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Vancha, Ajith R.; Govindaraju, Suman; Parsa, Kishore V. L.; Jasti, Madhuri; González-Garcı́a, Maribel; Ballestero, Rafael P.

doi:10.1186/1472-6750-4-23

Cited by 177 publications

(87 citation statements)

References 26 publications

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“…3b, no hemolysis of red blood cells was caused by the PDs, even at a concentration of 4000 μg/mL, indicating favorable blood compatibility. Thus, the cellular experiments and hemolysis assay confirmed the biocompatibilities of the PDs, especially in comparison with PEI alone, the cytotoxicity of which has been cited as a significant concern in the literature26.…”

Section: Resultssupporting

confidence: 57%

Ultrabright and Multicolorful Fluorescence of Amphiphilic Polyethyleneimine Polymer Dots for Efficiently Combined Imaging and Therapy

Sun

Cao

et al. 2013

Sci Rep

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Multifunctional nanoparticles as theranostic tools hold great potential for its unique and efficient way to visualize the process of disease treatment. However, the toxicity of conventional fluorescent labels and difficulty of functionalization limit their widespread use. Recently, a number of amino-rich polymers have demonstrated high luminescent fluorescence but rarely showed potential for in vivo imaging due to their blue fluorescence. Here, a general route has been found to construct polymer-based multifunctional nanoparticles for combined imaging and drug delivering. The weak fluorescent polyethyleneimine (PEI) has been conjugated with hydrophobic polylactide as the amphiphilic PEI for construction of nanoparticles which showed bright and multicolor fluorescence with high drug loading capacity. The paclitaxel-loaded nanoparticles showed significant therapy effect in contrast to the free paclitaxel. Meanwhile, fluorescence imaging of the nanoparticles showed accumulation around tumor. These results demonstrate a new type of polymer-based multifunctional nanoparticles for imaging-guided drug delivery.

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Section: Resultssupporting

confidence: 57%

Ultrabright and Multicolorful Fluorescence of Amphiphilic Polyethyleneimine Polymer Dots for Efficiently Combined Imaging and Therapy

Sun

Cao

et al. 2013

Sci Rep

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“…We tested the cytocompatibility of PELD using an established method for polycations with the fibroblast cell line, NIH 3T3 [24,25]. We compared PELD to PEI, a common polycation used in cell culture and as a polymer transfectant for non-viral gene delivery [26,27]. We also used PEAD as a positive control, as we have previously shown it to be highly biocompatible both in vitro and in vivo [17].…”

Section: Resultsmentioning

confidence: 99%

Lysine-based polycation:heparin coacervate for controlled protein delivery

2014

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Polycations have good potential as carriers of proteins and genetic material. However, poor control over the release rate and safety issues currently limit their use as delivery vehicles. Here we introduce a new lysine-based polycation, poly(ethylene lysinylaspartate diglyceride) (PELD), which exhibits high cytocompatibility. PELD self-assembles with the biological polyanion heparin into a coacervate that incorporates proteins with high loading efficiency. Coacervates of varying surface charge were obtained by simple alteration of the PELD:heparin ratio and resulted in diverse release profiles of the model protein bovine serum albumin. Therefore, coacervate charge represents a direct means of control over release rate and duration. The PELD coacervate also rapidly adsorbed onto a porous polymeric scaffold, demonstrating potential use in tissue engineering applications. This coacervate represents a safe and tunable protein delivery system for biomedical applications.

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“…Cells were transiently transfected with polyethyleneamine as described48. N2a cells with PGRN deletion were generated by infecting N2a cells with lentivirus expressing Cas9 only or Cas9 and guide RNA targeted to mouse Grn (oligos with 5′-CACCGCGGACCCCGACGCAGGTAGG-3′ and 5′-AAACCCTACCTGCGTCGGGGTCCGC-3′ were ligated to pLenti-CRISPR (Addgene)).…”

Section: Methodsmentioning

confidence: 99%

Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations

et al. 2017

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Haploinsufficiency of progranulin (PGRN) due to mutations in the granulin (GRN) gene causes frontotemporal lobar degeneration (FTLD), and complete loss of PGRN leads to a lysosomal storage disorder, neuronal ceroid lipofuscinosis (NCL). Accumulating evidence suggests that PGRN is essential for proper lysosomal function, but the precise mechanisms involved are not known. Here, we show that PGRN facilitates neuronal uptake and lysosomal delivery of prosaposin (PSAP), the precursor of saposin peptides that are essential for lysosomal glycosphingolipid degradation. We found reduced levels of PSAP in neurons both in mice deficient in PGRN and in human samples from FTLD patients due to GRN mutations. Furthermore, mice with reduced PSAP expression demonstrated FTLD-like pathology and behavioural changes. Thus, our data demonstrate a role of PGRN in PSAP lysosomal trafficking and suggest that impaired lysosomal trafficking of PSAP is an underlying disease mechanism for NCL and FTLD due to GRN mutations.

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Untitled

Cited by 177 publications

References 26 publications

Ultrabright and Multicolorful Fluorescence of Amphiphilic Polyethyleneimine Polymer Dots for Efficiently Combined Imaging and Therapy

Ultrabright and Multicolorful Fluorescence of Amphiphilic Polyethyleneimine Polymer Dots for Efficiently Combined Imaging and Therapy

Lysine-based polycation:heparin coacervate for controlled protein delivery

Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations

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