A 3D feature point tracking method for ion radiation

Kouwenberg, J.J.M.; Ulrich, Leonie; Jäkel, Oliver; Greilich, Steffen

doi:10.1088/0031-9155/61/11/4088

Cited by 19 publications

(23 citation statements)

References 29 publications

(40 reference statements)

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“…The zoom was set to 1.1 to decrease vignetting effects at the imaging margin. For image processing purposes the single tiles comprising an imaging stack of 20 layers were normalized using the in-house written software called FNTD package [18]. All tiles were recorded with a spatial overlap of approximately 20 × 193.5 µm 2 .…”

Section: Methodsmentioning

confidence: 99%

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The biomedical sensor Cell-Fit-HD^4D, reveals individual tumor cell fate in response to microscopic ion deposition

Niklas

Schlegel

Liew

et al. 2020

Preprint

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Here we present the biomedical sensor cell-fluorescent ion track hybrid detector4D (Cell-Fit-HD4D) to reveal individual tumor cell fate in response to microscopic ion deposition in ion beam therapy. The sensor enables long-term monitoring of single tumor cells after clinical ion beam irradiation in combination with single-cell dosimetry. Cell-Fit-HD4D is read out in-situ by conventional optical microscopy. Direct visualization of a clinical ion beam is hereby possible for the first time. The possibility to reveal fate of individual cells from a cell cohort demonstrates that our biomedical sensor clearly differs from conventional experiments that characterize cellular response after radiation on a population level. Cell-Fit-HD4D is therefore used to mimics the clinical situation of a defined tumor depth during tumor treatment by ion beam therapy. Our biomedical sensor is able to provide crucial input for current mechanistic approaches to biophysical modelling of the effect of ionizing radiation on biological matter. In the clinical context, obtaining multi-dimensional physical and biological information on individual tumor cells is an important step to further transform ion beam therapy into a highly precise discipline within oncology.

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Section: Methodsmentioning

confidence: 99%

“…The images obtained from the FNTD were analysed by in-house written software called FNTD package [18]. This software can be run as a plugin for the image-processing and analysis software ImageJ/Fiji .…”

Section: Methodsmentioning

confidence: 99%

The biomedical sensor Cell-Fit-HD^4D, reveals individual tumor cell fate in response to microscopic ion deposition

Niklas

Schlegel

Liew

et al. 2020

Preprint

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“…The IX83 was used to image a 0.5 Gy carbon ion irradiated FNTD. The resulting WF images were analysed using the FNTD analysis software developed and implemented in ImageJ [13,17]. To ensure that no bias or artifacts were introduced into the analysis, the raw data was used.…”

Section: Microscopy and Image Analysismentioning

confidence: 99%

Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy

Walsh

Liew

Schlegel

et al. 2020

Preprint

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Fluorescent nuclear track detectors (FNTD) are solid-state dosimeters used in a wide range of dosimetric and biomedical applications in research worldwide. FNTD's are a core but currently underutilized dosimetry tool in the field of radiation biology which are inherently capable of visualizing the tracks of ions used in hadron therapy. The ions that traverse the FNTD deposit their energy according to their linear energy transfer (LET) and transform colour centres to form trackspots around their trajectory. These trackspots are the fingerprints of the ions which have fluorescent properties (620 nm peak excitation and 750 nm emission). These properties enable an analysis of the trackspots in the FNTD using fluorescence microscopy enables a well-defined dosimetric readout with a spatial component indicating the trajectory of individual ions. The current method used to analyse the FNTDs is laser scanning confocal microscopy (LSM). This imaging technique enables a precise localization and imaging of track spots in x, y and z however due to the scanning of a single laser spot across the sample requires a lot of time for large samples. This body of work conclusively shows for the first time that the readout of the trackspots present after irradiation (0.5 Gy carbon ions) in the FNTD can be captured with a widefield microscope (WF). This method was developed to optimize the readout of the Cell-FIT-HD biomedical sensor based on a Landauer Al2O3:C,Mg FNTD which is used for biological single cell dosimetry and tracking using widefield microscopy. The cells to be analysed are seeded onto an FNTD and irradiated with carbon ions, then the FNTD and the cells can be imaged using an LSM and the WF microscope respectively. The capture of the two separate readouts, cells and tracks, on two separate microscopes is not only time intensive but also introduces a spatial error between tracks and cells which needs to be corrected. The work in this paper now enables the use of a single widefield microscope to image the cells growing on the FNTD and the tracks in the FNTD. In comparison to the current LSM system the WF readout of the FNTD is a factor ~10 faster, for an area 2.97 times the size making the method nearly a factor 19 faster in track acquisition. This WF setup will replace LSM in the Cell-FIT-HD for FNTD track readout, which will enable a higher throughput of samples as well as a reduced error in the correlation between tracks and cells and enable a high throughput readout of Cell-FIT-HD.

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“…This would provide a comprehensive characterization of the carbon ion beam . Additionally, fluorescent nuclear track detectors (FNTD) have been applied to measure the integral energy deposited by individual particle tracks, which can be used to investigate LET . Those detectors can even be coated with cell cultures .…”

Section: Advanced Measurementsmentioning

confidence: 99%

“…33 Additionally, fluorescent nuclear track detectors (FNTD) have been applied to measure the integral energy deposited by individual particle tracks, which can be used to investigate LET. 34,35 Those detectors can even be coated with cell cultures. 36 However, one challenge with such assessments of LET is how to make LET assessments traceable to a national standard.…”

Section: A Overviewmentioning

confidence: 99%

Report of a National Cancer Institute special panel: Characterization of the physical parameters of particle beams for biological research

et al. 2018

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Purpose To define the physical parameters needed to characterize a particle beam in order to allow intercomparison of different experiments performed using different ions at the same facility and using the same ion at different facilities. Methods At the request of the National Cancer Institute (NCI), a special panel was convened to review the current status of the field and to provide suggested metrics for reporting the physical parameters of particle beams to be used for biological research. A set of physical parameters and measurements that should be performed by facilities and understood and reported by researchers supported by NCI to perform pre‐clinical radiobiology and medical physics of heavy ions were generated. Results Standard measures such as radiation delivery technique, beam modifiers used, nominal energy, field size, physical dose and dose rate should all be reported. However, more advanced physical measurements, including detailed characterization of beam quality by microdosimetric spectrum and fragmentation spectra, should also be established and reported. Details regarding how such data should be incorporated into Monte Carlo simulations and the proper reporting of simulation details are also discussed. Conclusions In order to allow for a clear relation of physical parameters to biological effects, facilities and researchers should establish and report detailed physical characteristics of the irradiation beams utilized including both standard and advanced measures. Biological researchers are encouraged to actively engage facility staff and physicists in the design and conduct of experiments. Modeling individual experimental setups will allow for the reporting of the uncertainties in the measurement or calculation of physical parameters which should be routinely reported.

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A 3D feature point tracking method for ion radiation

Cited by 19 publications

References 29 publications

The biomedical sensor Cell-Fit-HD^4D, reveals individual tumor cell fate in response to microscopic ion deposition

The biomedical sensor Cell-Fit-HD^4D, reveals individual tumor cell fate in response to microscopic ion deposition

Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy

Report of a National Cancer Institute special panel: Characterization of the physical parameters of particle beams for biological research

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A 3D feature point tracking method for ion radiation

Cited by 19 publications

References 29 publications

The biomedical sensor Cell-Fit-HD4D, reveals individual tumor cell fate in response to microscopic ion deposition

The biomedical sensor Cell-Fit-HD4D, reveals individual tumor cell fate in response to microscopic ion deposition

Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy

Report of a National Cancer Institute special panel: Characterization of the physical parameters of particle beams for biological research

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Product

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The biomedical sensor Cell-Fit-HD^4D, reveals individual tumor cell fate in response to microscopic ion deposition

The biomedical sensor Cell-Fit-HD^4D, reveals individual tumor cell fate in response to microscopic ion deposition