A 3D polydimethylsiloxane microhourglass-shaped channel array made by reflowing photoresist structures for engineering a blood capillary network

Rho, Hoon Suk; Veltkamp, Henk-Willem; Baptista, Danielle; Gardeniers, Han; Gac, Séverine Le; Habibović, Pamela

doi:10.1016/j.ymeth.2020.03.007

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…A glass-bonded polydimethylsiloxane (PDMS) microfluidic flow-focusing droplet generator was fabricated using standard soft lithography ( Xia and Whitesides, 1998 ; Rho et al, 2021 ). In brief, the photomask design was prepared using CleWin software (WieWeb software) and printed on a 5-inch soda lime glass using an LBPG Heidelberg DWL200 mask generator (Heidelberg Instruments Mikrotechnik GmbH).…”

Section: Methodsmentioning

confidence: 99%

Optimization of a tunable process for rapid production of calcium phosphate microparticles using a droplet-based microfluidic platform

Alaoui Selsouli,

Rho,

Eischen-Loges

et al. 2024

Front. Bioeng. Biotechnol.

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Calcium phosphate (CaP) biomaterials are amongst the most widely used synthetic bone graft substitutes, owing to their chemical similarities to the mineral part of bone matrix and off-the-shelf availability. However, their ability to regenerate bone in critical-sized bone defects has remained inferior to the gold standard autologous bone. Hence, there is a need for methods that can be employed to efficiently produce CaPs with different properties, enabling the screening and consequent fine-tuning of the properties of CaPs towards effective bone regeneration. To this end, we propose the use of droplet microfluidics for rapid production of a variety of CaP microparticles. Particularly, this study aims to optimize the steps of a droplet microfluidic-based production process, including droplet generation, in-droplet CaP synthesis, purification and sintering, in order to obtain a library of CaP microparticles with fine-tuned properties. The results showed that size-controlled, monodisperse water-in-oil microdroplets containing calcium- and phosphate-rich solutions can be produced using a flow-focusing droplet-generator microfluidic chip. We optimized synthesis protocols based on in-droplet mineralization to obtain a range of CaP microparticles without and with inorganic additives. This was achieved by adjusting synthesis parameters, such as precursor concentration, pH value, and aging time, and applying heat treatment. In addition, our results indicated that the synthesis and fabrication parameters of CaPs in this method can alter the microstructure and the degradation behavior of CaPs. Overall, the results highlight the potential of the droplet microfluidic platform for engineering CaP microparticle biomaterials with fine-tuned properties.

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Section: Methodsmentioning

confidence: 99%

Optimization of a tunable process for rapid production of calcium phosphate microparticles using a droplet-based microfluidic platform

Alaoui Selsouli,

Rho,

Eischen-Loges

et al. 2024

Front. Bioeng. Biotechnol.

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“…However, this approach does not capture the intrinsic curvature of the vasculature. By contrast, cylindrical structures in hydrogels or channels [115] incorporate this curvature. For instance, endothelial cells (ECs) have been seeded in lumens previously formed in a hydrogel matrix using (i) needles, metal rods, or fishing lines [116] that are removed after gelation, (ii) sacrificial materials such as gelatin or 3D-printed carbohydrates [117], or (iii) the viscous finger patterning technique (Figure IB).…”

Section: Trends Trends In In Biotechnology Biotechnologymentioning

confidence: 99%

Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication

Picollet-D’hahan

Żuchowska

Lemeunier

et al. 2021

Trends in Biotechnology

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209

151

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Multiorgan-on-a-chip (multi-OoC) platforms have great potential to redefine the way in which human health research is conducted. After briefly reviewing the need for comprehensive multiorgan models with a systemic dimension, we highlight scenarios in which multiorgan models are advantageous. We next overview existing multi-OoC platforms, including integrated body-on-a-chip devices and modular approaches involving interconnected organ-specific modules. We highlight how multi-OoC models can provide unique information that is not accessible using single-OoC models. Finally, we discuss remaining challenges for the realization of multi-OoC platforms and their worldwide adoption. We anticipate that multi-OoC technology will metamorphose research in biology and medicine by providing holistic and personalized models for understanding and treating multisystem diseases.

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“…A minimum channel diameter of 200 μm was reported . More frequently, the well-known temperature reflow process of ridges made on thermoplastic materials has been used. ,, However, this method offers poor control of the channel cross section. An innovative approach was described by Vecchione et al ., whereby round-shaped channels were produced by spin-coating PDMS on microfabricated square trenches; as the PDMS due to surface tension accumulated at the corners, the square channels became rounded .…”

Section: Introductionmentioning

confidence: 99%

Microvessel-on-Chip Fabrication for the In Vitro Modeling of Nanomedicine Transport

2021

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Tumor-on-chip devices are becoming ideal platforms to recreate in vitro the particular physiological microenvironment of interest for onco-nanomedicine testing and development. This work presents a strategy to produce a round artificial microvessel on-a-chip device for the study of physiologically relevant nanomedicine transport dynamics. The microchannels have a diameter in the range of the tumor capillaries and a semicircular geometry. This geometry is obtained through an intermediate thermal nanoimprint step using a master mold with square-shaped channel structures produced by standard silicon micromachining or by stereolithography three-dimensional (3D) printing. The working microfluidic chip devices are made by casting polydimethylsiloxane on the imprinted intermediate mold. Artificial blood microvessels are created by seeding human endothelial cells into the round-shaped channels acting as the scaffold. The microchip is connected by 3D-printed reservoirs to a pressure controller, allowing for a fine fluidic control. Under physiological flow conditions, the dynamic interaction of nanoparticles (NPs) with the artificial endothelium was assessed by high-magnification fluorescence microscopy. Overtime, internalization of NPs and clustering was observed and the accumulation rate into the endothelial cells could be characterized in real time.

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A 3D polydimethylsiloxane microhourglass-shaped channel array made by reflowing photoresist structures for engineering a blood capillary network

Cited by 4 publications

References 41 publications

Optimization of a tunable process for rapid production of calcium phosphate microparticles using a droplet-based microfluidic platform

Optimization of a tunable process for rapid production of calcium phosphate microparticles using a droplet-based microfluidic platform

Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication

Microvessel-on-Chip Fabrication for the In Vitro Modeling of Nanomedicine Transport

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