A 10-year single-center experience on Stenotrophomonas maltophilia resistotyping in Szeged, Hungary

Gajdács, Márió; Urbán, Edit

doi:10.1556/1886.2020.00006

Cited by 19 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S. maltophilia is an environmental globally emerging gram-negative multidrug-resistant organism that most commonly is associated with respiratory infections in human beings [2,10]. It is also responsible for many other infectious diseases, including bacteremia, endocarditis, and urethral infection, especially among the immunocompromised, as well as those undergoing aggressive treatments [1].…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

et al. 2020

View full text Add to dashboard Cite

Background Stenotrophomonas maltophilia (S. maltophilia) is an important opportunistic pathogen that can be isolated in hospitals. With the abuse of broad spectrum antibiotics and invasive surgical devices, the rate of S. maltophilia infection is increasing every year. This study was an epidemiological analysis of the clinical and molecular characteristics of S. maltophilia infection in a Chinese teaching hospital. The goal was to obtain a comprehensive understanding of the status of S. maltophilia infection to provide strong epidemiological data for the prevention and treatment of S. maltophilia infection. Results A total of 93 isolates from Renji Hospital affiliated with the Shanghai Jiaotong University School of Medicine were included, in which 62 isolates were from male patients. In addition, 81 isolates were isolated from sputum samples. A total of 86 patients had underlying diseases. All patients received antibiotics. Multilocus sequence typing (MLST) analysis indicated that 61 different sequence types (STs) were found (including 45 novel STs), and MLST did not show significantly dominant STs. Pulsed field gel electrophoresis (PFGE) results showed that 93 isolates could be divided into 73 clusters, and they also showed weak genetic linkages between isolates. The resistant rates to trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin were 9.7 and 4.3%, respectively, and all isolates were susceptible to minocycline. Four virulence gene’s loci Stmpr1, Stmpr2, Smf-1, and Smlt3773 were positive in 79.6, 91.4, 94.6, and 52.7% of the isolates, respectively. Three biofilm genes rmlA, spgM, and rpfF were positive in 82.8, 92.5, and 64.5% of the isolates, respectively. Mean biofilm forming level of OD492 was 0.54 ± 0.49. We did not find any significant difference between different genders and different age-groups. We retrospectively analyzed data from patients in the intensive care unit (ICU) and the control group. The independent risk factors of those who were infected in the ICU included immunosuppression and the increased antibiotic usage. Conclusions Most of the patients had prior medical usage histories and baseline diseases. The positive rate of virulence genes was high, the drug resistance rate of S. maltophilia was low, and the biofilm formation ability was strong. The increased use of antibiotics was an independent risk factor for S. maltophilia infection, which should receive more attention. No obvious clonal transmissions were found in the same departments.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Duan

Qin

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Stenotrophomonas maltophilia (S. maltophilia) is an important opportunistic pathogen that can be isolated in hospitals. With the abuse of broad spectrum antibiotics and invasive surgical devices, the rate of S. maltophilia infection is increasing every year. This study was an epidemiological analysis of the clinical and molecular characteristics of S. maltophilia infection in a Chinese teaching hospital. The goal was to obtain a comprehensive understanding of the status of S. maltophilia infection to provide strong epidemiological data for the prevention and treatment of S. maltophilia infection.Results: A total of 93 isolates from Renji Hospital affiliated with the Shanghai Jiaotong University School of Medicine were included, in which 62 isolates were from male patients. In addition, 81 isolates were isolated from sputum samples. A total of 86 patients had underlying diseases. All patients received antibiotics. Multilocus sequence typing (MLST) analysis indicated that 61 different sequence types (STs) were found (including 45 novel STs), and MLST did not show significantly dominant STs. Pulsed field gel electrophoresis (PFGE) results showed that 93 isolates could be divided into 73 clusters, and they also showed weak genetic linkages between isolates. The resistant rates to trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin were 9.7% and 4.3%, respectively, and all isolates were susceptible to minocycline. Four virulence gene’s loci Stmpr1, Stmpr2, Smf-1, and Smlt3773 were positive in 79.6%, 91.4%, 94.6%, and 52.7% of the isolates, respectively. Three biofilm genes rmlA, spgM, and rpfF were positive in 82.8%, 92.5%, and 64.5% of the isolates, respectively. Mean biofilm forming level of OD492 was 0.54 ± 0.49. We did not find any significant difference between different genders and different age-groups. We retrospectively analyzed data from patients in the intensive care unit (ICU) and the control group. The independent risk factors of those who were infected in the ICU included immunosuppression and the increased antibiotic usage.Conclusions: Most of the patients had prior medical usage histories and baseline diseases. The positive rate of virulence genes was high, the drug resistance rate of S. maltophilia was low, and the biofilm formation ability was strong. The increased use of antibiotics was an independent risk factor for S. maltophilia infection, which should receive more attention. No obvious clonal transmissions were found in the same departments.

show abstract

“…These ndings may be used as a new reference index for clinical sensitivity and control of S. maltophilia. Discussion S. maltophilia is an environmental global emerging gram-negative multiple-drug-resistant organism that is most commonly associated with respiratory infections in human beings [2,10]. It is also responsible for many other infectious diseases including bacteremia, endocarditis, and urethral infection, especially among the immunocompromised, as well as those undergoing aggressive treatments [1].…”

Section: Bio Lm Forming Abilitymentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Duan

Qin

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Stenotrophomonas maltophilia (S. maltophilia) is an important opportunistic pathogen that can be isolated in hospitals. With the abuse of broad spectrum antibiotics and invasive surgical devices, the rate of S. maltophilia infection is increasing every year. This study is an epidemiological analysis of the clinical and molecular characteristics of S. maltophilia infection in a Chinese teaching hospital. The goal is to obtain a comprehensive understanding of the status of S. maltophilia infection in order to provide strong epidemiological data for the prevention and treatment of S. maltophilia infection.Results: A total of 93 isolates from Renji Hospital affiliated to Shanghai Jiaotong University School of Medicine were included, in which 62 isolates were from male patients and 81 isolates were isolated from sputum samples. A total of 86 patients had underlying diseases. All of the patients had received antibiotic therapeutics. The multilocus sequence typing (MLST) analysis indicated that 61 different sequence types (STs) were found (including 45 novel STs), and the MLST did not show significantly dominant STs. Pulsed field gel electrophoresis (PFGE) results showed the 93 isolates could be divided into 73 clusters, and they also showed weak genetic linkage between isolates. The resistant rates to Trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin were 9.7% and 4.3%, respectively, and all of the isolates were susceptible to minocycline. The positive rates of the four virulence genes Stmpr1, Stmpr2, Smf-1, and Smlt3773 locus were 79.6%, 91.4%, 94.6%, and 52.7%, respectively. The positive rates of the three biofilm genes rmlA, spgM, and rpfF were 82.8%, 92.5%, and 64.5%, respectively. The mean biofilm forming level of OD492 was 0.54 ± 0.49, and there was no significant difference between different genders and different age groups. Data from patients in the intensive care unit (ICU) and the control group were analyzed retrospectively, and the independent risk factors of those who were infected in the ICU included immunosuppression and the increased of the antibiotics usage.Conclusions: Most of the patients had prior medical usage histories and baseline diseases. The positive rate of virulence genes was high, the drug resistance rate of S. maltophilia was low, and the biofilm formation ability was strong. The increased use of antibiotics was an independent risk factor for S. maltophilia infection, which should receive more attention. No obvious clonal transmission was found in the same department.

show abstract

A 10-year single-center experience on Stenotrophomonas maltophilia resistotyping in Szeged, Hungary

Cited by 19 publications

References 44 publications

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Contact Info

Product

Resources

About