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Hansson, Therése; Adlercreutz, Patrick

doi:10.1023/a:1019875116426

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…16,17 However, most b-glycosidases have both b-galactosidase and b-glucosidase activities, even if they are referred on the basis of their dominant activity, as either b-glucosidases or b-galactosidase. 18 Before biotransformation, the Rg3 inclusion complex was prepared in order to improve water solubility, whereas Rb1 needed no treatment.…”

Section: Introductionmentioning

confidence: 99%

Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using β-galactosidase from Aspergillus sp.

Wan

2015

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using β-galactosidase from Aspergillus sp.

Wan

2015

RSC Adv.

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Developing Promiscuous Glycosidases for Glycoside Synthesis: Residues W433 and E432 in Sulfolobus solfataricus β‐Glycosidase are Important Glucoside‐ and Galactoside‐Specificity Determinants

Hancock¹,

Corbett²,

Fordham-Skelton³

et al. 2005

ChemBioChem

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Two residues that have been implicated in determining the substrate specificity of the thermophilic beta-glycosidase from the archaeon Sulfolobus solfataricus (SsbetaG), a member of the glycosyl hydrolase family 1, have been mutated by site-directed mutagenesis so as to create more versatile catalysts for carbohydrate chemistry. The wild-type and mutated sequences were expressed in E. coli with a His(7)-tag to allow one-step chromatographic purification. The E432C and W433C mutations removed key interactions with the OH-4 and OH-3 of the sugar substrates, thus reducing the discrimination of glucose, galactose and fucose with respect to other glycosides. This resulted in two glycosidases with greatly broadened substrate specificities. Observed changes include a 24-fold increase in Man:Gal activity and an 18-fold increase in GalA:Gal activity. This promiscuous substrate tolerance was further illustrated by the parallel synthesis of a beta-glycoside library of glucose, galactose, xylose and mannose in one pot at 50 degrees C, in organic solvent. The synthetic potential of the catalysts was further evaluated through alkyl glycoside transglycosylation yields, including the first examples of synthesis of beta-mannosides and beta-xylosides with SsbetaG.

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Production of steviol from steviol glucosides using β-glycosidase from Sulfolobus solfataricus

Nguyen

Kim

et al. 2016

Enzyme and Microbial Technology

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Cited by 6 publications

References 18 publications

Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using β-galactosidase from Aspergillus sp.

Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using β-galactosidase from Aspergillus sp.

Developing Promiscuous Glycosidases for Glycoside Synthesis: Residues W433 and E432 in Sulfolobus solfataricus β‐Glycosidase are Important Glucoside‐ and Galactoside‐Specificity Determinants

Production of steviol from steviol glucosides using β-glycosidase from Sulfolobus solfataricus

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