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Kalamajka, Rainer; Hahnen, Silke; Cavalar, Markus; Töpsch, Sonja; Weier, Dagmar; Peterhänsel, Christoph

doi:10.1023/a:1024843819307

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…Nucleosome density appears to respond mainly to stronger promoter inactivation down to the lowest levels observed in leaves. This is reminiscent of the light-induced enhancement in chromatin accessibility to the restriction enzymes that we observed in an earlier study [16]. …”

Section: Resultssupporting

confidence: 68%

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Core promoter acetylation is not required for high transcription from the phosphoenolpyruvate carboxylase promoter in maize

Horst

Offermann

Dreesen

et al. 2009

Epigenetics & Chromatin

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BackgroundAcetylation of promoter nucleosomes is tightly correlated and mechanistically linked to gene activity. However, transcription is not necessary for promoter acetylation. It seems, therefore, that external and endogenous stimuli control histone acetylation and by this contribute to gene regulation. Photosynthetic genes in plants are excellent models with which to study the connection between stimuli and chromatin modifications because these genes are strongly expressed and regulated by multiple stimuli that are easily manipulated. We have previously shown that acetylation of specific histone lysine residues on the photosynthetic phosphoenolpyruvate carboxylase (Pepc) promoter in maize is controlled by light and is independent of other stimuli or gene activity. Acetylation of upstream promoter regions responds to a set of other stimuli which include the nutrient availability of the plant. Here, we have extended these studies by analysing histone acetylation during the diurnal and circadian rhythm of the plant.ResultsWe show that histone acetylation of individual lysine residues is removed from the core promoter before the end of the illumination period which is an indication that light is not the only factor influencing core promoter acetylation. Deacetylation is accompanied by a decrease in gene activity. Pharmacological inhibition of histone deacetylation is not sufficient to prevent transcriptional repression, indicating that deacetylation is not controlling diurnal gene regulation. Variation of the Pepc promoter activity during the day is controlled by the circadian oscillator as it is maintained under constant illumination for at least 3 days. During this period, light-induced changes in histone acetylation are completely removed from the core promoter, although the light stimulus is continuously applied. However, acetylation of most sites on upstream promoter elements follows the circadian rhythm.ConclusionOur results suggest a central role of upstream promoter acetylation in the quantitative regulation of gene expression in this model gene. Induced core promoter acetylation is dispensable for the highest gene expression in the diurnal and circadian rhythm.

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Section: Resultssupporting

confidence: 68%

“…The Actin -1 promoter was described in [15]. The Zein gene sequence was derived from Genbank accession gi 535019 [16]. …”

Section: Methodsmentioning

confidence: 99%

Core promoter acetylation is not required for high transcription from the phosphoenolpyruvate carboxylase promoter in maize

Horst

Offermann

Dreesen

et al. 2009

Epigenetics & Chromatin

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Analysis of Chromatin Structure in the Control Regions of the Chlamydomonas HSP70A and RBCS2 Genes

Lodha

Schroda

2005

Plant Mol Biol

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We have used DNaseI and micrococcal nuclease sensitivity assays to determine the chromatin structures in the control regions of the Chlamydomonas reinhardtii HSP70A and RBCS2 genes. Both genes appear to be organized into nucleosome arrays, which exhibit shorter nucleosome repeat lengths than bulk chromatin. In HSP70A we have identified up to four confined DNaseI hypersensitive sites, three of them localize to the promoter region, a fourth one to the fourth intron. Three hypersensitive sites map close to putative heat shock elements, one close to a CCAAT-box. All hypersensitive sites are located to internucleosomal linkers. Alternative nucleosome positions at half-nucleosomal phasing were constitutively detected in the HSP70A promoter region, indicating local chromatin remodelling. Upon heat shock, dramatic changes in the nucleosome structure of HSP70A were detected that particularly affected the promoter, but also a region within the fourth intron. In contrast, light induction entailed no change in HSP70A chromatin. In the RBCS2 control region we identified a strong DNaseI hypersensitive site that maps close to a CCAAT-box. This site forms the boundary of a nucleosome array with a region of approximately 700 bp apparently devoid of nucleosomes. This study demonstrates that chromatin structure may be determined readily at fairly high resolution in Chlamydomonas, suggesting this organism as a well-suited model for studying the role of chromatin structure on gene expression in photosynthetic eukaryotes.

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A drastic reduction in DOF1 transcript levels does not affect C4-specific gene expression in maize

Cavalar

Phlippen

Kreuzaler

et al. 2007

Journal of Plant Physiology

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Untitled

Cited by 8 publications

References 28 publications

Core promoter acetylation is not required for high transcription from the phosphoenolpyruvate carboxylase promoter in maize

Core promoter acetylation is not required for high transcription from the phosphoenolpyruvate carboxylase promoter in maize

Analysis of Chromatin Structure in the Control Regions of the Chlamydomonas HSP70A and RBCS2 Genes

A drastic reduction in DOF1 transcript levels does not affect C4-specific gene expression in maize

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