A 15-Plex/xMAP Method to Detect 15 Animal Ingredients by Suspension Array System Coupled with Multifluorescent Magnetic Beads

Abstract

A 15-plex/xMAP method was established to simultaneously detect 15 animal ingredients. The method, based on multiplex PCR and the Luminex multifluorescent bead detection system, was able to sensitively differentiate mammal and ruminant species, and in particular, to detect 11 mammal and two poultry species. The estimated sensitivity of a mixed-meat sample was 0.1% (weight percentage). In a survey of commercially processed meat products, nine of 16 samples were found to contain adulterated ingredients. The assay of the 16 samples took only 3 h for the identification of the 15 animal ingredients.

Establishment and application of a 10‐plex liquid bead array for the simultaneous rapid detection of animal species

“…At present, a commercial qualitative LCD array kit (Meat 5.0, Chipron GmbH, Berlin, Germany) can identify 17 meat and seven poultry species, which include cattle, sheep, equine, goat, camel, buffalo, pork, kangaroo, hare, rabbit, reindeer, roe deer, red deer, fallow deer, springbok, canine, cat, chicken, turkey, goose, ostrich, mallard duck, Muscovy duck and pheasant. In addition, a 15‐plex xMAP was reported to identify mammal (horse, donkey, camel, sheep, swine, red deer, buffalo, yak, dog, rat, mouse, cattle, rabbit, cat and raccoon dog), ruminants (camel, sheep, elk, buffalo and yak) and other individual species including horse, donkey, camel, sheep, swine, red deer, buffalo, yak, dog, rat, mouse, chicken and Beijing duck . Compared to these two methods, the present study adds mink and fox, which are raised for the production of fur with unrestricted doses of drugs and are reported to be substituted by some dishonest traders .…”

“…In recent years, this method has been successfully used to identify bacteria, viruses, parasites, toxins and food allergens . However, there have been few reports of this method being used for the detection of animal species …”

Real-time qPCR for the detection of puffer fish components from Lagocephalus in food: L. inermis, L. lagocephalus, L. gloveri, L. lunaris, and L. spadiceus

“…Omics-based methods have become a comprehensive solution for food fraud (15,16). PCR-based methods for the detection and differentiation of species have usually been applied due to their high specificity, sensitivity, and speed, including qPCR (17), digital PCR (18,19), gene chip (20), and DNA barcode (21) which can quickly distinguish all animals and plants raw materials used in food and has attracted international attention and developed rapidly. TaqMan-based real-time quantitative polymerase chain reaction (qPCR) plays an important role in food authenticity identification.…”