A 2,3‐butanediol dehydrogenase from Paenibacillus polymyxa<scp>ZJ</scp>‐9 for mainly producing R,R‐2,3‐butanediol: Purification, characterization and cloning

Gao, Jian; Yang, Huanhuan; Feng, Xiaohai; Li, Sha; Xu, Hong

doi:10.1002/jobm.201200152

Cited by 33 publications

(21 citation statements)

References 20 publications

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“…Characterization and gene cloning of 2,3‐BDO dehydrogenase by Paenibacillus polymyxa ZJ‐9 showed that this enzyme is a new type of BDH, significantly different than other reported BDHs. It acts more like a reductase of R ‐acetoin rather than a dehydrogenase 34. R ‐BDH from P. polymyxa ATCC 12321 was functionally characterized and classified in a medium‐chain dehydrogenase/reductase superfamily, differently from meso ‐ and S ‐BDH, which belong to the short‐chain dehydrogenase/reductase superfamily 35.…”

Section: Microbial Production Metabolismmentioning

confidence: 99%

Bioprocess Development for 2,3‐Butanediol Production by Paenibacillus Strains

et al. 2021

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This review focuses on the fermentation potential of GRAS (generally recognized as safe) bacteria that belong to Paenibacillus species on the production of 2,3‐butanediol (2,3‐BDO). P. polymyxa, P. peoriae, and P. brasilensis were reported as highly efficient microbial producers of 2,3‐BDO and acetoin. The factors contributing to the fermentative production of 2,3‐BDO are addressed with particular emphasis on crucial fermentation parameters, precursors that increase fermentation efficiency, and the significance of metabolic pathways. Several carbon sources from renewable resources can be consumed by Paenibacillus strains, which favors the development of alternative biorefinery concepts including the production of 2,3‐BDO within a circular bio‐economy approach. Downstream separation and purification processes used for the recovery of bio‐based 2,3‐BDO are also discussed. The production of 2,3‐BDO isomers with high stereoisomeric purity can lead to new applications and markets. Paenibacillus strains could be a viable alternative to biosafety level 2 strains that were widely investigated for the production of 2,3‐BDO.

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Section: Microbial Production Metabolismmentioning

confidence: 99%

Bioprocess Development for 2,3‐Butanediol Production by Paenibacillus Strains

et al. 2021

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“…Because operational parameters vary for different environment‐medium‐microorganism, the optimal value should be established individually for each strain and substrate used. More recently, some studies have focused on the genetic modification of yeasts and bacteria toward the production of 2,3‐BD, but the reported results of productivity and yields of this chemical are often low when compared to values obtained using wild‐type strains …”

Section: Introductionmentioning

confidence: 99%

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Cortivo

Machado

Hickert

et al. 2019

Biotechnology Progress

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We investigated the production of 2,3-butanediol by two enterobacteria isolated from an environmental consortium, Klebsiella pneumoniae BLh-1 and Pantoea agglomerans BL1, in a bioprocess using acid and enzymatic hydrolysates of soybean hull as substrates. Cultivations were carried out in orbital shaker under microaerophilic conditions, at 30 C and 37 C, for both bacteria. Both hydrolysates presented high osmotic pressures, around 2,000 mOsm/kg, with varying concentrations of glucose, xylose, and arabinose. Both bacteria were able to grow in the hydrolysates, at both temperatures, and they efficiently converted sugars into 2,3-butanediol, showing yields varying from 0.25 to 0.51 g/g of sugars and maximum 2,3-butanediol concentrations varying from 6.4 to 21.9 g/L. Other metabolic products were also obtained in lower amounts, notably ethanol, which peaked at 3.6 g/L in cultures using the enzymatic hydrolysate at 30 C. These results suggest the potential use of these recently isolated bacteria to convert lignocellulosic biomass hydrolysates into value-added products. K E Y W O R D S 2,3-butanediol, bioprocess, Klebsiella pneumoniae, lignocellulosic biomass hydrolysates, Pantoea agglomerans, soybean hull

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“…D -2,3-butanediol as one of the promising bulk chemicals has extensive applications in cosmetics, foods, transport fuels, medicines, and polymers industries [ 1 ]. In general, 2,3-butanediol exists in three stereoisomeric forms: D -2,3-butanediol, L -2,3-butanediol and meso -2,3-butanediol [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…All these isomers are valuable chemicals that provide chiral groups in drugs [ 3 ]. D- 2,3-butanediol is also used as an antifreeze agent because of its low freezing point (-60°C) [ 1 ]. The production of 2,3-butanediol with high optical purities is therefore highly desirable [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Deletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis

Kang

et al. 2014

Biotechnol Biofuels

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BackgroundD-2,3-butanediol has many industrial applications such as chiral reagents, solvents, anti-freeze agents, and low freezing point fuels. Traditional D-2,3-butanediol producing microorganisms, such as Klebsiella pneumonia and K. xoytoca, are pathogenic and not capable of producing D-2,3-butanediol at high optical purity. Bacillus licheniformis is a potential 2,3-butanediol producer but the wild type strain (WX-02) produces a mix of D- and meso-type isomers. BudC in B. licheniformis is annotated as 2,3-butanediol dehydrogenase or acetoin reductase, but no pervious experiment was performed to verify this hypothesis.ResultsWe developed a genetically modified strain of B. licheniformis (WX-02 ΔbudC) as a D-2,3-butanediol producer with high optimal purity. A marker-less gene deletion protocol based on a temperature sensitive knock-out plasmid T2-Ori was used to knock out the budC gene in B. licheniformis WX-02. The budC knock-out strain successfully abolished meso-2,3-butanediol production with enhanced D-2,3-butanediol production. No meso-BDH activity was detectable in cells of this strain. On the other hand, the complementary strain restored the characteristics of wild strain, and produced meso-2,3-butanediol and possessed meso-BDH activity. All of these data suggested that budC encoded the major meso-BDH catalyzing the reversible reaction from acetoin to meso-2,3-butanediol in B. licheniformis. The budC knock-out strain produced D-2,3-butanediol isomer only with a high yield of 30.76 g/L and a productivity of 1.28 g/L-h.ConclusionsWe confirmed the hypothesis that budC gene is responsible to reversibly transfer acetoin to meso-2,3-butanediol in B. licheniformis. A mutant strain of B. licheniformis with depleted budC gene was successfully developed and produced high level of the D-2,3-butanediol with high optimal purity.

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A 2,3‐butanediol dehydrogenase from Paenibacillus polymyxaZJ‐9 for mainly producing R,R‐2,3‐butanediol: Purification, characterization and cloning

Cited by 33 publications

References 20 publications

Bioprocess Development for 2,3‐Butanediol Production by Paenibacillus Strains

Bioprocess Development for 2,3‐Butanediol Production by Paenibacillus Strains

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Deletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis

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