A 13-Kilodalton Maize Mitochondrial Protein in
            <i>E. coli</i>
            Confers Sensitivity to
            <i>Bipolaris maydis</i>
            Toxin

Dewey, Ralph E.; Siedow, James N.; Timothy, D. H.; Levings, Charles S.

doi:10.1126/science.3276005

Cited by 182 publications

(112 citation statements)

References 20 publications

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“…feature is distinct from that of the common bean (Phaseolus vulgaris) CMS gene orf239, which results in sporophytic male sterility in transgenic tobacco plants (He et al, 1996). It was previously shown that the maize CMS-T gene urf13 also encodes a cytotoxic peptide (Dewey et al, 1988). In addition, the expression of CMS-associated genes orf552 from sunflower (Helianthus annuus) and orf138 from radish were also lethal to E. coli (Nakai et al, 1995;Duroc et al, 2005;Y.-G. Liu, unpublished data).…”

Section: Discussion Abnormal Mitochondrial Orfs and Cmsmentioning

confidence: 99%

Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing

et al. 2006

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Cytoplasmic male sterility (CMS) and nucleus-controlled fertility restoration are widespread plant reproductive features that provide useful tools to exploit heterosis in crops. However, the molecular mechanism underlying this kind of cytoplasmicnuclear interaction remains unclear. Here, we show in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene and encodes a cytotoxic peptide. Expression of orf79 in CMS lines and transgenic rice plants caused gametophytic male sterility. Immunoblot analysis showed that the ORF79 protein accumulates specifically in microspores. Two fertility restorer genes, Rf1a and Rf1b, were identified at the classical locus Rf-1 as members of a multigene cluster that encode pentatricopeptide repeat proteins. RF1A and RF1B are both targeted to mitochondria and can restore male fertility by blocking ORF79 production via endonucleolytic cleavage (RF1A) or degradation (RF1B) of dicistronic B-atp6/orf79 mRNA. In the presence of both restorers, RF1A was epistatic over RF1B in the mRNA processing. We have also shown that RF1A plays an additional role in promoting the editing of atp6 mRNAs, independent of its cleavage function.

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Section: Discussion Abnormal Mitochondrial Orfs and Cmsmentioning

confidence: 99%

Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing

et al. 2006

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“…These properties help to explain its known biological activity. T-URFl 3 confers sensitivity to fungal toxins (BmT and Pm) and to methomyl when the gene coding for it is expressed in E. coli (5,10), and also confers sensitivity on yeast cells, provided that it is targeted to mitochondria (20). Both toxins and methomyl dissipate the membrane potential of mitochondria and cause leakage of ions.…”

Section: Discussionmentioning

confidence: 99%

“…The grids, with section side down, were floated on drops of the solutions placed on the Parafilm. The sections were preincubated for 10 …”

Section: Immunolabelingmentioning

confidence: 99%

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T-URF 13 Protein from Mitochondria of Texas Male-Sterile Maize (Zea mays L.)

Hack¹,

Lin²,

Yang³

et al. 1991

Plant Physiol.

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The protein T-URF13 (URF13) is specific to mitochondria of maize (Zea mays L.) with Texas (T) male-sterile cytoplasm and has been implicated in causing male sterility and susceptibility to T-cytoplasm-specific fungal diseases. T-URF13 was purified from isolated mitochondria from maize (line B73) with T cytoplasm by gel filtration and a quasi two-dimensional polyacrylamide gel electrophoresis system. Antibodies to the purified and denatured protein were produced in rabbits. Anti-T-URF13 antiserum was used to show that T-URF13 is in the inner membrane of mitochondria and behaves as an integral membrane protein when mitochondria are fractionated with sodium carbonate or Triton X-114. The antiserum and protein A tagged with 20-nanometer-gold particles were used to localize T-URF13 in T mitochondria by electron microscopy of sections of isolated mitochondria from etiolated shoots and sections of roots and of tapetal cells at preand post-degeneration stages of microsporogenesis. The microscopic study confirms that T-URF13 is specifically localized in the mitochondrial membranes of all of the T mitochondria tested, notably those in the tapetum from the meiocyte stage to the latemicrospore stage. No change in the amount of labeled T-URF13 protein in the mitochondria of aging tapetal cells was detected.Maize (Zea mays L.) lines with T4 cytoplasm are completely male sterile and were widely used for producing hybrid seeds

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“…The most extensively analyzed CMS system is probably the CMS-T maize. In CMS-T, mitochondrial chimeric gene T-urf13 encodes a 13 kDa polypeptide (URF13) which strongly inhibits E. coli growth [6]. A few other CMS-associated genes (sunflower orf522, Brassica Ogura radish orf138 and BT-rice orf79) have also been shown to encode the toxic protein [7]- [9].…”

Section: Cms-associated Chimeric Orfs Are Often Toxicmentioning

confidence: 99%

The Cell Death in CMS Plants

Huang¹,

Zhang²,

Fang³

et al. 2011

IJBBB

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Abstract-Reactive oxygen species (ROS)-mediated PCD was found to occur in CMS lines of many species. The excess production of ROS is probably caused by the decrease of the activity of ROS-scavenging enzymes in CMS plants at the abortion peak. Why antioxidant enzyme activities decline? Mostly, the ascorbate-glutathione cycle of mitochondria is blocked by the depletion of ATP and NADH which would result from mitochondrial dysfunction induced by the expression of mitochondrial chimeric open-reading frames (ORFs). Moreover, the interaction between ROS and some signaling molecules, such as ethylene, attenuated the induction of the cyanide-resistant respiration, which exacerbates the accumulation of the ROS. Under oxidative stress, cellular protein especially membrane protein and DNA damage occur, and thus lead to the cell death. The characteristic feature of the programmed cell death (PCD) in CMS plants may be that it can be blocked by a matching nuclear pathway that restores normal regulation. We will discuss the central role of the mitochondrion in the whole pathways of the cell death in CMS plants.Index Terms-Cytoplasmic male sterility, Mitochondri-al, Programmed cell death.

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A 13-Kilodalton Maize Mitochondrial Protein in E. coli Confers Sensitivity to Bipolaris maydis Toxin

Cited by 182 publications

References 20 publications

Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing

Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing

T-URF 13 Protein from Mitochondria of Texas Male-Sterile Maize (Zea mays L.)

The Cell Death in CMS Plants

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