A 24,482-bp deletion is associated with increased seed weight in Brassica napus L.

Zhang, Xiaohui; Huang, Qiyang; Wang, Pengfei; Liu, Feiyang; Luo, Mudan; Li, Xiang; Wang, Zhuanrong; Wan, Lili; Yang, Guangsheng; Hong, Dengfeng

doi:10.1007/s00122-021-03850-x

Cited by 5 publications

(4 citation statements)

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“…In our previous study, we found that HZ396 is a natural line with a 24 482 bp deletion containing BnaC09G0551500ZS and BnaC09G0551600ZS (the two genes were combined to be a functional gene) and we named it BnaJUL1 in rapeseed (Zhang et al, 2021). We also noted that HZ396 and NIL(Y106) plants have different responses to ABA concentration gradients (Figure ), suggesting that the BnaJUL1 genes are related to ABA response.…”

Section: Resultsmentioning

confidence: 99%

“…We used the rapeseed inbred line Y127 and constructed the transgenic variations BnaJUL1‐cri and BnaTBCC1‐cri in our laboratory. HZ396 was an inbred line with high thousand‐seed weight and NIL(Y106) was the near isogenic line of HZ396 (Li et al, 2015; Zhang et al, 2021). Plants were maintained in a light culture room of our laboratory at 22°C under conditions of 70% humidity and a 16 h light/8 h dark photoperiod.…”

Section: Methodsmentioning

confidence: 99%

“…The homologous genes of JUL1 in rapeseed (BnaJUL1) encodes this E3 ligase. In our previous research, we found an obvious difference in the ABA response to germination rate between the HZ396 line of rapeseed, which contains a 24,482bp deletion that includes BnaJUL1, and the NIL(Y106) line (Zhang et al, 2021). Whether BnaJUL1 regulates the molecular mechanism underlying drought tolerance in rapeseed, is worth investigating.…”

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confidence: 96%

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RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

Hu,

Luo,

Zhou

et al. 2023

Plant Cell & Environment

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Drought stress poses a persistent threat to field crops and significantly limits global agricultural productivity. Plants employ ubiquitin‐dependent degradation as a crucial post‐translational regulatory mechanism to swiftly adapt to changing environmental conditions. JUL1 is a RING‐type E3 ligase related to drought stress in Arabidopsis. In this study, we explored the function of BnaJUL1 (a homologous gene of JUL1 in Brassica napus) and discovered a novel gene BnaTBCC1 participating in drought tolerance. First, we utilised BnaJUL1‐cri materials through the clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated protein 9 system. Second, we confirmed that BnaJUL1 regulated drought tolerance through the drought tolerance assay and transcriptome analysis. Then, we identified a series of proteins interacting with BnaJUL1 through yeast library screening, including BnaTBCC1 (a tubulin binding cofactor C domain‐containing protein); whose homologous gene TBCC1 knockdown mutants (tbcc1‐1) exhibited ABA‐sensitive germination in Arabidopsis, we then confirmed the involvement of BnaTBCC1 in drought tolerance in both Arabidopsis and Brassica. Finally, we established that BnaJUL1 could ubiquitinate and degrade BnaTBCC1 to regulate drought tolerance. Consequently, our study unveils BnaJUL1 as a novel regulator that ubiquitinates and degrades BnaTBCC1 to modulate drought tolerance and provided desirable germplasm for further breeding of drought tolerance in rapeseed.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 96%

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RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

Hu,

Luo,

Zhou

et al. 2023

Plant Cell & Environment

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“…The seed coat patterns determined by the palisade cells differ greatly among different brassica species [ 12 ]. Under scanning electron microscopy (SEM), the seed coat cell patterns can be recognized and used to measure cell size [ 13 ]. However, certain challenges hinder the application of this method, i.e., the seed margins of some materials are difficult to distinguish, and SEM assays are labor intensive and costly.…”

Section: Introductionmentioning

confidence: 99%

A simple and efficient method to quantify the cell parameters of the seed coat, embryo and silique wall in rapeseed

et al. 2022

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Background Researchers interested in the seed size of rapeseed need to quantify the cell size and number of cells in the seed coat, embryo and silique wall. Scanning electron microscope-based methods have been demonstrated to be feasible but laborious and costly. After image preparation, the cell parameters are generally evaluated manually, which is time consuming and a major bottleneck for large-scale analysis. Recently, two machine learning-based algorithms, Trainable Weka Segmentation (TWS) and Cellpose, were released to overcome this long-standing problem. Moreover, the MorphoLibJ and LabelsToROIs plugins in Fiji provide user-friendly tools to deal with cell segmentation files. We attempted to verify the practicability and efficiency of these advanced tools for various types of cells in rapeseed. Results We simplified the current image preparation procedure by skipping the fixation step and demonstrated the feasibility of the simplified procedure. We developed three methods to automatically process multicellular images of various tissues in rapeseed. The TWS–Fiji (TF) method combines cell detection with TWS and cell measurement with Fiji, enabling the accurate quantification of seed coat cells. The Cellpose–Fiji (CF) method, based on cell segmentation with Cellpose and quantification with Fiji, achieves good performance but exhibits systematic error. By removing border labels with MorphoLibJ and detecting regions of interest (ROIs) with LabelsToROIs, the Cellpose–MorphoLibJ–LabelsToROIs (CML) method achieves human-level performance on bright-field images of seed coat cells. Intriguingly, the CML method needs very little manual calibration, a property that makes it suitable for massive-scale image processing. Through a large-scale quantitative evaluation of seed coat cells, we demonstrated the robustness and high efficiency of the CML method at both the single-cell level and the sample level. Furthermore, we extended the application of the CML method to developing seed coat, embryo and silique wall cells and acquired highly precise and reliable results, indicating the versatility of this method for use in multiple scenarios. Conclusions The CML method is highly accurate and free of the need for manual correction. Hence, it can be applied for the low-cost, high-throughput quantification of diverse cell types in rapeseed with high efficiency. We envision that this method will facilitate the functional genomics and microphenomics studies of rapeseed and other crops.

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Fertility, genome stability, and homozygosity in a diverse set of resynthesized rapeseed lines

Katche

Schierholt²,

Becker³

et al. 2023

The Crop Journal

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A 24,482-bp deletion is associated with increased seed weight in Brassica napus L.

Cited by 5 publications

References 122 publications

RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

A simple and efficient method to quantify the cell parameters of the seed coat, embryo and silique wall in rapeseed

Fertility, genome stability, and homozygosity in a diverse set of resynthesized rapeseed lines

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