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Abstract

19F NMR spectroscopy was used to determine quantitatively the organ distribution and organ retention time in rats of the mixed fluorocarbon-hydrocarbon dowel molecule C6F13CH = CHC10H21 (F6H10E), which stabilizes highly concentrated injectable fluorocarbon emulsions destined for in vivo oxygen transport and delivery. The only fluorine resonances detected in the 19F NMR spectra of the organs analyzed were those of the F6H10E dowel itself, indicating that metabolites, if present, have very low concentrations (< 10(-4) M, limit of our assay). The F6H10E content in the liver peaked 1 day after administration (7 days for the spleen). At a dose of 3.6 g/kg body weight, the half-life of F6H10E in the liver was 25 +/- 5 days.

Regioselective Porphyrin Bridge Cleavage Controlled by Electronic Effects. Coupled Oxidation of 3-Demethyl-3-(trifluoromethyl)mesohemin IX and Identification of Its Four Biliverdin Derivatives

“…Data are recorded as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet), relative intensities, and identification. For 19 F NMR, a 60° pulse angle and a 2.2-s relaxation delay, which was longer than 10 T 1 , were used . Two-dimensional phase-sensitive ROESY spectra were obtained with a mixing time of 200 ms. UV−vis spectra were recorded on a Hewlett-Packard 8452A diode array spectrophotometer with a thermostated cell compartment.…”

Fluorocarbons and Fluorosurfactants for In Vivo Oxygen Transport (Blood Substitutes), Imaging, and Drug Delivery

“…23 They also form micelles and gels in both fluorocarbons and hydrocar bons, 24,25 as well as bilayer-type crystals in the solid State; 26 they were shown to provide outstanding stabilization of fluorocarbon-in-water emulsions and control over their particle size; 27,28 when added to the fluorocarbon phase, they augment the solubility of lipophilic material; when incorporated in the bilayer membrane of liposomes, they strongly increase the stability of these liposomes, reduce their permeability, and slow down their fusion; 2,29,30 they can even hinder the enzymatic hydrolysis of the bilayer's phospholipids." 35 Colloids Based on the Self-Assembly of Fluorinated Amphiphiles Fluorinated chains confer to amphi philes a strong propensity to self-assemble into bilayer-and nonbilayer-based nanoand microstructures, including vesicles, tubules, helices, ribbons, and other supramolecular assemblies 1 ' 2 ' 13 ' 19,36 " 39 (Figure 3). 32 Their synthesis is straightforward and cost-effective.…”

Method Performance and Validation for Quantitative Analysis by 1H and 31P NMR Spectroscopy. Applications to Analytical Standards and Agricultural Chemicals

“…Recently, a method for quantitative determination of compounds in continuous-flow LC NMR has been reported . Although 1 H, 13 C, and 31 P QNMR methods are the most frequently applied, QNMR methods employing other nuclei have also been reported. − …”

“…47 Although 1 H, 13 C, and 31 P QNMR methods are the most frequently applied, QNMR methods employing other nuclei have also been reported. [48][49][50][51][52][53][54][55] Many of the earlier QNMR measurements showed relative standard deviations as high as 5%, which is unsatisfactory for most analytical purposes, and many did not include an error analysis.…”