A 3D bioprinted decellularized extracellular matrix/gelatin/quaternized chitosan scaffold assembling with poly(ionic liquid)s for skin tissue engineering

Xu, Jie; Fang, Huan; Su, Ya; Kang, Yue; Xu, De-Li; Cheng, Yuen Yee; Nie, Yi; Wang, Hong; Liu, Tianqing; Song, Kedong

doi:10.1016/j.ijbiomac.2022.08.149

Cited by 29 publications

(13 citation statements)

References 60 publications

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“…As naturally-derived biomaterials, hyaluronic acid [ 2 , 3 ] and gelatin [ 4 ] are examples of hydrophilic polymers that have found widespread usage in biomedical applications due to their biocompatibility, biodegradability, and non-cytotoxicity. Moreover, hydrogels based on gelatin, a biocompatible product of collagen obtained through hydrolysis [ 5 ], which has a significant role in the proliferation of fibroblasts at wound sites, have been regarded as one of the most promising candidates for wound care.…”

Section: Introductionmentioning

confidence: 99%

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Rusu

Niţă

Simiónescu³

et al. 2023

Polymers

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Development of natural protein-based hydrogels with self-healing performance and tunable physical properties has attracted increased attention owing to their wide potential not only in the pharmaceutical field, but also in wounds management. This work reports the development of a versatile hydrogel based on enzymatically-crosslinked gelatin and nanogels loaded with amoxicillin (Amox), an antibiotic used in wound infections. The transglutaminase (TGase)-crosslinked hydrogels and encapsulating nanogels were formed rapidly through enzymatic crosslinking and self-assembly interactions in mild conditions. The nanogels formed through the self-assemble of maleoyl-chitosan (MAC5) and polyaspartic acid (PAS) may have positive influence on the self-healing capacity and drug distribution within the hydrogel network through the interactions established between gelatin and gel-like nanocarriers. The physicochemical properties of the enzymatically-crosslinked hydrogels, such as internal structure, swelling and degradation behavior, were studied. In addition, the Amox release studies indicated a rapid release when the pH of the medium decreased, which represents a favorable characteristic for use in the healing of infected wounds. It was further observed through the in vitro and in vivo biocompatibility assays that the optimized scaffolds have great potential to be used as wound dressings.

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Section: Introductionmentioning

confidence: 99%

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Rusu

Niţă

Simiónescu³

et al. 2023

Polymers

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“…The rheological properties of bioinks were tested by a rheometer (Anton-Paar, Austria) [ 37 ] . To determine the viscoelasticity of the bioinks, the measuring position was set to 0.1 mm, and the angular frequency range was 0.01–100 rad∙s −1 .…”

Section: Methodsmentioning

confidence: 99%

A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink

Xu¹,

Yang²,

Su³

et al. 2022

IJB

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Tissue-engineered scaffolds are more commonly used to construct three-dimensional (3D) tumor models for in vitro studies when compared to the conventional two-dimensional (2D) cell culture because the microenvironments provided by the 3D tumor models closely resemble the in vivo system and could achieve higher success rate when the scaffolds are translated for use in pre-clinical animal model. Physical properties, heterogeneity, and cell behaviors of the model could be regulated to simulate different tumors by changing the components and concentrations of materials. In this study, a novel 3D breast tumor model was fabricated by bioprinting using a bioink that consists of porcine liver-derived decellularized extracellular matrix (dECM) with different concentrations of gelatin and sodium alginate. Primary cells were removed while extracellular matrix components of porcine liver were preserved. The rheological properties of biomimetic bioinks and the physical properties of hybrid scaffolds were investigated, and we found that the addition of gelatin increased hydrophilia and viscoelasticity, while the addition of alginate increased mechanical properties and porosity. The swelling ratio, compression modulus, and porosity could reach 835.43 ± 130.61%, 9.64 ± 0.41 kPa, and 76.62 ± 4.43%, respectively. L929 cells and the mouse breast tumor cells 4T1 were subsequently inoculated to evaluate biocompatibility of the scaffolds and to form the 3D models. The results showed that all scaffolds exhibited good biocompatibility, and the average diameter of tumor spheres could reach 148.52 ± 8.02 μm on 7 d. These findings suggest that the 3D breast tumor model could serve as an effective platform for anticancer drug screening and cancer research in vitro.

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“…The scaffold showed 100% of antibacterial activity against E. coli (Gram-negative) and S. aureus (Gram- negative) bacteria, and good hemostatic and hemocompatibility properties when compared with a quaternized CHT-free control scaffold. Additionally, it allowed the growth of fibroblasts, showing a higher ECM deposition, in particular, in terms of collagen type I, fibronectin and decorin [ 300 ].…”

Section: Chitosan-based Inks For 3d Printing Applicationsmentioning

confidence: 99%

“…NS: p > 0.05, * p < 0.05, ** p < 0.01); ( e ) images of the antibacterial properties of the scaffolds against E. coli and S. aureus . Reprinted and adapted with permission from [ 300 ] © 2022 Elsevier B.V.…”

Section: Figures and Schemesmentioning

confidence: 99%

Chitosan-Based Biomaterials: Insights into Chemistry, Properties, Devices, and Their Biomedical Applications

et al. 2023

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Chitosan is a marine-origin polysaccharide obtained from the deacetylation of chitin, the main component of crustaceans’ exoskeleton, and the second most abundant in nature. Although this biopolymer has received limited attention for several decades right after its discovery, since the new millennium chitosan has emerged owing to its physicochemical, structural and biological properties, multifunctionalities and applications in several sectors. This review aims at providing an overview of chitosan properties, chemical functionalization, and the innovative biomaterials obtained thereof. Firstly, the chemical functionalization of chitosan backbone in the amino and hydroxyl groups will be addressed. Then, the review will focus on the bottom-up strategies to process a wide array of chitosan-based biomaterials. In particular, the preparation of chitosan-based hydrogels, organic–inorganic hybrids, layer-by-layer assemblies, (bio)inks and their use in the biomedical field will be covered aiming to elucidate and inspire the community to keep on exploring the unique features and properties imparted by chitosan to develop advanced biomedical devices. Given the wide body of literature that has appeared in past years, this review is far from being exhaustive. Selected works in the last 10 years will be considered.

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A 3D bioprinted decellularized extracellular matrix/gelatin/quaternized chitosan scaffold assembling with poly(ionic liquid)s for skin tissue engineering

Cited by 29 publications

References 60 publications

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink

Chitosan-Based Biomaterials: Insights into Chemistry, Properties, Devices, and Their Biomedical Applications

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