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Konno, Akira; Hoshino, Yutaka; Terashima, Shinya; Motoki, Ryoichi; Kawaguchi, Takanori

doi:10.1023/a:1013879702702

Cited by 69 publications

(31 citation statements)

References 34 publications

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“…Expression of aberrant forms of O‐glycans such as Tn antigen has been frequently observed in patients with CRC7, 43 and reported to be associated with tumour metastatic potential and poor prognosis 44, 45. However, whether defective O‐glycosylation occurs as a result of tumorigenesis or plays a contributing role in tumour development is unclear.…”

Section: Discussionmentioning

confidence: 99%

Aberrant O‐glycosylation contributes to tumorigenesis in human colorectal cancer

Jiang

Liu

et al. 2018

J Cellular Molecular Medi

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Aberrant O‐glycosylation is frequently observed in colorectal cancer (CRC) patients, but it is unclear if it contributes intrinsically to tumorigenesis. Here, we investigated the biological consequences of aberrant O‐glycosylation in CRC. We first detected the expression profile of Tn antigen in a serial of human CRC tissues and then explored the genetic and biosynthetic mechanisms. Moreover, we used a human CRC cell line (LS174T), which express Tn antigen, to assess whether aberrant O‐glycosylation can directly promote oncogenic properties. It showed that Tn antigen was detected in around 86% human primary and metastatic CRC tissues. Bio‐functional investigations showed that T‐synthase and Cosmc were both impaired in cancer tissues. A further analysis detected an occurrence of hypermethylation of Cosmc gene, which possibly caused its loss‐of‐function and a consequent inactive T‐synthase. Transfection of LS174T cells with WT Cosmc restored mature O‐glycosylation, which subsequently down‐regulated cancer cell proliferation, migration and apoptotic‐resistant ability. Significantly, the expression of MUC2, a heavily O‐glycosylated glycoprotein that plays an essential role in intestinal function, was uniformly reduced in human CRC tissues as well as in LS174T cells. These data suggest that aberrant O‐glycosylation contributes to the development of CRC through direct induction of oncogenic properties in cancer cells.

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Section: Discussionmentioning

confidence: 99%

Aberrant O‐glycosylation contributes to tumorigenesis in human colorectal cancer

Jiang

Liu

et al. 2018

J Cellular Molecular Medi

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“…Tumor-associated alterations of cell surface glycosylation play a crucial role for adhesion and metastasis of carcinoma cells [1, 2]. There is ample evidence that many, if not most of these alterations are represented by the production of extensively sialylated sugar chains in the oligosaccharide fractions of cell surface glycoproteins [3, 4].…”

Section: Introductionmentioning

confidence: 99%

Correlation Analysis between Tumorous Associated Antigen Sialyl-Tn Expression and ST6GalNAc I Activity in Human Colon Adenocarcinoma

Vázquez-Martín

Cuevas²,

Gil‐Martín

et al. 2004

Oncology

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Objectives: Sialyl-Tn (sTn) is a mucin carbohydrate-associated antigen that is strongly expressed in a large number of colorectal carcinomas. In this study, we combined immunohistochemical and enzymatic techniques in order to find the correlation between sTn tissue expression and the sialyltransferase activity (ST6GalNAc I) responsible for its synthesis in colorectal cancer (CRC) patients. Methods: We compared sTn expression in healthy (n = 46), tumorous (n = 60) and transitional tissue (n = 46) from CRC patients, and correlated sTn altered expression with clinicopathologic variables of the patient. Furthermore, we determined ST6GalNAc I tissue activity employing asialo-ovine submaxillary mucin (asialo-OSM) as glycoprotein acceptor (n = 27). Results: The rates of sTn positive expression obtained for healthy, tumorous and transitional tissues were 15, 67 and 63%, respectively. These rates led to statistically significant differences between healthy and tumorous or transitional tissue (p = 0.001); sTn expression was related to the first stages of the tumor invasion in transitional tissue. As regards ST6GalNAc I activity, we found an enhancement in transitional tissue. Statistical correlation analysis did not reveal association between sTn expression and ST6GalNAc I activity. Conclusions: Our findings indicated that sTn antigen tissue expression and ST6GalNAc I activity levels were not correlated in CRC, in spite of the overexpression of the antigen in tumorous and transitional tissue.

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Section: Introductionmentioning

confidence: 99%

“…It is hypothesized that the progression of cancer to an advanced stage is due to highly malignant and metastatic tumor cells arising within the primary tumor [3,4,5]. Recognition of such metastatic tumor cells should be useful for detecting the metastatic potential of CRC cell subpopulations [4, 5], which has been attributed to various cell properties, including sialylated complex carbohydrates [4,5,6,7,8]. CRC-related sialo-oligosaccharide structural changes are well documented [3,4,5,6,7,8,9], although there is still little information available concerning the regulated expression of sialyltransferase activities in human colon cancer tissues implied in their synthesis [10].…”

Section: Introductionmentioning

confidence: 99%

“…Recognition of such metastatic tumor cells should be useful for detecting the metastatic potential of CRC cell subpopulations [4, 5], which has been attributed to various cell properties, including sialylated complex carbohydrates [4,5,6,7,8]. CRC-related sialo-oligosaccharide structural changes are well documented [3,4,5,6,7,8,9], although there is still little information available concerning the regulated expression of sialyltransferase activities in human colon cancer tissues implied in their synthesis [10]. These studies are greatly hindered because sialyltransferase is weakly expressed and may have identical transfer activity towards the same acceptor [10, 11].…”

Section: Introductionmentioning

confidence: 99%

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Elevation of ST6Gal I Activity in Malignant and Transitional Tissue in Human Colorectal Cancer

Vázquez-Martín

Gil‐Martín²,

Fernández-Briera³

2005

Oncology

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Objectives: The aim of the present study was to investigate the activity of CMP-NeuAc:Galβ(1,4)GlcNAc sialyltransferase (ST6Gal I) in colorectal cancer (CRC). Methods: ST6Gal I activity was determined in healthy, transitional and tumor tissues from the same patient using asialotransferrin and N-acetyllactosamine as acceptors. Results: ST6Gal I activities with asialotransferrin (n = 85) and N-acetyllactosamine (n = 40) as acceptors were statistically significantly enhanced in CRC tissue compared with healthy mucosa from the same patient (p = 0.001). Using transitional tissue (n = 27), enhancement versus healthy tissue was observed (p < 0.05). A positive correlation was found between ST6Gal I activity with N-acetyllactosamine and asialotransferrin in healthy (n = 32), tumorous (n = 32) and transitional tissue (n = 27), supporting the fact that the same enzyme was detected using both acceptors. Furthermore, we studied the relationship between some patients’ clinicopathological features and ST6Gal I activity. Although the differences were not statistically significant, the levels of ST6Gal I activity in tumorous and transitional tissues varied with the histological grade of the tumor; however, we failed to find a correlation with the AJCC tumor classification. Conclusions: This work reports enhanced ST6Gal I activity in tumor and transitional tissues from CRC patients. However, our overall results suggest that ST6Gal I activity is not indicative of the patient’s outcome.

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Cited by 69 publications

References 34 publications

Aberrant O‐glycosylation contributes to tumorigenesis in human colorectal cancer

Aberrant O‐glycosylation contributes to tumorigenesis in human colorectal cancer

Correlation Analysis between Tumorous Associated Antigen Sialyl-Tn Expression and ST6GalNAc I Activity in Human Colon Adenocarcinoma

Elevation of ST6Gal I Activity in Malignant and Transitional Tissue in Human Colorectal Cancer

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