A-16686, a new antibiotic from Actinoplanes I. Fermentation, isolation and preliminary physico-chemical characteristics.

Abstract

Actinoplanes sp. ATCC 33076 is a new strain that was found to produce an antibiotic, designated A-16686, which is a complex of three closely-related polypeptides containing chlorinated phenyl moieties and D-mannose. Both the complex and the single fractions possess a good activity against Gram-positive bacteria. A-16686 specifically inhibits the synthesis of the bacterial cell wall. Culture media* Medium No. 2 (yeast extract-malt agar)

A crystal structure of a dimer of the antibiotic ramoplanin illustrates membrane positioning and a potential Lipid II docking interface

“…The ramoplanins are a family of closely related glycolipodepsipeptide antibiotics secreted by Actinoplanes ATCC 33076 (5,6). At least three forms are known, A1, A2, and A3; these forms differ in their acyl chain substituents, but possess essentially identical antibiotic activities.…”

Complexation of peptidoglycan intermediates by the lipoglycodepsipeptide antibiotic ramoplanin: Minimal structural requirements for intermolecular complexation and fibril formation

“…1) is a promising candidate to replace vancomycin. Produced by fermentation of Actinoplanes ATCC 33076, ramoplanin A2 is highly active against numerous Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) (2-6), vancomycin-resistant Enterococcus faecium (VRE), and those resistant to ampicillin and erythromycin (6)(7)(8)(9)(10)(11). Ramoplanin factor A2 is currently under Phase III clinical development for eradication of VRE and MRSA.…”

Inhibition of peptidoglycan biosynthesis by ramoplanin

“…Ramoplanin is a new lipoglycopeptide antibiotic produced from an Actinoplanes strain (4). It is a complex of three components, designated Al, A2, and A3.…”