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Abstract

In the present study, we examined the effect of Silibinin on the accumulation of oxidative stress induced by MPTP by in-vitro. After decapitation, healthy rat brain was removed rapidly from the skull and rinsed with cold artificial cerebrospinal fluid (ACSF) which has been equilibrated with 95% O 2 / 5% CO 2 gas mixture. Group I brain was incubated in CSF serve as normal, Group II lenses brain was incubated in CSF and DMSO (10%) serve as disease, Group III brain was incubated with MPTP (1 ng/mL) Group IV brain was incubated in MPTP with bromocriptine (10 µg/mL), Group V brain was incubated in MPTP with Silibinin (10 µg/mL) Group VI brain was incubated in MPTP with Silibinin (20 µg/mL). After 1 h of incubation brain slices were homogenized in PBS buffer, pH 7.4 and estimated for protein content, LPO* and GSH as per the procedures. MPTP incubated brain LPO* levels were increased, and GSH activity decreased compared to normal group. Silibinin treated group brain LPO* levels were significantly decreased and GSH activity was increased. The research results were concluded that the Silibinin exhibited significant neuroprotective effect against MPP + free radicals due to their antioxidant activity.

Predicting Antioxidant, Neuroprotective, and Toxicity Activity of Murraya koenigii Essential Oil by in silico and ex vivo against Rotenone-induced Model

“…They were incubated at 37 • C in ACSF at pH 7.4 and gassed with 95% O 2 /CO 2 for 1hr. each slice was differentiated into groups (7) . Group I served as control treated with normal saline, Group II with DMSO, Group III as rotenone group 1ng/ml, standard group slice was treated with Syndopa, and another 3 groups IV, V, and VI are pre-treated with M.koeingii oil (10µL, 20µL, 40µL).…”