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Abstract

Nanomaterial stimulated alteration in the neuron is a great concern as the nanoparticles are widely used in the diagnosis of some neural disorders. Among the different nanoparticles, gold nanoparticles have a wide scope of applicability in the living system due to less toxicity. Gold nanoparticles can be used for cancer therapy especially for the killing of malignant cells in the brain. These particles can cross the bloodbrain barrier and can change the physiological functions of the brain. Gold nanoparticles may cause the change of different membrane proteins that might be responsible for defective transport of chemical through the membrane and finally leads to cellular loss. The experiment has been designed to study the changes of membrane polarity and intracellular calcium level in gold nanoparticles exposed cultured neuron. Citratestabilized gold nanoparticles are prepared from chloro-auric acid using sodium citrate as a stabilizing agent.Gold nanoparticles are characterized by UV Vis-spectrophotometer. Intracellular calcium level is monitored using fluorescence dye fluo 3. Membrane depolarization is studied using voltage-sensitive dye DiBAC(4)3. A higher level of intracellular calcium is observed in AuNP treated neurons. Elevation of intracellular calcium level might be due to the release of calcium from the intracellular storage or transport from extracellular media.Membrane depolarization and hyperpolarization study reveals that AuNP treated neurons membrane is present in depolarized state in comparison with the control neurons. A higher level of intracellular calcium and prolong depolarization in AuNP treated neuron may trigger some signaling mechanism leading to neuronal death