Untitled

Abstract

The regional energy status and the availability of metabolic substrates during brain development are important, since a variety of fetal metabolic insults have been increasingly implicated in the evolution of neonatal brain disorders. The response of the brain to a metabolic insult is determined, in large part, by the ability to utilize the various substrates for intermediary metabolism in order to maintain energy stores within the tissue. To ascertain if metabolic conditions of the fetal brain make it more or less vulnerable to a stress, the high-energy phosphates and glucose-related compounds were examined in five regions of the embryonic day 18 (E-18) fetal brain. Glucose and glycogen levels in the E-18 fetal brain were generally higher in the cerebellum and its neuroepithelium than in the hippocampus, cerebral cortex, and its neuroepithelium. Regional lactate and high-energy phosphate concentrations were essentially the same in the five regions. Subsequently, the metabolic profile was examined in the cerebral cortex and striatum from E-18, postpartum day 7 (P-7) and adult rats. At the various stages of development, there were only minimal differences in the high-energy phosphate levels in the striatum and cortex. Glucose levels, the primary substrate in the adult brain, were essentially unchanged throughout development. In contrast, lactate was significantly elevated by 6- and 2-fold over those in the adult brain in the E-18 and P-7 striatum and cortex, respectively. Another alternative substrate, beta-hydroxybutyrate, was also significantly elevated at E-18 and increased more than 2-fold at P-7, but was barely detectable in the adult cortex and striatum. Finally, glucose and lactate levels were examined in cerebrospinal fluid, blood, and brain from the E-18 brain to determine if a gradient among the compartments exists. The levels of both lactate and glucose exhibited a concentration gradient in the E-18 fetus: blood > cerebrospinal fluid > brain parenchyma. The results indicate that energy state in the fetal brain is comparable to that in the neonates and the adults, but that the availability of alternative substrates for intermediary metabolism change markedly with development. The age-dependent substrate specificity for intermediary metabolism could affect the response of the fetal brain to a metabolic insult.

Glucocorticoids and Preterm Hypoxic-Ischemic Brain Injury: The Good and the Bad

“…Given the frighteningly limited information on whether glucocorticoids protect or sensitise to HI brain injury in preterm fetuses [130] it is of considerable concern that glucocorticoids have been suggested to be potential perinatal neuroprotection agents [84, 85]. The recent finding discussed above that a clinical course of maternal dexamethasone exposure triggers major changes in brain activity, including epileptiform events now raises the possibility that maternal glucocorticoids may well be both protective and damaging, depending on the precise timing before the preterm fetus and infant are exposed to an HI insult.…”

The metamorphosis of the developing cerebellar microcircuit

“…The crux of this argument is that the typical experimental artificial cerebrospinal fluid (aCSF) used in in vitro experiments is based on adult brain CSF, which may not mimic the CSF of the young mouse brain well. Neonatal brains contain elevated levels of several metabolic substrates, in particular the ketone body β-hydroxybutyrate (BHB) compared to adult brains [19]. These two studies tested what effect elevated BHB in juvenile brain might play on E GABA during development.…”

Enantioselective determination of 3-hydroxybutyrate in the tissues of normal and streptozotocin-induced diabetic rats of different ages

“…To better understand the physiological role of the minor enantiomer, l-3HB, in maturation and development, we employed rats of different ages to observe age-dependent alterations, because postnatal changes in the utilization of ketone bodies or glucose have been reported [9].…”