The three-dimensional organization of telomeres in the nucleus of mammalian cells

Chuang, Tony C. Y.; Moshir, Sharareh; Garini, Yuval; Chuang, Alice; Young, Ian T.; Vermolen, Bart J.; Doel, Richard van den; Mougey, Virginie; Perrin, Mathilde; Braun, Martina; Kerr, Paul; Fest, Thierry; Boukamp, Petra; Mai, Sabine

doi:10.1186/1741-7007-2-12

Cited by 132 publications

(72 citation statements)

References 31 publications

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“…Telomere clustering was detected in both undifferentiated and terminally differentiated cells. It seems that this is a characteristic feature of interphase nuclei arrangements, since it was observed in many cell types, including lymphocytes, neutrophils, and cancer cells (Chuang et al 2004;Weierich et al 2003;Yerle-Bouissou et al 2009). Since mammalian HP1α localizes with pericentromeric heterochromatin in the interphase, we also analysed the distribution of chromocenters.…”

Section: Discussionmentioning

confidence: 99%

Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

Stachecka

Walczak

Kociucka

et al. 2017

Histochem Cell Biol

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for further study, a comparative characteristic of the nuclear organization in BM-MSCs and AD-MSCs was performed. Nuclear substructures were visualized by indirect immunofluorescence (nucleoli, nuclear speckles, PML bodies, lamins, and HP1α) or fluorescence in situ hybridization (telomeres) on fixed cells at 0, 3, 5, and 7 days of differentiation. Comprehensive characterization of these structures, in terms of their number, size, dynamics, and arrangement in three-dimensional space of the nucleus, was performed. It was found that during differentiation of porcine MSCs into adipocytes, changes of nuclear organization occurred and concerned: (1) the nuclear size and shape; (2) reduced lamin A/C expression; and (3) reorganization of chromocenters. Other elements of nuclear architecture such as nucleoli, SC-35 nuclear speckles, and telomeres showed no significant changes when compared to undifferentiated and mature fat cells. In addition, the presence of a low number of PML bodies was characteristic of the studied porcine mesenchymal stem cell adipogenesis system. It has been shown that the arrangement of selected components of nuclear architecture was very similar in MSCs derived from different sources, whereas adipocyte differentiation involves nuclear reorganization. This study adds new data on nuclear organization during adipogenesis using the pig as a model organism.

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Section: Discussionmentioning

confidence: 99%

Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

Stachecka

Walczak

Kociucka

et al. 2017

Histochem Cell Biol

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“…Telomere measurements were done with TELOVIEW (9,46). By choosing a simple threshold for the telomeres, a binary image is found.…”

Section: D Image Analysis For Telomeresmentioning

confidence: 99%

“…We recently reported that the normal interphase nucleus has a unique 3D telomeric organization that is cell cycle dependent (9,10). Telomeres are organized in a nonoverlapping manner and align into a central telomeric disk during the late G 2 phase of the cell cycle (9).…”

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confidence: 99%

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c-Myc induces chromosomal rearrangements through telomere and chromosome remodeling in the interphase nucleus

Louis

Vermolen

Garini

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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143

204

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In previous work, we showed that telomeres of normal cells are organized within the 3D space of the interphase nucleus in a nonoverlapping and cell cycle-dependent manner. This order is distorted in tumor cell nuclei where telomeres are found in close association forming aggregates of various numbers and sizes. Here we show that c-Myc overexpression induces telomeric aggregations in the interphase nucleus. Directly proportional to the duration of c-Myc deregulation, we observe three or five cycles of telomeric aggregate formation in interphase nuclei. These cycles reflect the onset and propagation of breakage-bridge-fusion cycles that are initiated by end-to-end telomeric fusions of chromosomes. Subsequent to initial chromosomal breakages, new fusions follow and the breakage-bridge-fusion cycles continue. During this time, nonreciprocal translocations are generated. c-Myc-dependent remodeling of the organization of telomeres thus precedes the onset of genomic instability and subsequently leads to chromosomal rearrangements. Our findings reveal that c-Myc possesses the ability to structurally modify chromosomes through telomeric fusions, thereby reorganizing the genetic information.genomic instability ͉ 3D nucleus ͉ breakage-bridge-fusion

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“…The recent trend to record and analyze the early genomic events associated with transition through telomere crisis and aggregation is, detection of telomeric FISH signals using 3D epifluorescent or confocal laser scanning microscopes with adjusted fluorescence standard. Telomeric aggregates are suggestive of genomic instability and it is possible to examine the aggregates in individual interphase nuclei and tissues without the need to examine metaphases [76]. The next step is to analyze the 3D images visually and also by using specially designed software for quantitation [40,76].…”

Section: Future Directions In the Study Of Telo-meres In Breast And Omentioning

confidence: 99%

Telomeres, Genomic Instability, DNA Repair and Breast Cancer

Mai¹

2005

CMCAIAA

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This review describes our current understanding of telomere length polymorphism and organization in breast cancer cells. The key roles of the telomeric DNA protein complexes as protectors of chromosomal ends in normal and cancer cells are under much attention in current research, and here we review some of these issues. In general the functionality of telomeres depends on a number of associated factors, like -(1) the length of the telomeric sequence at a particular chromosomal end, (2) the status of telomerase activity, (3) the telomere associated proteins, (4) the status of subtelomeric heterochromatinization, (5) or associated chromosomal instability. Specifically, in breast cancer, depending on the presence or absence of the mutations in DNA repair proteins the telomere function becomes much more fragile. Some recent studies using modern high performance three-dimensional (3D) imaging technologies indicate that many exciting aspects of this multifaceted telomere research are going to unfold further in the coming years.

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The three-dimensional organization of telomeres in the nucleus of mammalian cells

Cited by 132 publications

References 31 publications

Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

c-Myc induces chromosomal rearrangements through telomere and chromosome remodeling in the interphase nucleus

Telomeres, Genomic Instability, DNA Repair and Breast Cancer

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