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Wicker, Emmanuel; Moussart, Anne; Duparque, M.; Rouxel, Francis

doi:10.1023/a:1022020312157

Cited by 47 publications

(16 citation statements)

References 23 publications

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“…We selected strains with varied geographical origins and distinct host-spectra within legumes (Table 2). The RB84 strain, as the reference ATCC201684 strain, was isolated in Europe from pea fields and belongs to pathotype I according to pathogenicity tests performed with various genotypes of pea [64,65]. Both strains have a broad spectrum of hosts within the legumes but have a preference for pea [66].…”

Section: Illumina Sequencing Of Four Strains Of a Euteiches With Diff...mentioning

confidence: 99%

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

Kiselev

Clemente

Camborde

et al. 2022

JoF

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The soil-borne oomycete pathogen Aphanomyces euteiches causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of A. euteiches have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of A. euteiches ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of A. euteiches is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of A. euteiches with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of A. euteiches with legumes.

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Section: Illumina Sequencing Of Four Strains Of a Euteiches With Diff...mentioning

confidence: 99%

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

Kiselev

Clemente

Camborde

et al. 2022

JoF

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“…Differences between lines were determined by analysis of variance using the PROC GLM module of SAS detailed by Hamon et al (2011). To combine the disease severity scores of each RIL from different environments, different rating criteria (AGI1, AGI2, and RRI) and different scoring systems, the score least squares means were normalized with the formula: (Steel and Torrie, 1980), where x ij is the disease score of the ith entry in the jth environment, j X is the grand mean of all entries in the jth environment, and σ j is the standard deviation for the jth environment. For each RIL, a disease severity index (DSI) was then calculated as ( ) When the global index was used, the lines selected for release had higher levels of partial resistance to Aphanomyces root rot than either parent.…”

Section: Characteristicsmentioning

confidence: 99%

“…referred to as partial resistance (Lewis and Gritton, 1992). Although true races of A. euteiches have not been identifi ed in pea, degrees of difference in isolate aggressiveness have been well documented by Wicker and Rouxel (2001) using a set of differential lines (Wicker et al, 2003). Globally, 11 pathotypes have been characterized, and isolates from France tend to be more aggressive than isolates from Scandinavia, North America, or New Zealand .…”

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confidence: 99%

“…Globally, 11 pathotypes have been characterized, and isolates from France tend to be more aggressive than isolates from Scandinavia, North America, or New Zealand . Pathotypes I and III are found in the United States, whereas France is dominated by pathotype I Wicker et al, 2003). Studies to detect quantitative trait loci (QTL) associated with improved resistance to Aphanomyces root rot (Pilet-Nayel et al, 2002, 2005Hamon et al, 2011) have also identifi ed highly resistant germplasm lines from different recombinant inbred line (RIL) populations.…”

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confidence: 99%

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Registration of Pea Germplasm Lines Partially Resistant to Aphanomyces Root Rot for Breeding Fresh or Freezer Pea and Dry Pea Types

McGee

Coyne

Pilet‐Nayel

et al. 2012

J of Plant Registrations

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Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699International audienceEight germplasm lines of green pea (Pisum sativum L.)-RIL 846-07 (Reg. No. GP-99, PI 660729), RIL 847-08 (Reg. No. GP-100, PI 660730), RIL 847-22 (Reg. No. GP-101, PI 660731), RIL 847-28 (Reg. No. GP-102, PI 660732), RIL 847-45 (Reg. No. GP-103, PI 660733), RIL 847-50 (Reg. No. GP-104, PI 660734), RIL 847-53 (Reg. No. GP-105, PI 660735), and RIL 847-68 (Reg. No. GP-106, PI 660736)-were developed and released jointly by the USDA-ARS, the French National Institute for Agricultural Research (INRA), and North Dakota State University. They were selected from an F-8-derived recombinant inbred line (RIL) population developed from a cross between 'Dark Skin Perfection' and 90-2131. These lines all have high levels of partial resistance to root rot caused by Aphanomyces euteiches Drechs. f. sp. pisi when screened in 12 environments of 10 infested field nurseries across 4 yr and four locations in the USA and France and with two reference isolates in controlled conditions. On the basis of a disease index derived from the 12 environments (location-years), these lines had improved partial resistance compared with either parent. RIL 847-028, RIL 847-050, RIL 847-053, and RIL 847-068 performed better in U.S. field nurseries than in the French nurseries. Conversely, RIL 846-007, RIL 847-008, RIL 847-022, and RIL 847-045 performed better in the French field nurseries than in the U.S. nurseries. The eight lines also have acceptable agronomic characteristics: they have white flowers, straight blunt green pods, and green cotyledons; are clear seeded and semidwarf; and flower at the 14th-16th node in 57-61 d. The release of these germplasms will assist the breeding and development of either smooth-or wrinkle-seeded cultivars for the fresh or freezer pea and dry pea markets, respectively, with improved partial resistance to Aphanomyces root rot

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“…(2020) . Historically, breeding for resistance towards aphanomyces root rot has been most successful combining results from plant-pathogen interactions in both growth chambers and field experiments ( Moussart et al., 2001 ; Wicker et al., 2003 ; Pilet-Nayel et al., 2005 ; Abdullah et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of pea genotype PI180693 partial resistance towards aphanomyces root rot in commercial pea breeding

Kälin

Brantestam²,

Arvidsson³

et al. 2023

Front. Plant Sci.

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The cultivation of vining pea (Pisum sativum) faces a major constraint with root rot diseases, caused by a complex of soil-borne pathogens including the oomycetes Aphanomyces euteiches and Phytophtora pisi. Disease resistant commercial varieties are lacking but the landrace PI180693 is used as a source of partial resistance in ongoing pea breeding programs. In this study, the level of resistance and their interaction with A. euteiches virulence levels of six new back-crossed pea breeding lines, deriving from the cross between the susceptible commercial cultivar Linnea and PI180693, were evaluated for their resistance towards aphanomyces root rot in growth chamber and green house tests. Resistance towards mixed infections by A. euteiches and P. pisi and commercial production traits were evaluated in field trials. In growth chamber trials, pathogen virulence levels had a significant effect on plant resistance, as resistance was more consistent against A. euteiches strains exhibiting high or intermediate virulence compared with lowly virulent strains. In fact, line Z1701-1 showed to be significantly more resistant than both parents when inoculated with a lowly virulent strain. In two separate field trials in 2020, all six breeding lines performed equally well as the resistant parent PI180693 at sites only containing A. euteiches, as there were no differences in disease index. In mixed infections, PI180693 exhibited significantly lower disease index scores than Linnea. However, breeding lines displayed higher disease index scores compared with PI180693, indicating higher susceptibility towards P. pisi. Data on seedling emergence from the same field trials suggested that PI180693 was particularly sensitive towards seed decay/damping off disease caused by P. pisi. Furthermore, the breeding lines performed equally well as Linnea in traits important for green pea production, again emphasizing the commercial potential. In summary, we show that the resistance from PI180693 interacts with virulence levels of the pathogen A. euteiches and is less effective towards root rot caused by P. pisi. Our results show the potential use of combining PI180693 partial resistance against aphanomyces root rot with commercially favorable breeding traits in commercial breeding programs.

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Untitled

Cited by 47 publications

References 23 publications

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

Registration of Pea Germplasm Lines Partially Resistant to Aphanomyces Root Rot for Breeding Fresh or Freezer Pea and Dry Pea Types

Evaluation of pea genotype PI180693 partial resistance towards aphanomyces root rot in commercial pea breeding

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