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Abir, Ronit; Fisch, Benjamin; Raz, Ahud; Nitke, Shmuel; Ben‐Rafael, Zion

doi:10.1023/a:1022578303272

Cited by 44 publications

(3 citation statements)

References 77 publications

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“…Human isolated primordial follicles do not grow properly in culture. 73,74 Indeed, despite the encouraging results achieved by Hovatta, 75 it has not yet been possible to grow human isolated primordial follicles to the mature oocyte stage. This led us to consider an alternative strategy, which involves grafting isolated ovarian follicles.…”

Section: Isolated Preantral Folliclesmentioning

confidence: 99%

Advances in Fertility Preservation in Young Female Cancer Patients

Dolmans¹,

Donnez²

2008

European Endocrinology

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Section: Isolated Preantral Folliclesmentioning

confidence: 99%

Advances in Fertility Preservation in Young Female Cancer Patients

Dolmans¹,

Donnez²

2008

European Endocrinology

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“…Advancements in cancer treatment continue to improve survival and cure rates in women of reproductive age. Many, however, will struggle with ovarian failure and premature menopause as a consequence of potentially gonadotoxic chemotherapy and radiation [ 1 ]. Among the options currently available for fertility preservation in these patients is cryopreservation and future auto-transplantation of ovarian cortical tissue containing immature follicles [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Activated ovarian endothelial cells promote early follicular development and survival

et al. 2017

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BackgroundNew data suggests that endothelial cells (ECs) elaborate essential “angiocrine factors”. The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development.MethodsMouse ovarian ECs were isolated using magnetic cell sorting or by FACS and cultured in serum free media. After a constitutive activation of the Akt pathway was initiated, early follicles (50–150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) (n = 32), gel (n = 24) or within matrigel (n = 27) in serum free media for 14 days. Follicular growth, survival and function were assessed.ResultsAfter 6 passages, flow cytometry showed 93% of cells grown in serum-free culture were VE-cadherin positive, CD-31 positive and CD 45 negative, matching the known EC profile. Beginning on day 4 of culture, we observed significantly higher follicular and oocyte growth rates in follicles co-cultured with AOECs compared with follicles on gel or matrigel. After 14 days of culture, 73% of primary follicles and 83% of secondary follicles co-cultured with AOEC survived, whereas the majority of follicles cultured on gel or matrigel underwent atresia.ConclusionsThis is the first report of successful isolation and culture of ovarian ECs. We suggest that co-culture with activated ovarian ECs promotes early follicular development and survival. This model is a novel platform for the in vitro maturation of early follicles and for the future exploration of endothelial-follicular communication.CapsuleIn vitro development of early follicles necessitates a complex interplay of growth factors and signals required for development. Endothelial cells (ECs) may elaborate essential “angiocrine factors” involved in organ regeneration. We demonstrate that co-culture with ovarian ECs enables culture of primary and early secondary mouse ovarian follicles.

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“…To date, various efforts have been made to preserve ovarian function and fertility, for example, administration of gonadotropinreleasing hormone agonist prior to the initiation of chemotherapy, cryopreservation of embryos, mature oocytes, or ovarian tissue (Pereira et al, 2017). Although means of ovarian protections (Abir, Fisch, Raz, Nitke, & Ben-Rafael, 1998) were attempted to prevent or decrease the loss of fertility before the chemotherapy or radiotherapy Eun Cho and Yoon Young Kim contributed equally to this work. (Fisch & Abir, 2018), current treatments are still highly toxic to gonads such as ovary and testis.…”

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confidence: 99%

A new possibility in fertility preservation: The artificial ovary

Cho

Kim

Noh

et al. 2019

J Tissue Eng Regen Med

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Conventional fertility preservation methods such as oocyte or embryo cryopreservation are currently insufficient to treat including those patients with prepubertal cancer and premature ovarian failure. Ovarian tissue cryopreservation presents as an alternative but has limitations with a potential risk of reintroducing malignant cells in patients who recover from cancer, those of chemotherapy prior to tissue cryopreservation. The so called “artificial ovary” aims to resolve this issue by transplanting isolated follicles with or without a biological scaffold. The artificial ovary may also offer an effective alternative option for those who cannot benefit from traditional assisted reproductive techniques such as in vitro fertilisation. To date, in animal studies and human trial, the artificial ovary restored endocrine function, achieved in vivo follicular development, and resulted in successful pregnancies. However, development of a technique for higher follicular recovery rate and a more optimised design of delivery scaffold, better transplantation techniques to prevent postsurgical ischemia, and consideration for genetic safety are required for safer and consistent human clinical applications. Ideas from different transplantation surgeries (e.g., entire ovary, ovarian cortex, and transplantation with tissue‐engineered products) can be applied to enhance the efficacy of artificial ovarian transplantation. For the better application of artificial ovary, a deeper understanding of mechanical and biochemical properties of the ovary and folliculogenesis after cryopreservation, transplantation with or without scaffold, and development of sophisticated in vivo imaging techniques of transplanted artificial ovary need to precede its efficient clinical application.

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Abstract: Although women who undergo chemotherapy face limited options for fertility preservation, intensive studies in cryopreservation and in vitro maturation of follicles harbor hope for brighter prospects in the future.

Cited by 44 publications

References 77 publications

Advances in Fertility Preservation in Young Female Cancer Patients

Advances in Fertility Preservation in Young Female Cancer Patients

Activated ovarian endothelial cells promote early follicular development and survival

A new possibility in fertility preservation: The artificial ovary

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