A 3D-printed PRP-GelMA hydrogel promotes osteochondral regeneration through M2 macrophage polarization in a rabbit model

Jiang, Guangyao; Li, Sihao; Kang, Yu Min; He, Bin; Hong, Jianqiao; Xu, Tengjing; Meng, Jinghui; Ye, Chenyi; Chen, Yazhou; Shi, Zhijun; Feng, Gang; Chen, Weishan; Yan, Shigui; He, Yong; Yan, Ruijian

doi:10.1016/j.actbio.2021.04.010

Cited by 153 publications

(127 citation statements)

References 56 publications

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“…Initially, macrophages show M1 phenotypes, which possess the ability to phagocytose dead cells and pathogens and promote inflammation, and then they transform to anti‐inflammatory M2 phenotypes, which could stimulate osteogenesis. [ 213 ] Present studies mainly focused on inhibiting M1 macrophages and promoting M2 macrophages. For example, IL‐4 is a well‐characterized cytokine that could activate M2 phenotypes, and it was first loaded on graphene oxide (GO) coupled with BMP‐2 for controlled release.…”

Section: Biochemical Functionalization For Multifunctional Microenvir...mentioning

confidence: 99%

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

Hao

Wang

et al. 2022

Advanced Science

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Bone tissue engineering is becoming an ideal strategy to replace autologous bone grafts for surgical bone repair, but the multihierarchical complexity of natural bone is still difficult to emulate due to the lack of suitable biomaterials. Supramolecular peptide nanofiber hydrogels (SPNHs) are emerging biomaterials because of their inherent biocompatibility, satisfied biodegradability, high purity, facile functionalization, and tunable mechanical properties. This review initially focuses on the multihierarchical fabrications by SPNHs to emulate natural bony extracellular matrix. Structurally, supramolecular peptides based on distinctive building blocks can assemble into nanofiber hydrogels, which can be used as nanomorphology-mimetic scaffolds for tissue engineering. Biochemically, bioactive motifs and bioactive factors can be covalently tethered or physically absorbed to SPNHs to endow various functions depending on physiological and pharmacological requirements. Mechanically, four strategies are summarized to optimize the biophysical microenvironment of SPNHs for bone regeneration. Furthermore, comprehensive applications about SPNHs for bone tissue engineering are reviewed. The biomaterials can be directly used in the form of injectable hydrogels or composite nanoscaffolds, or they can be used to construct engineered bone grafts by bioprinting or bioreactors. Finally, continuing challenges and outlook are discussed.

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Section: Biochemical Functionalization For Multifunctional Microenvir...mentioning

confidence: 99%

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

Hao

Wang

et al. 2022

Advanced Science

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“…In addition, PRP induces autocrine growth factors to promote cartilage healing ( Chang et al, 2018 ). Jiang et al (2021) suggested that PRP can promote osteochondral regeneration by promoting the polarization of M2 macrophages. Accordingly, they prepared PRP-GelMA hydrogel scaffolds inoculated with rabbit BMSCs and observed an increase in M2 macrophage, which had an anti-inflammatory effect and provided a favorable environment for osteochondral regeneration ( Jiang et al, 2021 ).…”

Section: 3d Printingmentioning

confidence: 99%

3D Printing for Bone-Cartilage Interface Regeneration

Jiao

et al. 2022

Front. Bioeng. Biotechnol.

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Due to the vasculature defects and/or the avascular nature of cartilage, as well as the complex gradients for bone-cartilage interface regeneration and the layered zonal architecture, self-repair of cartilage and subchondral bone is challenging. Currently, the primary osteochondral defect treatment strategies, including artificial joint replacement and autologous and allogeneic bone graft, are limited by their ability to simply repair, rather than induce regeneration of tissues. Meanwhile, over the past two decades, three-dimension (3D) printing technology has achieved admirable advancements in bone and cartilage reconstruction, providing a new strategy for restoring joint function. The advantages of 3D printing hybrid materials include rapid and accurate molding, as well as personalized therapy. However, certain challenges also exist. For instance, 3D printing technology for osteochondral reconstruction must simulate the histological structure of cartilage and subchondral bone, thus, it is necessary to determine the optimal bioink concentrations to maintain mechanical strength and cell viability, while also identifying biomaterials with dual bioactivities capable of simultaneously regenerating cartilage. The study showed that the regeneration of bone-cartilage interface is crucial for the repair of osteochondral defect. In this review, we focus on the significant progress and application of 3D printing technology for bone-cartilage interface regeneration, while also expounding the potential prospects for 3D printing technology and highlighting some of the most significant challenges currently facing this field.

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“…Hydrogel can also promote bone healing with a reduced inflammatory response. In addition to BMP-2, other growth factors from platelet-rich plasma (PRP), such as IGF, PDGF, and TGF- β , may improve M2 phenotype polarization, which then enhances bone regeneration [ 178 ].…”

Section: Modulations Of the Msc–immune Cell Interaction For Bone Tiss...mentioning

confidence: 99%

Mesenchymal Stem Cell–Immune Cell Interaction and Related Modulations for Bone Tissue Engineering

Chen

Hao

Wang

et al. 2022

Stem Cells International

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Critical bone defects and related delayed union and nonunion are still worldwide problems to be solved. Bone tissue engineering is mainly aimed at achieving satisfactory bone reconstruction. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells that can differentiate into bone cells and can be used as one of the key pillars of bone tissue engineering. In recent decades, immune responses play an important role in bone regeneration. Innate immune responses provide a suitable inflammatory microenvironment for bone regeneration and initiate bone regeneration in the early stage of fracture repair. Adaptive immune responses maintain bone regeneration and bone remodeling. MSCs and immune cells regulate each other. All kinds of immune cells and secreted cytokines can regulate the migration, proliferation, and osteogenic differentiation of MSCs, which have a strong immunomodulatory ability to these immune cells. This review mainly introduces the interaction between MSCs and immune cells on bone regeneration and its potential mechanism, and discusses the practical application in bone tissue engineering by modulating this kind of cell-to-cell crosstalk. Thus, an in-depth understanding of these principles of bone immunology can provide a new way for bone tissue engineering.

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A 3D-printed PRP-GelMA hydrogel promotes osteochondral regeneration through M2 macrophage polarization in a rabbit model

Cited by 153 publications

References 56 publications

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

3D Printing for Bone-Cartilage Interface Regeneration

Mesenchymal Stem Cell–Immune Cell Interaction and Related Modulations for Bone Tissue Engineering

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