A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere

Lechner, Johannes; Carbon, John

doi:10.1016/0092-8674(91)90501-o

Cited by 306 publications

(323 citation statements)

References 42 publications

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“…Two essential components of the S. cerevisiae kinetochore, Cbf2p/Ndc10p and Ctf13p, have been shown to be phosphorylated in vitro or as recombinant proteins in insect cells, respectively (Jiang et al 1995;Kaplan et al 1997). Also, treatment of yeast kinetochore proteins with phosphatase in vitro prevents binding to centromere sequences (Lechner and Carbon 1991). Therefore, the phosphorylation state of kinetochore complexes may affect CEN DNA binding.…”

Section: Discussionmentioning

confidence: 99%

Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint

Bloecher

Tatchell²

1999

Genes & Development

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Section: Discussionmentioning

confidence: 99%

Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint

Bloecher

Tatchell²

1999

Genes & Development

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“…In addition cooperative DNA binding of other CBF3 components may be required. Some of these interactions should be unspecific as was concluded from the finding that CBF3-CEN DNA complexes include 30 bp of DNA to the right of CDE III that exhibit no sequence similarities among different S. cerevisiae CEN DNAs [9]. Cbf3a is the only CBF3 component that shows unspecific DNA binding in vitro (Stemmann and Lechner, unpublished) and therefore may be responsible for the unspecific protein-DNA interaction within the CBF3-CEN DNA complex.…”

Section: Cbf3-cen Dna Complex Structurementioning

confidence: 99%

The Saccharomyces cerevisiae kinetochore

Lechner¹,

Ortiz²

1996

FEBS Letters

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Accurate chromosome segregation is dependent on a specialized chromosomal structure, the kinetochore/centromere. The only essential constituent of the S. cerevisiae kinetochore established today is CBF3, a multisubunit complex that binds to S. cerevisiae centromere DNA. Therefore CBF3 and its four components, Cbf3a, Cbf3b, Cbf3c and Cbf3d, will form the centerpiece of this review. In addition, we will describe proteins that are putatively involved in kinetochore function specifically in the context with CBF3 interaction. Furthermore, we discuss the role of the S. cerevisiae kinetochores in a putative cell cycle checkpoint control and in microtubule attachment. Key words:Centromere; Kinetochore; CBF3; Saccharomyces cerevisiae Recent reviews that describe the S. cerevisiae kinetochore are available [1,5,6]. This review focuses on the protein components of the S. cerevisiae kinetochore. First we describe proteins that physically interact with the CEN DNA and we emphasize the analysis of CBF3 (_centromere DNA binding factor), a key component of the S. cerevisiae kinetochore. Second we describe proteins that are putatively involved in kinetochore function (as structural components or regulators) as deduced from genetic interactions with CEN DNA or CEN DNA binding proteins. Finally we discuss the role of the S. cerevisiae kinetochore as a putative cell cycle checkpoint and in microtubule interaction. CEN DNA binding proteins

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“…Although each region plays an important role in kinetochore function, mutational analyses suggest that only the CDEIII region is essential for kinetochore function (Hegemann et al, 1988). Biochemical purification of proteins that bind CDEIII and subsequent genetic analyses identified the CBF3 complex, which is comprised of three proteins: Ctf13p (p58), Cep3p (p64), and Ndc10p (p110) (Lechner and Carbon, 1991;Doheny et al, 1993;Jiang et al, 1993;Lechner, 1994;Strunnikov et al, 1995). Additional genes critical for the proper function of CBF3 were identified in dosage suppression studies; SKP1 was found as a dosage suppressor of a mutation in CTF13 (ctf13-30); similarly, SGT1 was found as a dosage suppressor of a mutation in SKP1 (skp1-4) (Connelly and Hieter, 1996;Kitagawa et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Sgt1p and Skp1p Modulate the Assembly and Turnover of CBF3 Complexes Required for Proper Kinetochore Function

Rodrigo-Brenni

Thomas

Bouck

et al. 2004

MBoC

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Kinetochores are composed of a large number of protein complexes that must be properly assembled on DNA to attach chromosomes to the mitotic spindle and to coordinate their segregation with the advance of the cell cycle. CBF3 is an inner kinetochore complex in the budding yeast Saccharomyces cerevisiae that nucleates the recruitment of all other kinetochore proteins to centromeric DNA. Skp1p and Sgt1p act through the core CBF3 subunit, Ctf13p, and are required for CBF3 to associate with centromeric DNA. To investigate the contribution of Skp1p and Sgt1p to CBF3 function, we have used a combination of in vitro binding assays and a unique protocol for synchronizing the assembly of kinetochores in cells. We have found that the interaction between Skp1p and Sgt1p is critical for the assembly of CBF3 complexes. CBF3 assembly is not restricted during the cell cycle and occurs in discrete steps; Skp1p and Sgt1p contribute to a final, rate-limiting step in assembly, the binding of the core CBF3 subunit Ctf13p to Ndc10p. The assembly of CBF3 is opposed by its turnover and disruption of this balance compromises kinetochore function without affecting kinetochore formation on centromeric DNA.

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A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere

Cited by 306 publications

References 42 publications

Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint

Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint

The Saccharomyces cerevisiae kinetochore

Sgt1p and Skp1p Modulate the Assembly and Turnover of CBF3 Complexes Required for Proper Kinetochore Function

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