A 1-Mb PAC Contig Spanning the Common Eliminated Region 1 (CER1) in Microcell Hybrid-Derived SCID Tumors

The transcriptional map of the common eliminated region 1 (C3CER1) in 3p21.3

“…15,16 This implies that C3CER1 contains one or several tumour growth antagonising genes. Our work fully defines the gene content of C3CER1, which is a prerequisite for understanding of its role in tumorigenesis.…”

“…16 However, in the course of the RP4-787C23 sequencing project, we realised that this clone was not overlapping with the sequence of RP6-123I13. This false overlap was initially based on the PCR analysis of a single STS marker (123i13-S.endB).…”

“…The cell lines MCH 906.8 and their SCID derived tumours were described previously. 16 DNA was prepared by proteinase K digestion and phenol/chloroform extraction according to standard protocol (Sambrook et al, 1989). The PCR-markers used for the genomic analysis were primers 22-23, 30-31, 32-33, 34-35, 41-42 and primer 49-50.…”

“…We therefore tested the MHC906.8 microcell hybrid-derived panel of SCID tumours that was used in our previous study. 16 We have used six new STS-es that are located in RP6-91P17 (primer 22-23 within TMEM7, primer 30-31, 32-33, 34-35 within LRRC2, primer 41-42 within LUZP3 and primer 49-50 within TDGF1). The last eliminated marker on the centromeric side of C3CER1 is primer 30-31, located in the 3'UTR region of LRRC2 and the first retained marker is primer 41-42 located in the LUZP3 gene, in the first intron of LRRC2 (Figure 4).…”

“…13 The putative tumour suppressor region (named common eliminated region 1, C3CER1) was initially restricted to *7 cM 14 and later to *1.6 cM 15 in 3p21.3 region. C3CER1 was covered by a PAC contig and its physical size was determined as approximately 1 Mb 16 . Construction of a detailed transcription map of C3CER1 is a requirement for the identification of a gene(s) with tumour inhibitory properties.…”