

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Since the beginning of 2020, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for a global pandemic. Although the scientific community has focused on its study, there is little knowledge on validated techniques for detecting SARS-CoV-2 in formalin-fixed paraffin embedded (FFPE) tissues archived in pathology departments. The objective of this study was to validate immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR) for the diagnosis of respiratory coronavirus disease 2019 (COVID-19) in cytology and FFPE tissues, obtained from multiple specimen types (autopsy, incisional biopsy and surgical specimens). We also defined relevant temporary points (interval, persistence and archival times) to evaluate the correlation between those periods and viral load. A total of 43 cytology and FFPE samples from patients with a previous positive qPCR COVID-19 test in nasopharyngeal swabs were analysed. Two different qPCR techniques were evaluated from IDT Technologies (method A) and Roche Diagnostics (method B). In immunohistochemistry, antibodies directed against nucleocapsid and spike viral proteins were employed. A total of 25.58% of the evaluated samples were positive for any of the two qPCR techniques. Only one placental specimen (3.44%) with acute villitis showed strong IHC positivity for both antibodies, and the other 4 specimens (lung, brain, kidney and placenta) showed isolated weakly positive cells with IHC. A strong statistically significant correlation was observed between threshold values for qPCR method A and interval time (p = 0.01; ρ = 0.917) and persistence time (p = 0.002; ρ = 0.879) and between qPCR method B and archival time (p = 0.037; ρ = 0.900). In conclusion, the qPCR technique is a sensitive diagnostic tool for SARS-COV-2 detection for both cytology and FFPE specimens. With currently available antibodies, IHC to detect the presence of SARS-COV-2 should only be used in special cases.

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Section: Discussionmentioning

confidence: 83%

Identification of SARS-CoV-2 in different human tissues. Validation of immunohistochemical and qPCR techniques in paraffin-embedded tissues and cytology

Bernal-Florindo

Santisteban‐Espejo

Rio-Ignacio

et al. 2022

Preprint

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“…The case series by Rostad et al [8] described an incidence of pleural effusions in MIS-C children of 81.8% (the majority of the pleural effusions (67%) were bilateral) compared to 6.3% in COVID-19 infections (P < 0.01). The cytokine storm in COVID-19 patients can occur rapidly, with immune cells attacking the lungs soon after MIS-C [9].…”

Section: Discussionmentioning

confidence: 99%

COVID-19-induced multisystem inflammatory syndrome in a child with Wilson disease: a case report

Abdel-Ghaffar

Zakaria

El-Sayed

et al. 2022

Egypt Liver Journal

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Background Infection with coronavirus disease 2019 (COVID-19) can progress to the multisystem inflammatory syndrome in children (MIS-C). Patients with liver cirrhosis are at increased risk of complications. Case presentation We report on a 13-year-old Wilson’s disease patient who was referred for liver transplantation because of rapid deterioration in his hepatic condition. After admission, he developed fever, respiratory distress, coronary arteries dilatation on echocardiography, laboratory evidence of inflammation, and positive severe acute respiratory syndrome coronavirus (SARS-CoV-2) PCR. SARS-CoV-2-induced MIS-C was diagnosed. Inspite of aggressive management of MIS-C, progressive deterioration of the respiratory, liver, kidney, and cardiac functions occurred and he passed away. Conclusion MIS-C is a serious possible complication leading to multiorgan failure and higher death rate especially in cirrhotic children. So, early diagnosis and management with higher level of care by a multidisciplinary team are warranted.

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A 6-Year-Old Boy with COVID-19-Positive Pleural Effusion and Kawasaki-Like Features

Cited by 2 publications

References 5 publications

Identification of SARS-CoV-2 in different human tissues. Validation of immunohistochemical and qPCR techniques in paraffin-embedded tissues and cytology

Identification of SARS-CoV-2 in different human tissues. Validation of immunohistochemical and qPCR techniques in paraffin-embedded tissues and cytology

COVID-19-induced multisystem inflammatory syndrome in a child with Wilson disease: a case report

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