A 3-year field investigation of impacts of Monsanto’s transgenic Bt-cotton NC 33B on rhizosphere microbial communities in northern China

Zhang, Yanjun; Xie, Ming; Wu, Gang; Peng, Deliang; Wang, Yu

doi:10.1016/j.apsoil.2015.01.003

Cited by 25 publications

(16 citation statements)

References 31 publications

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“…Most studies proved that transgenic Bt crops had no significant effects on microbial population sizes in soil, or only had the transient effects (Donegan et al 1995, Saxena and Stotzky 2002. Our results were in accordance not only with the above studies that the transgenic trait had no significant or minor effects on microbial population size in rhizosphere soil, but also a recent report that the transgenic cotton NC 33B has no apparent impact on the population size of microorganisms in soil (expressed as the CFUs by the selective cultivation method) (Zhang et al 2015). By contrast, another study reported that the Bt-trait had significant effects on the population size of actinomycetes (16S rDNA gene copies detected by qPCR) in rhizosphere soil of eggplant (Singh et al 2013).…”

Section: Resultssupporting

confidence: 92%

“…Kurstaki, was the first introduced transgenic Bt cotton in China, and had been adopted in northern China since its commercial release in 1997 (Wu et al 2003). No significant changes in the eubacterial, fungal and actinomycetes population size (expressed as the CFUs by the selective cultivation method) was found in rhizosphere soil of NC 33B (Zhang et al 2015). However, the results based on the cultivation presented should be considered only preliminary because a culture-based technique detects only a small portion of the microbial community.…”

mentioning

confidence: 99%

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Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cotton

Zhang¹,

Xie²,

Peng³

et al. 2016

PLANT SOIL ENVIRON

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Section: Resultssupporting

confidence: 92%

mentioning

confidence: 99%

Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cotton

Zhang¹,

Xie²,

Peng³

et al. 2016

PLANT SOIL ENVIRON

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“…It was also reported that P. kudriavzevii is responsible for producing offflavour odours and texture softening in kimchi (Moon et al, 2014), is one of the yeast producing terpenoids in Chinese light-style liquor (Wu et al, 2015). Both P. kudriavzevii and G. geotrichum are present in Chinese liquor fermentation starter Daqu and they participate in liquor flavour formation (Zhang et al, 2014).…”

Section: Pcr-dgge Fingerprinting and Sequencingmentioning

confidence: 96%

“…Recent papers applying this method include analysing the relationships between bacterial species in traditional fermentation starters for Hong Qu glutinous rice wine (Lv et al, 2012), microorganisms in Shanxi aged vinegar (Nie et al, 2015), microorganisms in the rhizosphere soil of Bt and non-Bt cottons in China (Zhang et al, 2015), microbial community in different Pu'er tea samples (Tian et al, 2013), bacteria present in different stages of the spontaneous ngari fermentation (dry fermented fish product) (Devi et al, 2015), correlation between volatile compounds and microorganisms during fermentation of yakupa (Brazilian fermented cassava beverage) (Freire et al, 2014), phyllosphere of lettuce (Zwielehner et al, 2008) and to discriminate farming types of nectarines and peaches (Bigot et al, 2015).…”

Section: Pcr-dgge Fingerprinting and Sequencingmentioning

confidence: 99%

Application of the PCR-DGGE technique to the fungal community of traditional Wielkopolska fried ripened curd cheese to determine its PGI authenticity

et al. 2017

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Fried ripened curd cheese is a traditional dairy product from the Wielkopolska region of Poland. It is made from cows' milk and has Protected Geographical Indication (PGI) status. The aim of this study was to use the microbial-culture-independent PCR-DGGE technique to examine whether a link exists between the fungal community present in the product and the geographical origin of the cheese. To look for correlations between the geographical origin and the microbiota composition, the DGGE fingerprinting profiles were subjected to a multivariate analysis, which included k-means clustering and principal component analysis (PCA). The dominant eukaryotic community of this traditional product, which has been investigated here for the first time, was shown to be composed of Galactomyces geotrichum, Kluyveromyces marxianus, Pichia kudriavzevii, Candida inconspicua, and Trichosporon spp. Our results demonstrated that PCR-DGGE is a simple and relatively inexpensive method for the traceability of Wielkopolska fried cheese and could be extended to other microbe-enriched foodstuffs. (Résumé d'auteur

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“…Inevitably, Bt toxin is introduced to soil primarily through the degradation of plant biomass remnants (Zhang et al, 2014), root exudates, through pollen deposition during tasseling, e.g., maize and by incorporation of plant residues after harvest (Zhang et al, 2015). There is no consensus about the persistence of the Cry proteins in soils.…”

Section: Introductionmentioning

confidence: 99%

Impact of transgenic cotton expressing cry1Ac and cry2Ab genes on soil rhizosphere bacterial and fungal populations in soils of central Kenya

Swilla¹,

Waturu²,

Rubindamayugi³

2016

Afr. J. Biotechnol.

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The impact of 9 months cultivation of transgenic Bacillus thuringiensis (Bt) cotton on selected culturable bacterial and fungal populations in rhizosphere soil was investigated. The transgenic Bt cotton line (06Z604M), isoline (99M03) and a non-Bt cotton line (HART 89M) were planted in confined field site in Samuru-Thika where non-Bt cotton had been continuously cultivated for the past 2 years. Rhizosphere soil samples were collected at planting, maturity, flower and boll, and boll-opening stages and harvesting stages of cotton. Numbers of culturable soil microbial groups (bacteria, actinomycetes and fungi) involved in decomposition and nutrients recycling were measured at CFU and population levels. The proteins did not show effects on bacterial, actinomycetes and fungal counts and populations possibly as a result of adsorption of the proteins on soil particles, which could have rendered the proteins inaccessible for microbial utilization. Culturable microbial population and colony counts arranged in decreasing order were 06Z604D>99M03>HART89M, similar to the amounts of MBC and clay in the soils. Moreover, bacteria and fungi counts were higher at 110 DAS in 06Z604D than in 99M03 and HART89M plot soils. Our observations suggest that insecticidal proteins (Cry1Ac and Cry2Ab2) produced by Bollgard II Bt cotton could persist in tropical soils as a result of adsorption on soil clays but that there were no observable effect on the studied culturable microbial groups.The data presented here showed no consistent statistically significant differences (p<0.005) in the numbers of different groups of culturable bacteria, actinomycetes and fungi between rhizosphere soil of Bt, isoline and Hart 89M cotton lines in the same field, and no obvious trends in the numbers of the culturable bacteria and fungi with the increasing growth duration. Moreover, the studied culturable bacterial and fungal groups were positively correlated (p>0.001) with soil respiration and microbial biomass, which exhibited uneven trend with the treatments. Generally Soil from 06Z604D showed the slight higher microbial populations and CFU count, whilst HART 89M showed slight lower microbial count. This depicts the fact that slight variability in the treatments, quality and content of the root exudates might have a temporal or permanent shift in micro biota populations of a variety of crop studied.This study therefore suggests that a single-year cultivation of transgenic Bt cotton may not affect the functional bacterial and fungi populations in rhizosphere soil.

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A 3-year field investigation of impacts of Monsanto’s transgenic Bt-cotton NC 33B on rhizosphere microbial communities in northern China

Cited by 25 publications

References 31 publications

Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cotton

Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cotton

Application of the PCR-DGGE technique to the fungal community of traditional Wielkopolska fried ripened curd cheese to determine its PGI authenticity

Impact of transgenic cotton expressing cry1Ac and cry2Ab genes on soil rhizosphere bacterial and fungal populations in soils of central Kenya

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