A 25-kDa Protein Is Associated with the Envelopes of Occluded Baculovirus Virions

Russell, Rebecca L. Q.; Rohrmann, George F.

doi:10.1006/viro.1993.1404

Cited by 59 publications

(49 citation statements)

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“…4 j-o). Furthermore, previous studies have shown that the ODV-associated proteins accumulate at the periphery of the virogenic stroma (Russell & Rohrmann, 1993, 1997Hong et al, 1997). Taken together, these two independent observations lead to speculation that IE1 associates with the virogenic stroma.…”

Section: Two Odv-associated Proteins P91-gfp and Gfp-odv-e25 Become mentioning

confidence: 72%

“…ODV-E25, ODV-E66, P91 and P74) were shown to localize to the periphery of the nucleus (Russell & Rohrmann, 1997;Braunagel et al, 1999;RosasAcosta et al, 2001;Slack et al, 2001). More detailed observations with immunoelectron microscopy, however, have revealed that these proteins are mainly localized to the peristromal region in association with ODVs and intranuclear microvesicles (Russell & Rohrmann, 1993, 1997Braunagel et al, 1996a, b;Hong et al, 1997). The intranuclear microvesicles are the proposed source of ODV envelopes (Hong et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

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IE1, a principal transcriptional activator of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), is an essential factor for viral DNA replication. During viral infection, IE1 accumulates in discrete subnuclear structures where viral DNA replication occurs. To analyse the dynamic properties of IE1, we monitored green fluorescent protein-tagged IE1 (IE1-GFP) in BmNPV-infected B. mori cells by live-cell microscopy. Time-lapse imaging showed that IE1-associated structures gradually expanded and occasionally fused with one another, while photobleaching experiments revealed that IE1-GFP was relatively immobile inside the IE1-associated structures. To investigate the spatial relationships between IE1 and viral structural proteins in infected cells, three GFP-tagged viral components were expressed together with DsRed-tagged IE1. Two structural proteins that constitute the occlusion-derived virus (ODV), P91-GFP and GFP-ODV-E25, localized to the periphery of the IE1-associated structures. While local accumulations of these proteins were often in contact with the IE1-associated structures, they did not extend beyond the boundaries of the structures. In contrast, the major capsid protein VP39-GFP predominantly accumulated within the IE1-associated structures. These data indicated, in conjunction with the finding of a high DNA content in the structures, that IE1 localizes to the virogenic stroma and therefore support the prediction previously proposed that the virogenic stroma is a site for viral DNA replication as well as for the assembly of nucleocapsids.

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Section: Two Odv-associated Proteins P91-gfp and Gfp-odv-e25 Become mentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

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“…The very small quantity of p74 present in virion envelopes has rendered this polypeptide undetectable by standard methods, and raises the issue of how many proteins are actually associated with the envelope. Major species recently identified with the PDV envelope include a 25 kDa species (Russell & Rohrmann, 1993), a 66 kDa and a 43 kDa species (Braunagel & Summers, 1994). However, p74 is the first PDV envelope protein demonstrated to have a functional significance in primary infection.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, PDV gains entry to insect epithelial cells by fusion at the cell surface (Granados & Lawler, 1981). Spikes that could participate in adsorption, fusion and penetration have not been identified on envelopes of PDV (see Rohrmann, 1992), but many polypeptides of unknown function [p25, PDV-E66, ODV-E56 (ODV-6E), ODV-E18 and ODV-E35] have been identified as constituents of the PDV envelope (Russell & Rohrmann, 1993 ;Braunagel & Summers, 1994 ;Hong et al, 1994 ;Braunagel et al, 1996 a, b ;Theilmann et al, 1996). A major glycoprotein of PDV (gp41) was found to localize to the tegument region of the virion (Whitford & Faulkner, 1992 a, b), and although probably not involved in virus attachment or fusion, it may play a role in capsid entry or transport once fusion occurs.…”

Section: Introductionmentioning

confidence: 99%

Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo.

Faulkner

Kuzio

Williams

et al. 1997

Journal of General Virology

141

122

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In nature, nuclear polyhedrosis viruses (NPV) are transmitted when susceptible insect larvae ingest viral occlusion bodies (OB). These dissociate in the alkaline environment of the midgut and release encapsulated virions (PDV) which bind to midgut epithelial cells and initiate an infection. A previous study showed that expression of the Autographa californica NPV (AcMNPV) p74 gene during replication is essential for the production of infectious OB. A set of p74 deletion and overexpression recombinants was used for the production and screening of monoclonal antibodies, and for an investigation of gross cytopathology and localization of p74. No differences in virus structure or morphogenesis were observed in infected cells when the p74 gene of AcMNPV was deleted, even though the infectivity of OB harvested from the cells was abolished when they were fed to Trichoplusia ni

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“…Among these structural proteins, at least eight have been known to be specific to the ODV phenotype. These are as follows: VP17 (Funk and Consigli, 1993), ODV-E25 (Russell and Rohrmann, 1993), ODV-E35 (Braunagel et al, 1996), GP41 (Whitford and Faulkner, 1993), P74 (Kuzio et al, 1089), ODV-E18 (Braunagel et al, 1996), ODV-E66 (Hong et al, 1994), and ODVP-6E/ODV-E56 (Braunagel et al, 1996, Theilmann et al, 1996. The proteins that could participate in adsorption, fusion, and penetration have not been identified.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a Conserved Baculoviral Structural Protein ODVP-6E/ODV-E56 from Choristoneura fumiferana Granulovirus

Rashidan¹,

Nassoury²,

Giannopoulos³

et al. 2002

BMB Reports

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A 25-kDa Protein Is Associated with the Envelopes of Occluded Baculovirus Virions

Cited by 59 publications

References 0 publications

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo.

Identification and Characterization of a Conserved Baculoviral Structural Protein ODVP-6E/ODV-E56 from Choristoneura fumiferana Granulovirus

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