The ASACUSA collaboration has carried out laser spectroscopy of an antiprotonic helium atom (pHe + : a Coulomb three-body system consisted of an antiproton, electron, and a helium nucleus) at CERN's Antiproton Decelerator facility and measured transition frequencies of pHe + . By comparing the measured frequencies with three-body QED calculations, the antiprotonto-electron mass ratio mp/m e was determined. We recently developed a new laser system consisting of single-mode continuous-wave lasers, a femtosecond optical frequency comb, a pulse-amplified dye laser, and a chirp compensation, and achieved a precision of ∼ 1 × 10 −8 on thepHe + transition frequencies, which was about one order of magnitude improvement. The comparison of the measured frequencies and the three-body QED calculations yielded the antiproton-to-electron mass ratio of mp/m e = 1836.152 674(5). Combined with a constraint on the proton and antiproton charge-tomass ratio of the TRAP group, a limit on any CPT-violating difference between the antiproton and proton charges and masses was also set as |Q p + Qp|/Q p ∼ |m p − mp|/m p < 2 × 10 −9 at a confidence level of 90 %.