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Lambé, Pascal; Mutambel, Hity S.N.; Deltour, R.; Dinant, Monique

doi:10.1023/a:1026402229460

Cited by 33 publications

(7 citation statements)

References 19 publications

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“…Genotype 7042-DMR showed greater callusing response than genotype 843B from all explants tested, indicating that genotype had significant influence on callus induction frequency. This result on genotypic variation is consistent with that reported by previous investigators in other genotypes of pearl millet (Lambe et al 1999) and in sorghum (Cai and Butler 1990;Guo and Liang 1993). Along with genotype, significant differences were also observed among various explants and 2,4-D levels tested; however, there was no significant interaction among these factors.…”

Section: Resultssupporting

confidence: 93%

“…4). Our results showed significant improvement in embryogenic callus induction when compared to earlier studies, such as Lambe et al (1999), which reported 12-45% induction of embryogenic callus from immature zygotic embryos and shoot tip explants, while Mythili et al (1997) reported 21-26% embryogenesis from immature inflorescence explants. Similar to earlier reports, we observed the production of translucent non-embryogenic callus consisting of hyperhydric cells (Vasil and Vasil 1981b;Lambe et al 1999), which never showed regeneration of somatic embryos.…”

Section: Resultssupporting

confidence: 47%

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In vitro plant regeneration through somatic embryogenesis and direct shoot organogenesis in Pennisetum glaucum (L.) R. Br.

Jha

Yadav

Anjaiah

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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An efficient in vitro plant regeneration protocol through somatic embryogenesis and direct shoot organogenesis has been developed for pearl millet (Pennisetum glaucum). Efficient plant regeneration is a prerequisite for a complete genetic transformation protocol. Shoot tips, immature inflorescences, and seeds of two genotypes (843B and 7042-DMR) of pearl millet formed callus when cultured on Murashige and Skoog (MS) medium supplemented with varying levels of 2,4-dichlorophenoxyacetic acid (2,4-D; 4.5, 9, 13.5, and 18 μM). The level of 2,4-D, the type of explant, and the genotype significantly effected callus induction. Calli from each of the three explant types developed somatic embryos on MS medium containing 2.22 μM 6-benzyladenine (BA) and either 1.13, 2.25, or 4.5 μM of 2,4-D. Somatic embryos developed from all three explants and generated shoots on MS medium containing high levels of BA (4.4, 8.8, or 13.2 μM) combined with 0.56 μM 2,4-D. The calli from the immature inflorescences exhibited the highest percentage of somatic embryogenesis and shoot regeneration. Moreover, these calli yielded the maximum number of differentiated shoots per callus. An efficient and direct shoot organogenesis protocol, without a visible, intervening callus stage, was successfully developed from shoot tip explants of both genotypes of pearl millet. Multiple shoots were induced on MS medium containing either BA or kinetin (4.4, 8.8, 17.6, or 26.4 μM). The number of shoots formed per shoot tip was significantly influenced by the level of cytokinin (BA/ kinetin) and genotype. Maximum rooting was induced in 1/2 strength MS with 0.8% activated charcoal. The regenerated plants were transferred to soil in pots, where they exhibited normal growth.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 47%

In vitro plant regeneration through somatic embryogenesis and direct shoot organogenesis in Pennisetum glaucum (L.) R. Br.

Jha

Yadav

Anjaiah

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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“…We hypothesize that this low recovery of transgenic lines is due to a decrease in plant regeneration potential after a long culture period. Longterm cultures are often reported to lead to decreased plant regeneration efficiency and increases in the number of albino plant regenerants (Cho et al 1998;Gondo et al 2005;Lambé et al 1998). Similar phenomenon has occurred within a 3-month culture term in napier grass (Chandler and Vasil 1984) and our data showed that we obtained a transgenic plant which was recovered from callus with the shortest culture term of 140 days (Table 4).…”

Section: Discussionsupporting

confidence: 83%

Plant regeneration from embryogenic callus derived from shoot apices and production of transgenic plants by particle inflow gun in dwarf napier grass (<i>Pennisetum purpureum</i> Schumach.)

Gondo

Umami

Muguerza

et al. 2017

Plant Biotechnology

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Napier grass (Pennisetum purpureum Schumach.) is a highly productive C4 tropical forage grass that has been targeted as a potential bioenergy crop. To further increase the efficiency of bioethanol production by molecular breeding, a reliable protocol for genetically transforming napier grass is essential. In this study, we report the creation of transgenic napier grass plants derived from embryogenic callus cultures of shoot apices. Embryogenic callus was initiated in three accessions of napier grass and a napier grass×pearl millet hybrid using Murashige and Skoog (MS) medium supplemented with 2.0 mg L −1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg L −1 6-benzylaminopurine (BAP) and 50 µM copper sulfate (CuSO 4 ). Of the accessions tested, a dwarf type with late-heading (DL line) had the best response for embryogenic callus formation. Highly regenerative calli that formed dense polyembryogenic clusters were selected as target tissues for transformation. A plasmid vector, pAHC25, containing an herbicide-resistance gene (bar) and the β-glucuronidase (GUS) reporter gene was used in particle bombardment experiments. Target tissues treated with 0.6 M osmoticum were bombarded, and transgenic plants were selected under 5.0 mg L −1 bialaphos selection. Although a total of 1400 target tissues yielded nine GUS-positive bialaphos-resistant calli, only one transgenic line that was derived from target tissue with the shortest culture term produced four transgenic plants. Thus, the length of time that the target tissue is in callus culture was one of the most important factors for acquiring transgenic plants in napier grass. This is the first report of successfully producing transgenic napier grass plants.

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“…A high regeneration ability (or morphogenetic potential) of callus tissue facilitates many biotechnological applications. However, the loss of morphogenetic potential is a common phenomenon that can occur soon after callus initiation or even as late as after many years of culture [14,15,16,17]. In any case, poor regeneration is a common problem that restricts the application of biotechnological manipulations in many species.…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential in Brachypodium Distachyon Callus Culture

Betekhtin

Rojek

Nowak

et al. 2018

IJMS

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Effective regeneration of callus tissue into embryos and then into whole plants is essential for plant biotechnology. The embryonic potential is often low and can further decrease with time in culture, which limits the utilisation of calli for transformation procedures and in vitro propagation. In this study, we show that the loss of embryogenic potential in callus cultures of Brachypodium distachyon is progressive over time. Flow cytometry analyses indicated endoploidy levels increased in 60- and 90-day-old calli with effective loss of the 2C DNA content peak in the latter. Analysis of indolic compounds content revealed a decrease in 60- and 90-day-old calli compared to either freshly isolated explants or 30-day-old calli. Immunohistochemical analysis revealed a decrease in arabinogalactan proteins (AGP) signal with the time of culture, but extensin (EXT) epitopes either increased (JIM12 epitopes) or decreased (JIM11 epitopes). The transcript accumulation levels of AGPs and EXTs confirmed these results, with most of AGP and EXT transcripts gradually decreasing. Some chimeric EXT transcripts significantly increased on the 30th day of culture, perhaps because of an increased embryogenic potential. Selected somatic embryogenesis-related genes and cyclins demonstrated a gradual decrease of transcript accumulation for YUCCA (YUC), AINTEGUMENTA-LIKE (AIL), BABY BOOM (BBM), and CLAVATA (CLV3) genes, as well as for most of the cyclins, starting from the 30th day of culture. Notably, WUSCHEL (WUS) transcript was detectable only on the 30th and 60th day and was not detectable in the zygotic embryos and in 90-day-old calli.

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Cited by 33 publications

References 19 publications

In vitro plant regeneration through somatic embryogenesis and direct shoot organogenesis in Pennisetum glaucum (L.) R. Br.

In vitro plant regeneration through somatic embryogenesis and direct shoot organogenesis in Pennisetum glaucum (L.) R. Br.

Plant regeneration from embryogenic callus derived from shoot apices and production of transgenic plants by particle inflow gun in dwarf napier grass (<i>Pennisetum purpureum</i> Schumach.)

Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential in Brachypodium Distachyon Callus Culture

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