Untitled

Abstract

Gram negative sepsis and septic shock continue to be a major medical problem, with a complex physiopathology and it is associated with high mortality. Although secretion of cytokines such as tumor necrosis factor-alpha by macrophages is the principal host mediator of septic shock, other characteristic functions of macrophages implicated in their phagocytic capacity have not been studied in the process of endotoxic shock. In the present study we have used an intraperitoneal injection of E. coli lipopolysaccharide (LPS) (100 mg/kg) in order to obtain an endotoxic shock model in adult female BALB/c mice. Peritoneal cell suspensions were obtained at several times (2, 4, 12 or 24 h) after injection and the following functions were studied on the peritoneal macrophages: adherence to substrate, mobility (spontaneous and directed or chemotaxis), ingestion of particles and superoxide anion production. The results showed a stimulation of adherence, ingestion and superoxide production as well as a decrease of chemotaxis in the animals injected with LPS. These effects changed with time after LPS injection. Thus, the increase of adherence and the decrease of mobility were higher during the first hours, whereas the increase in ingestion and superoxide production turned larger with time.

The Methanol Extract of Azadirachta indica A. Juss Leaf Protects Mice Against Lethal Endotoxemia and Sepsis

“…Pro-inflammatory molecules such as NO and TNF-α were examined to evaluate the anti-inflammatory effect of NLE because upon bacterial LPS stimulation, these molecules are known to induce oxidative stress, vascular anomalies such as disseminated intravascular coagulation, vascular perfusion, hypotension, multiple organ failure, and, in severe cases, septic shock and death (MacMicking et al ., 1995; Mayeux, 1997; Víctor et al ., 1998; Yazar et al ., 2010). In order to explore the in vivo effect of NLE, an LPS-induced mouse model of sepsis was established, using specific pathogen-free C57BL/6 mice.…”

Effects of low power laser radiation of blue, green and red ranges on free radical processes in rat blood in endotoxic shock

“…Полученные результаты согласуются с данными литературы [16] и свидетельствуют о снижении продукции лейкоцитами активных форм кислорода, продуцируемых NADPH-оксидазой и миелопероксидазой, а также об угнетении способности к хемотаксису и агрегации при эндотоксиновом шоке [16][17][18]. Такое угнетение функций лейкоцитов при введении ЛПС является следствием чрезмерной активации этих клеток, вследствие прямого контакта рецепторов лейкоцитов с ЛПС, что в конечном итоге приводит к полной дисфункции этих клеток [16,19].…”