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Kaul, Gautam; Amiji, Mansoor M.

doi:10.1023/a:1016486910719

Cited by 258 publications

(60 citation statements)

References 19 publications

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“…Properties of Nanoparticles: Gelatin and PEGylated gelatin nanoparticles of approximately 200 nm in diameter with spherical shape, as shown by scanning electron microscopy [5], were prepared for encapsulation and delivery of plasmid DNA to solid tumors. In addition, previous in vitro studies showed that both gelatin and PEGylated gelatin nanoparticles could encapsulate plasmid DNA (EGFP-NI, Clontech) at around 98% efficiency at 0.5% (wlw) loading levels.…”

Section: Resultsmentioning

confidence: 99%

“…Nanoparticle Preparation and Characterization: PEG-modified gelatin was synthesized by reacting Type-B gelatin with PEG-epoxide as previously described [5]. Nanoparticles of the unmodified gelatin and PEGylated gelatin derivative were prepared by the ethanol precipitation method under controlled conditions of temperature and pH.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…Any unreacted aldehyde groups of glyoxal were quenched with aqueous 12% (w/v) sodium metabisulfite and the nanoparticle precipitate was centrifuged at 14,000 rpm for 90 minutes, washed twice with deionized distilled water, and were lyophilized. The nanoparticles were characterized by Coulter particle size analysis as previously described [5].…”

Section: Experimental Methodsmentioning

confidence: 99%

“…In a review article Moghimi et al, [4] have extensively discussed the development and applications of long-circulating and target-specific nanoparticles. Previously, we have described the preparation and characterization of PEG-modified (PEGylated) gelatin nanoparticles as a hydrophilic, biocompatible, biodegradable matrix for delivery of hydrophilic molecules, such as plasmid DNA, to solid tumors [5]. Type B gelatin was modified with PEG-epoxide and nanoparticles were formed, under controlled temperature and pH, by solvent displacement using water-ethanol mixture.…”

Section: Introductionmentioning

confidence: 99%

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Biodistribution and Tumor-Targeting Potential of Poly(Ethylene Glycol)-Modified Gelatin Nanoparticles

Kaul

Amiji

2004

MRS Proc.

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In order to develop a safe and effective systemically-administered delivery system for solid tumors, the biodistribution of control gelatin and poly(ethylene-glycol) modified (PEGylated) gelatin nanoparticles was examined in Lewis lung carcinoma (LLC)-bearing female C57BL6 mice. Type B gelatin and PEGylated gelatin nanoparticles were radiolabeled ( 251) for the in vivo biodistribution studies after intravenous (i.v.) administration through the tail vein in LLC-bearing mice. At various time intervals, the tumor-bearing mice were sacrificed and tumor, blood, and major organs were harvested for analysis of radioactivity corresponding to the localization of the nanoparticles. Percent recovered dose was determined and normalized to the weight of the tissue or fluid sample. Non-compartmental pharmacokinetic analysis was performed to determine the long-circulating property and preferential tumor targeting potential of PEGylated gelatin nanoparticles in vivo. From the radioactivity in plasma and various organs collected, it was evident that the majority of PEGylated nanoparticles were present either in the blood pool or taken up by the tumor mass and liver. For instance, after 3 hours, the PEGylated gelatin nanoparticles were almost 2-fold higher in the blood pool than the control gelatin nanoparticles. PEGylated gelatin nanoparticles remained in the blood pool for a longer period of time due to the steric repulsion effect of the PEG chains as compared to the control gelatin nanoparticles. In addition, approximately 4-5% of the recovered dose of PEGylated gelatin nanoparticles was present in the tumor mass for up to 12 hours. The plasma and the tumor half-lives, area-underthe-curve, and the mean residence time of the PEGylated gelatin nanoparticles were significantly greater than those of the control gelatin nanoparticles. The results of the study confirmed longcirculating property and preferential tumor targeting potential of PEGylated gelatin nanoparticles in a murine tumor model.

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Section: Resultsmentioning

confidence: 99%

Section: Experimental Methodsmentioning

confidence: 99%

Section: Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biodistribution and Tumor-Targeting Potential of Poly(Ethylene Glycol)-Modified Gelatin Nanoparticles

Kaul

Amiji

2004

MRS Proc.

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“…This was further demonstrated by Kushibiki and Tabata in 2005 [146] using DNA-containing poly(ethylene glycol)-modified (PEGylated) gelatin nanoparticles. PEGylation of gelatin also proved beneficial to long-circulating delivery system in vivo and also for targeting tumor cells, such as BT/20 human breast cancer cells [147].…”

Section: Application Of Nanoparticles In Drug Deliverymentioning

confidence: 99%

Biopolymeric Nanoparticles

2017

Design of Nanostructures

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This review on nanoparticles highlights the various biopolymers (proteins and polysaccharides) which have recently revolutionized the world of biocompatible and degradable natural biological materials. The methods of their fabrication, including emulsification, desolvation, coacervation and electrospray drying are described. The characterization of different parameters for a given nanoparticle, such as particle size, surface charge, morphology, stability, structure, cellular uptake, cytotoxicity, drug loading and drug release, is outlined together with the relevant measurement techniques. Applications in the fields of medicine and biotechnology are discussed along with a promising future scope.

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Biodegradable Nanoparticles

Chakravarthi

Robinson

2010

Pharmaceutical Sciences Encyclopedia

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Biodegradable nanoparticles are a very active area of research in drug delivery, imaging, and diagnostics. The use of nanoparticles in drug delivery and nanomedicine necessitate use of biocompatible and biodegradable polymeric carriers for parenteral administration. This article focuses on the application of nanotechnology to deliver therapeutic or diagnostic agents using biodegradable polymeric nanoparticles for systemic, localized, or targeted delivery.

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Abstract: The results of this study show that PEGylation of gelatin may prove beneficial as long-circulating delivery system in vivo. Additionally, the nanoparticles could encapsulate hydrophilic macromolecules and are internalized by tumor cells.

Cited by 258 publications

References 19 publications

Biodistribution and Tumor-Targeting Potential of Poly(Ethylene Glycol)-Modified Gelatin Nanoparticles

Biodistribution and Tumor-Targeting Potential of Poly(Ethylene Glycol)-Modified Gelatin Nanoparticles

Biopolymeric Nanoparticles

Biodegradable Nanoparticles

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