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Salma, Farah Dina; Rahayu, Ira Gustira; Rinaldi, Sonny Feisal; Kurnaeni, Nani

doi:10.34011/juriskesbdg.v11i1.797

Cited by 4 publications

(1 citation statement)

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“…or the quality of clinical examination results (6). Control materials can be obtained from commercial or prepared from pond materials (secondary control materials).…”

Section: Control Materials Used In Laboratories To Monitor the Accura...mentioning

confidence: 99%

Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks

Hartani

Handayati

2023

IJMLST

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Improving health laboratory services is closely related to strengthening the quality of health laboratories. Serum control is part of the quality assurance of health laboratories. Lyophilized homemade serum is a type of homemade control serum. This research aims to determine the stability of lyophilized homemade control serum against Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels. This research used an experimental method with pooled sera as research material stored at -2°C to -4°C and -20°C for 8 weeks. Pooled sera were obtained from respondents who had no history of disease, were free from HIV, AIDS, and HBsAg. The research was conducted at the Reference Laboratory and Clinical Chemistry Laboratory Politeknik Kesehatan (Poltekkes) Kementerian Kesehatan, Surabaya. The results of the study were data analysis using linear regression test. The SGOT parameter at freezer temperature (-2 to -4°C) showed that the storage time had an effect of 90.97% with an error component of 9.03%. At freezer temperature (-20°C), the result of storage time has an effect of 78.71% with an error component of 21.29%. In the SGPT parameter with freezer temperature (-2 to -4°C), the result of storage time has an effect of 96.71% with an error component of 3.29%. At freezer temperature (-20°C), the result of storage time has an effect of 91.47% with an error component of 8.53%. The results of the SGOT and SGPT examinations did not exceed the limits of ±2SD and ±3SD The CV on the SGOT and SGPT examinations also did not exceed the CCV limits, which is to be stable.

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“…or the quality of clinical examination results (6). Control materials can be obtained from commercial or prepared from pond materials (secondary control materials).…”

Section: Control Materials Used In Laboratories To Monitor the Accura...mentioning

confidence: 99%

Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks

Hartani

Handayati

2023

IJMLST

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The Effect of Storage Time for Pooled Sera on Freezers on the Quality of Clinical Chemical Examination

Maulidiyanti

Purwaningsih²,

Widiyastuti³

et al. 2021

medicra

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A common problem in clinical laboratories is maintaining the stability of serum analytes during sample storage. Samples are usually stored in the door (4–8°C) of the refrigerator for a short period of time or in the freezer (-20°C) for a longer period of time (pre-analytical stage) and subsequently, during post-analysis sample handling (storage time and temperature). This study aims to analyze the stability in this case is the storage time of pooled sera in the freezer at temperatures up to -18º C and in the refrigerator at temperatures up to 4°C for 3 months with the inspection parameters, namely cholesterol and triglycerides. This type of research is experimental, with a research design that is time series control group design. The sample in this study was a control serum in the form of pooled sera stored in the freezer and refrigerator which was checked for cholesterol and triglyceride levels every 2 weeks for 3 months or for 12 weeks. The results showed that the average calculation of cholesterol levels in pooled sera stored in the freezer was 142.07 mg/dL and the average cholesterol level examination stored in the refrigerator was 161.89 mg/dL then the average the examination of triglyceride levels in pooled sera stored in the freezer is 112.89 mg/dL and the average examination of triglyceride levels stored in the refrigerator is 140.46 mg/dL. Based on the analysis of the research results, it can be concluded that, there is an effect of storage time in the freezer on the stability of cholesterol levels and triglyceride levels.

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Bovine Serum as an Alternative to Control Serum for Total Protein Levels

Supriyanta

Martsiningsih

Soenjono

et al. 2021

Open Access Maced J Med Sci

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Total protein describes the liver's ability to synthesize proteins and metabolize substances in the blood. Bovine serum can be used as a control material because the total protein analyte in bovine blood serum is almost identical to the analyte in human serum. The researchers intend to determine the homogeneity and stability of bovine serum with the addition of 7,5% ethylene glycol preservative after being stored for 12 weeks at -20°C as an alternative to control serum for total protein levels. This research used survey analytics using a one-group pretest-posttest research design. Data analysis used ISO 13528 2005 calculations. From the examination of the total protein level in the serum, the value of Xr – Yr = 0.23817, which is where the value meets the test criteria according to | Xr - Yr | <0.36, that is 0.23817 <0.91585. The total protein content of bovine serum was homogeneous and stable with the addition of 7.5% ethylene glycol stored at -20oC for 12 weeks.

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Cited by 4 publications

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Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks

Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks

The Effect of Storage Time for Pooled Sera on Freezers on the Quality of Clinical Chemical Examination

Bovine Serum as an Alternative to Control Serum for Total Protein Levels

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