Untitled

Etienne, Hervé; Berthouly, Marc

doi:10.1023/a:1015668610465

Cited by 320 publications

(123 citation statements)

References 44 publications

Supporting

Mentioning

119

Contrasting

Unclassified

Order By: Relevance

“…Ducos et al (1993) reported the production of 600,000 embryos L −1 of medium in a bioreactor. The use of the method known as automated temporary immersion system (recipient à immersion temporaire automatique (RITA)) has been shown to be beneficial for the production and germination of somatic embryos of coffee (Etienne and Berthouly 2002;Etienne-Barry et al 1999). This method yielded synchronous production and germination of somatic embryos of C. arabica F1 hybrids Etienne et al 1997a) without the need of selection before acclimatization another advantage of the uses of RITA (Etienne-Barry et al 1999).…”

Section: Scale-upmentioning

confidence: 99%

Somatic Embryogenesis in Coffea spp.

Loyola‐Vargas

Avilez-Montalvo

Aviles-Montalvo

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

View full text Add to dashboard Cite

Coffee is a very important commodity in the world. It is a perennial plant and as a consequence its genetic improvement had been difficult. The biotechnology, in particular somatic embryogenesis (SE) has helped to the propagation of commercial coffee cultivars. Along almost 50 years, different research groups around the world have developed uncountable SE processes to produce an efficient, reproducible, and reliable SE process. Many of the factors that affect the embryogenic response have been studied. It has been demonstrated that the genetic modification of coffee is possible. This transformation can lead to the improvement of varieties with very attractive agronomic traits, such as low caffeine content, resistant to diseases and pests. IntroductionCoffee is one of the two major commodities in the world. The cultivation of coffee gives employment to more than 80 million people, and every year, more than 11 million hectares are cultivated around the world.All coffee species are trees or perennial woody bushes. The coffee plants differ among themselves in different aspects such as morphology, size, and ecological adaptation. Although there are more than 124 species of the Coffea genus, only two are used to produce coffee commercially, Coffea arabica and C. canephora, which accounts for 60 and 40 % of world coffee production, respectively. C. canephora (2n = 2x = 22) is an outcrossing diploid, allogamous, consisting of polymorphic populations of strongly heterozygous individuals and with high caffeine content. C. arabica is an allotetraploid (2n = 4x = 44) derived from a spontaneous

show abstract

Section: Scale-upmentioning

confidence: 99%

Somatic Embryogenesis in Coffea spp.

Loyola‐Vargas

Avilez-Montalvo

Aviles-Montalvo

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

View full text Add to dashboard Cite

show abstract

“…The two-vessel TIBs (one holds the liquid medium and the other the cultures of the plants) proved to be a more flexible approach with relatively simple construction and operational ease. Furthermore, TIBs might be optimized using a spectrum of glass/plastic containers and thus appropriate for largescale plant micropropagation facilities [30]. Previously Debnath [18] reported about lowbush blueberry micropropagation using RITA  bioreactors (VITROPIC, SaintMathieu-de-Treíviers, France; [31]), which could be considered as a more expensive alternative.…”

Section: Discussionmentioning

confidence: 99%

An Approach for Micropropagation of Blueberry (<i>Vaccinium corymbosum</i> L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)

Arencibia¹,

Vergara²,

Quiroz³

et al. 2013

AJPS

View full text Add to dashboard Cite

A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO 2 enrichment, sucrose-reduced medium, and light intensity of 60 M m −2 ·s −1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.

show abstract

“…This system was therefore adopted for selecting putatively transformed embryos, as an alternative to the conventional method of selection on semi-solid medium. Although the use of TIS for mass clonal propagation has increased (Etienne and Berthouly 2002;Watt 2012), reports of its application for the selection phase of genetic transformation studies are scarce and involve genetic transformation based on adventitious shoot regeneration in pineapple (Espinosa et al 2002) and strawberry (Hanhineva and Kärenlampi 2007), or adventitious root formation in sage (Marchev et al 2011). However, none of these studies compared the transformation efficiency of TIS and semi-solid medium.…”

Section: Discussionmentioning

confidence: 99%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

As the limiting factor for recovery of plants from oak embryogenic lines is the low embryo conversion rate, axillary shoot lines were established from transformed germinated embryos.Transformed embryos and shoots were cultured in medium with or without kanamycin and the responses to several morphogenetic processes (recovery after cryopreservation, germination, shoot proliferation, and rooting) were evaluated.2

show abstract

Untitled

Cited by 320 publications

References 44 publications

Somatic Embryogenesis in Coffea spp.

Somatic Embryogenesis in Coffea spp.

An Approach for Micropropagation of Blueberry (<i>Vaccinium corymbosum</i> L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Contact Info

Product

Resources

About

Untitled

Cited by 320 publications

References 44 publications

Somatic Embryogenesis in Coffea spp.

Somatic Embryogenesis in Coffea spp.

An Approach for Micropropagation of Blueberry (&lt;i&gt;Vaccinium corymbosum&lt;/i&gt; L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Contact Info

Product

Resources

About

An Approach for Micropropagation of Blueberry (<i>Vaccinium corymbosum</i> L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)