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Galijatovic, Alema; Walle, U K; Walle, Thomas

doi:10.1023/a:1007506222436

Cited by 89 publications

(21 citation statements)

References 23 publications

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“…2,3,12) Induction of different isoforms of the UGT1A subfamily in Caco-2 cells by dietary flavonoids, such as chrysin and quercetin, have also been reported. 13) Although turmeric contains many phenolic compounds with antioxidant properties, curcumin might play an essential role in the induction of UGT1A genes as demonstrated in Fig. 6.…”

Section: Discussionmentioning

confidence: 99%

Turmeric and Curcumin Modulate the Conjugation of 1-Naphthol in Caco-2 Cells

Naganuma

Saruwatari

Okamura

et al. 2006

Biological & Pharmaceutical Bulletin

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Turmeric is the powdered dry rhizome of the plant Curcuma longa and has been widely used as a coloring agent, as a spice and has been utilized in the treatment of inflammatory conditions and other diseases.1) Curcumin is a yellow pigment and is the major anti-oxidant and anti-inflammatory constituent of turmeric. It is present in curry and mustard, and it is used extensively in Asian countries and in traditional medicines. Curcumin and/or turmeric have both been shown to possess cancer chemopreventive activity in addition to anti-inflammatory activity, and these compounds have thus generated considerable attention as alternative medicines in recent years.2-4) The low incidence of colon cancer in Asian countries could be related to low meat intake, but may also be due to the regular use of turmeric in the diet of these regions. As the colon is exposed to turmeric and curcumin, it is a likely target for the anticarcinogenic activity of these compounds. Moreover, since these agents are often administered in combination with conventional therapeutic drugs, it is very important to further explore the potential benefits of herb-drug interactions. Previously we have investigated such herb-drug interactions at the level of conjugation. 5,6) We have also recently established an assay system for conjugation reactions of 1-naphthol using a human adenocarcinoma cell line, Caco-2.7) In our current study, to elucidate the possible interaction of both turmeric and curcumin with the conjugation pathways in cells, which in many cases are involved in the activation of procarcinogens, we measured their effects on the conjugation activity of 1-naphthol in Caco-2 cells. MATERIALS AND METHODS MaterialsPowdered turmeric was obtained from Wako, Ltd. and curcumin was purchased from Sigma. Water-soluble and DMSO-soluble turmeric solutions were prepared by stirring turmeric powder (10 g) in either 100 ml distilled water or DMSO for 3 h at room temperature. Each solution was then filtered.Cell Culture Human adenocarcinoma Caco-2 cells were grown in 12-well plates in 1 ml minimal essential medium (MEM) supplemented with 10% fetal bovine serum and additional non-essential amino acids. The cells were seeded at 5ϫ10 5 cells/ml and cultured for up to 3 weeks with medium changes every 4-5 d.Analyses of 1-Naphthyl Sulfates and Glucuronide 1-Naphthol (200 mM) was added to the culture medium and the cells were further incubated at 37°C. Aliquots of 50 ml of medium were removed at various times, and 30 ml of each of these samples was then filtered and injected into a HPLC apparatus, equipped with an ODS column (Chromolith Performance RP-18e, 100ϫ4.6 mm, Merck). The mobile phase consisted of 2 mM tetrabutylammonium hydrogen sulfate in water and acetonitrile (65 : 35). The flow rate was 1.0 ml/min with a column temperature of 40°C and elution was monitored at 285 nm. The retention times for 1-naphthol, 1-naphthyl sulfate and 1-naphthyl glucuronide were determined to be 19.0 min, 17.6 min and 4.6 min, respectively. The effects of curcumin or turmeric o...

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Section: Discussionmentioning

confidence: 99%

Turmeric and Curcumin Modulate the Conjugation of 1-Naphthol in Caco-2 Cells

Naganuma

Saruwatari

Okamura

et al. 2006

Biological & Pharmaceutical Bulletin

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“…Briefly, the reaction mixture (50 ml) contained 100 mM Tris-HCl (pH 7.4), 10 mM MgCl 2 , 1 mM 1-naphthol and a microsome fraction (100 mg of protein). The reactions were initiated by the addition of 0.2 mM UDP-[U- 14 C]glucuronic acid (0.1 mCi) and continued for 30 min at 37°C. The reactions were stopped by the addition of 100 ml ethanol (95%), and the mixtures were then centrifuged at 1000 g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…14,15) Coffee also contains several phenolic compounds with antioxidant properties, and its total polyphenol content has been reported to range from 200 to 550 mg per serving. 16,17) Such phenolic antioxidant compounds in coffee may induce UGT1A6 expression in Caco-2 cells, but no induction of UGT1A1 or UGT1A6 genes was observed in our experiments.…”

Section: Effects Of Coffee On Conjugation Reactions In Caco-2 Cellsmentioning

confidence: 99%

The Effects of Coffee on Conjugation Reactions in Human Colon Carcinoma Cells

Okamura

Suzuki

Yanase

et al. 2005

Biological & Pharmaceutical Bulletin

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We examined the effect of coffee on conjugation reactions in the human colon carcinoma cell line, Caco-2. After supplementing Caco-2 cultures with both 1-naphthol (200 m mM) and various concentrations of coffee, the accumulation of 1-naphthyl sulfate and glucuronide in the growth medium was determined by analytical HPLC over a 24-h period. A strong reduction in sulfo-conjugation (Ͻ50% of the control value) was observed in cells treated with coffee (IC 50 ‫,)%3.4؍‬ but no effect on glucuronic acid conjugation (glucuronidation) was observed. Coffee was also found to inhibit sulfotransferase (SULT) activity towards 1-naphthol in vitro to a similar extent (IC 50 ‫)%1.5؍‬ as in intact Caco-2 cells, but exhibited no effect upon UDP-glucuronosyl transferase (UGT) activity in vitro. PCR analyses showed no significant changes in the expression of either SULT genes (SULT1A1 and SULT1A3) or UGT genes (UGT1A1 and UGT1A6) following treatment with coffee solutions of up to 5% in concentration. These results suggest that the consumption of coffee can modify sulfo-conjugation reactions within intestinal epithelial cells, which may possibly affect the bioavailability of therapeutic drugs and the toxicity of environmental chemicals.

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“…[17][18][19] If these events occur in the placenta, this might interfere with the normal fetal growth and development and TR-TBT cells may thus be useful in future studies to analyze the effects of such exposures on drug metabolizing enzymes in the placenta.…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling of Drug Metabolizing Enzymes in Rat Syncytiotrophoblast Cell Lines

Ikeda

Nakashima

Tamura

2006

JOURNAL OF HEALTH SCIENCE

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TR-TBT-18d-1 and -18d-2 are syncytiotrophoblast cell lines established from the placenta of a transgenic rat harboring a temperature-sensitive simian virus 40 large T-antigen (Tg-rat). To explore the functional roles of drug metabolizing enzymes in the syncytiotrophoblasts of the placenta, we analyzed the gene expression of both cytochrome P450 (CYP) and phase II conjugation enzymes in these TR-TBT cell lines. A relatively high level of expression of CYP1A1 and CYP2C11 was detectable in the 18d-1 cells whereas CYP2E1 was more strongly expressed in the 18d-2 cells. A moderate degree of expression of CYP2B2 and CYP3A, and low expression of CYP1A2, was observed in both cell lines, but no CYP3A2 was detectable in either. Relatively high levels of SULT2 family gene expression were also evident in these TR-TBT cells, although the SULT1 family genes were found to be either absent or present at only moderate levels. Strong expression of both the UGT1 and UGT2 subfamily, and also the glutathione S-transferase (GST) subfamily, was also observed in both cell lines. These results suggest that TR-TBT cells are a useful model system for the study of syncytiotrophoblasts, which act as a protective fetal barrier against harmful xenobiotics.

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Abstract: Diet-mediated induction of intestinal UGT may be important for the bioavailability of carcinogens and other toxic chemicals as well as therapeutic drugs.

Cited by 89 publications

References 23 publications

Turmeric and Curcumin Modulate the Conjugation of 1-Naphthol in Caco-2 Cells

Turmeric and Curcumin Modulate the Conjugation of 1-Naphthol in Caco-2 Cells

The Effects of Coffee on Conjugation Reactions in Human Colon Carcinoma Cells

Expression Profiling of Drug Metabolizing Enzymes in Rat Syncytiotrophoblast Cell Lines

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