A 3-D microelectrode system for handling and caging single cells and particles

Müller, Torsten; Gradl, Gabriele; Howitz, S.; Shirley, Stephen G.; Schnelle, Thomas; Fuhr, G.

doi:10.1016/s0956-5663(99)00006-8

Cited by 233 publications

(181 citation statements)

References 24 publications

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“…It has been shown that particles can be focused in three dimensions using electric field cages, and also in twodimensions within a fluid stream [11][12][13]. A small channel, typically 50 mm across is microfabricated with pairs of electrodes on the top and bottom.…”

Section: Introductionmentioning

confidence: 99%

“…A small channel, typically 50 mm across is microfabricated with pairs of electrodes on the top and bottom. Using negative dielectrophoresis, particles are repelled from the electrode edges and form a tight beam in the channel centre [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…As has been shown [11][12][13], it is relatively easy to focus or trap large particles such as cells in microchannels using relatively low voltages. However, in order to achieve effective focusing of nanoparticles, the magnitude of the DEP force, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…DEP has already successfully been employed to the manipulation of micro-and nanoparticles, for cell sorting and separation [9][10][11][12][13][14][15][16][17] single cell characterisation [15,[18][19][20] …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

3D focusing of nanoparticles in microfluidic channels

Morgan

Holmes

Green

2003

IEE Proc., Nanobiotechnol.

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Dynamic focusing of particles can be used to centre particles in a fluid stream, ensuring the passage of the particles through a specified detection volume. This paper describes a method for focusing nanoparticles using dielectrophoresis. The method differs from other focusing methods in that it manipulates the particles and not the fluid. Experimental focusing is demonstrated for a range of different particle types, and discussed in terms of the operational limits of the device. Dynamic numerical simulations of the particle motion in the device are presented and compared with the experimental results. The potential of the device for nanoparticle control and manipulation in microfluidic chips is discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

3D focusing of nanoparticles in microfluidic channels

Morgan

Holmes

Green

2003

IEE Proc., Nanobiotechnol.

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“…Dielectrophoresis (DEP) -the action of polarizable particles in non-uniform electric fields -can be used to produce stable traps for bioparticles [1][2][3] as well as provide cell separation based upon size or electrical properties [4,5].…”

Section: Introductionmentioning

confidence: 99%

A Dielectrophoresis-based Array Cytometer

Voldman

Toner

Gray

et al. 2001

Transducers ’01 Eurosensors XV

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SUMMARYWe present the initial results of a cytometer that uses an array of extruded high-performance dielectrophoretic traps to capture, hold, and selectively release multiple single bioparticles. We have fabricated small arrays of traps and demonstrated single-bead and single-cell trapping, electrically switched bead release from single traps, fluorescence microscopy of trapped viable single cells, and quantitatively predicted trap strength.

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Dielectrophoretic manipulation of suspended submicron particles

et al. 2000

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Planar and three‐dimensional multi‐electrode systems with dimensions of 2 — 40 μm were fabricated by IC technology and used for trapping and aggregation of microparticles. To achieve negative dielectrophoresis (repelling forces) in aqueous solution, radiofrequency (RF) electric fields were used. Experimentally, particles down to 100 nm in diameter were enriched and trapped as aggregates in field cages and dielectrophoretic microfilters and observed using confocal fluorimetry. Theoretically, single particles with an effective diameter down to about 35 nm should be trappable in micron field cages. Due to the unavoidable Ohmic heating, RF electric fields can induce liquid streaming in extremely small channels (12 μm in height). This can be used for pumping and particle enrichment but it enhances Brownian motion and counteracts dielectrophoretic trapping. Combining Brownian motion with ratchet‐like dielectrophoretic forces enables the creation of Brownian pumps that could be used as sensitive separation devices for submicron particles if liquid pumping is avoided in smaller structures.

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A 3-D microelectrode system for handling and caging single cells and particles

Cited by 233 publications

References 24 publications

3D focusing of nanoparticles in microfluidic channels

3D focusing of nanoparticles in microfluidic channels

A Dielectrophoresis-based Array Cytometer

Dielectrophoretic manipulation of suspended submicron particles

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