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Abstract

ABSTRACT:One of the many challenges of bioinformatics, now a day; is the integration of biological data.The establishment of large quantities of data concerning the differentially expressed genes during infection with an intracellular pathogen requires the implementation of integration strategies to gather all the data and therefore a better understanding of the functioning of the Intracellular infection. This work aims to exploit the bioinformatics approach, to provide a new interpretation of the data available in the literature as well as databases on the effect of intracellular infection, cases of the Leishmania parasite, on the host. A total of 30 genes expressed differently from a Leishmania parasite infection were used for functional analysis using the bioinformatics tool. The latter provides rapid assessment of signaling and metabolic pathways, molecular networks and biological processes that are more significantly disrupted in a data set of interest. Bioinformatics analysis revealed that apoptosis, cytokine production, and signaling were altered following infection with the Leishmania parasite. The genes of IL-10, IL-4, IL-6, SOD1, EIF2AK2, NF-kB and PI3K/akt were most activated after Infection by the Leishmania parasite. The results suggest that the Leishmania parasite reverses immune and inflammatory responses, meaning the ability to induce programmed cell death and microbicidal functions of macrophages such as inhibition of nitric oxide (NO), And the functions of the inducible cytokines of macrophages. Analyzing and interpreting data using the bioinformatics approach helps to unlock knowledge buried in experimental data by quickly identifying links, mechanisms, functions and main pathways to move beyond Statistical analysis to new biological analyzes.