A 2-sulfated, 3-linked α-l-galactan is an anticoagulant polysaccharide

Characterization of Sulfate Groups and Assessment of Anti-Coagulant Activity of Glucomannan Sulfate Prepared from Konjac Glucomannan

“…The results show that APTT is powerfully prolonged by KOGMS at 0.5mgmL −1 . Usually, prolongation of APTT indicates inhibition of the intrinsic or common pathway [15]. In the present study, KOGMS inhibited the intrinsic or common pathways of coagulation.…”

Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed Gracilaria ornata

“…Pereira et al (2005) reported the occurrence of -4-α-Galp-(1-3)-β-Galp1, but with a variable sulfation pattern, having 2,3-di-sulfated and 2-sulfated, for the red alga G. crinale. The presence of 2-sulfated, 3-linked α-Lgalactan, was also characteristics of some marine invertebrates (Pereira et al 2002). More recently, Aquino et al (2005) identified sulfated galactans from the marine angiosperms (Ruppia maritima, Halodule wrightii, and Halophila decipiens).…”

“…SPs from different algal species have demonstrated that each polysaccharide possesses a particular biological action, and as a consequence of the presence of sulfate radicals, these polymers deserve to be evaluated using different biological assays (Mourão and Pereira 1999). The occurrence of -4-α-Galp-(1-3)-β-Galp1, but with a variable sulfation pattern, having 2,3-disulfated and 2-sulfated, has been attributed as a structural requirement for the anticoagulant action of galactans from red algae (Pereira et al 2005), and as a structure-anticoagulant relationship that also indicate 2-sulfated, 3-linked α-L-galactan, being a potent thrombin inhibitor mediated by antithrombin or heparin cofactor II, for marine invertebrates (Pereira et al 2002). More recently, Fonseca et al (2008) compared a SP isolated from B. occidentalis having anticoagulant activity (90 IU mg -1 ) with that of G. crinale (65 IU mg -1 ) (Pereira et al 2005) (Rhodophyta) on specific coagulation assays and experimental models of thrombosis.…”

Effect of Molecular Mass on Antitumor Activity of Heteropolysaccharide fromPoria cocos

“…The bioactivity of polysaccharides is related to their molecular mass, degree of substitution, degree of branching, conformation in solution, and sugar component. 6,7) So fractions of the polysaccharides having different molecular mass are often needed to investigate physical properties and bioactivity. Size exclusion chromatography (SEC) is presently the most popular method for fractionation and characterization of both synthetic and natural polymers.…”