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Abstract

The antibacterial activity of Pulicaria inuloides and Pulicaria crispa essential oils was tested against Listeria monocytogenes, Escherichia coli and Staphylococcus aureus using agar disc diffusion and microtiter broth microdilution assays. PIEO, the essential oil from Pulicaria inuloides, inhibited all tested microorganisms with a minimum inhibitory concentration (MIC) of 5.7, 0.12 and 5.11 mg/ml against Listeria monocytogenes, Escherichia coli and Staphylococcus aureus respectively. PCEO, the essential oil from Pulicaria crispa, showed a lower inhibition for all of the tested microorganisms. The present study evaluated the impact of PIEO on L. monocytogenes, E. coli, S. aureus and tap water (control) inoculated in fish fillets. Results indicated that PIEO eliminated most of L. monocytogenes, E. coli, S. aureus and SPC immediately after treatment at time zero (25°C) and during storage at 10°C for 12 days, and it increased the shelf -life of seafood during storage at 10°C compared with the tap water, whereas SPC counts were high than 7 log CFU/g at the end of storage.The sensory evaluation of fillet fish treated with Pulicaria inuloides essential oil were acceptable by consumers at the level of 0.1 g/100g EO. Fish purchased from supermarkets in Wuxi province, Jiangsu, China, were maintained with good hygiene practices and health conditions, whereas fish purchased from local markets were not.

The Role of Essential Oils against Pathogenic Escherichia coli in Food Products

“…The study carried out by Abdeldaiem et al [52] explored the use of rosemary, cinnamon, fennel, and cardamom EOs to prevent the proliferation of E. coli in carp flesh fingers. The authors observed that the inhibitory effect was obtained for all tested EOs, particularly in the first six days of storage at 4 • C. In the same line, Al-Hajj et al [53] evaluated the impact of Pulicaria inuloides EO on E. coli inoculated in Kachlan (Trachinotus ovatus, Linnaeus) fillets and obtained reduction of 1 log CFU/g.…”

Effect of Moringa and Olive Leaves Powder Consumption on Hyperglycemic State in Type 2 Diabetes Mellitus (A Pilot Study)

“…The glucose-lowering benefits of oral antidiabetic drugs (OAD) have been well established. But all the drugs are not absolutely safe in a patient having CV disease [22,23]. The other two groups also showed slight statistical significance in the drug group more than the herbs, but when we followed the individual samples, the results showed a marked improvement in the herbs group more than the drug, and the reason for this is that the sample was very few compared to the sample of the drug, which was much larger.…”

Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation

“…Then two strains of E.coli and B. cereus approximately 6 log 10 CFU/g were inoculated using micropipettes on each side of fish fillet pieces to gain a final concentration of 4 log 10 CFU/g. Next, samples were dipped in solutions of ChNPs 1% and AgNPs 40.0µg/ml (w/g) water for 1 min to enable attachment (ratio of 1:3) (Al-Hajj et al, 2017), and cumin and marjoram essential oil were added to fish fillets samples appropriate volumes to the surface (two sides) of each fish fillet using micropipette so as to achieve at 500 ppm oil volume per fish flesh weight (v/w) respectively, Goulas & Kontominas (2007) (Table 1), After that, all fish fillets samples were packaged in a foam plates, wrapped with polyethylene and stored at 4±1C up to 12 days. The samples were taken for analysis every 3 days periodically.…”