1992
DOI: 10.1111/j.1365-2818.1992.tb03217.x
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A 360° single‐axis tilt stage for the high‐voltage electron microscope

Abstract: A new type of specimen stage that permits more than 180' of tilting about the axis of a side-entry rod has been developed for a high-voltage electron microscope (HVEM). Roughly cylindrical specimens, with radial dimensions of less than a few micrometres, that can be mounted on the tip of a microneedle or micropipette are applicable. For glass micropipettes, the energy of the 1-MeV beam of the HVEM is sufficient to image specimens through both walls. T h e stage employs a spindle mechanism that holds these need… Show more

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Cited by 51 publications
(17 citation statements)
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References 24 publications
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“…This goal will require improving current alignment schemes (in progress) as well as seeking ways to fill in the missing angular range of tilts. An approach to the latter problem is the development of cylindrical specimen holders for collection of images over the full 180" tilt range (Barnard et al, 1992). Finally, because of concerns regarding the effects of specimen preparative techniques on mitochondrial structure, low-denaturation and cryosubstitution protocols will be employed for optimizing structural preservation.…”
Section: Resultsmentioning
confidence: 99%
“…This goal will require improving current alignment schemes (in progress) as well as seeking ways to fill in the missing angular range of tilts. An approach to the latter problem is the development of cylindrical specimen holders for collection of images over the full 180" tilt range (Barnard et al, 1992). Finally, because of concerns regarding the effects of specimen preparative techniques on mitochondrial structure, low-denaturation and cryosubstitution protocols will be employed for optimizing structural preservation.…”
Section: Resultsmentioning
confidence: 99%
“…This might involve the fabrication of cooled micromanipulators for in situ 'lift out' procedures ( Fig. 5, similar to those known from TEM lamella preparations at room temperature) and the fabrication of cylindrical milling geometries that can be used in conjunction with rotation-tilt tomography holders, which in turn, do not suffer from an increase in specimen thickness during rotation, as experienced with a 'slab' geometry (Barnard et al, 1992;Kamino et al, 2004).…”
Section: Discussionmentioning
confidence: 98%
“…Thus, it is possible to generate planar or crosssectional thin sections, or even cylindrically symmetric specimens for electron tomography. Cylindrically symmetric specimens, mounted such that the long axis is oriented along the microscope tilt axis, can be superior to flat sections for purposes of structure determination using electron tomography, because the loss in vertical resolution due to the missing wedge effect can be largely eliminated (Barnard et al, 1992;Lee and Subramaniam, 2004). The capability of site-specific milling provides a potentially interesting alternative to the use of manually operated microtomes for the generation of sections for histological examination using either electron or light microscopy.…”
Section: Discussionmentioning
confidence: 99%