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De-yu, Xie; Hong, Yan

doi:10.1023/a:1010632619342

Cited by 53 publications

(12 citation statements)

References 14 publications

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“…However, unlike leaf-derived calli, these calli could not form adventitious buds on the same medium tested (data not shown), indicating differential responses of stem-derived calli and leaf-derived calli to the same medium. This observation differs from our previous report on Acacia mangium tissue culture, in which stem-derived calli could form adventitious bud-like structures just like the leaf-derived calli (Xie and Hong 2001).…”

Section: Discussioncontrasting

confidence: 99%

“…The elongation of the adventitious buds GA 3 is widely used to promote elongation of adventitious shoots in plant regeneration, e.g., for in vitro shoot proliferation of cassava (Bhagwat et al 1996) and regeneration of Acacia mangium via organogenesis (Xie and Hong 2001). In this study, the elongation of the adventitious buds was very slow on the medium containing 4.44 lM BAP and 0.54 lM NAA.…”

Section: Induction Of Calli and Adventitious Budsmentioning

confidence: 69%

“…In this study, the elongation of the adventitious buds was very slow on the medium containing 4.44 lM BAP and 0.54 lM NAA. During 2 months of subculture on this medium, no adventitious bud could grow longer than (Xie and Hong 2001), we added 1.45 lM of GA 3 into this medium. All adventitious buds (0.5-1.0 cm in length) tested in this study grew up to 2-4 cm long within just 3 weeks.…”

Section: Induction Of Calli and Adventitious Budsmentioning

confidence: 99%

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Shoot regeneration of dwarf dogwood (Cornus canadensis L.) and morphological characterization of the regenerated plants

Feng

Zhou

et al. 2009

Plant Cell Tiss Organ Cult

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Dwarf dogwoods (or the bunchberries) are the only suffrutex in Cornaceae. They are attractive ground cover ornamentals with clusters of small flowers surrounded by petaloid bracts. Little has been reported on plant regeneration of dogwoods. As a step toward unraveling the molecular basis of inflorescence evolution in Cornus, we report an efficient regeneration system for a dwarf dogwood species C. canadensis through organogenesis from rejuvenated leaves, and characterize the development of the plantlets. We used the nodal stem segments of vegetative branches as explants. Micropropogated shoots were quickly induced from axillary buds of nodes on an induction medium consisting of basal MS medium supplemented with 4.44 lM BAP and 0.54 lM NAA. The new leaves of adventitious shoots were used as explants to induce calli on the same induction medium. Nearly 65% of leaf explants produced calli, 80% of which formed adventitious buds. Gibberellic acid (1.45 lM) added to the same induction medium efficiently promoted quick elongation of most adventitious buds, and 0.49 lM IBA added to the basal MS medium promoted root formation from nearly 50% of the elongated shoots. The growth of plantlets in pot soil was characterized by the development of functional woody rhizomes, which continuously developed new aboveground vegetative branches, but not flowering branches, within the past 12 months. Potential reasons causing the delay of flowering of the regenerated plants are discussed. The establishment of this regeneration system facilitates developing a genetic transformation system to test candidate genes involved in the developmental divergence of inflorescences in Cornus.

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Section: Discussioncontrasting

confidence: 99%

Section: Induction Of Calli and Adventitious Budsmentioning

confidence: 69%

Section: Induction Of Calli and Adventitious Budsmentioning

confidence: 99%

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Shoot regeneration of dwarf dogwood (Cornus canadensis L.) and morphological characterization of the regenerated plants

Feng

Zhou

et al. 2009

Plant Cell Tiss Organ Cult

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“…& Fourr. (Kongbangkerd et al 2005), Cichorium intybus L. (Pieron et al 1993), and also for woody species like other species of poplar (McCown et al 1988), Eucalyptus grandis Hill ex Maid (Warrag et al 1991), Pinus radiata D. Don (Schestibratov et al 2003) and Acacia mangium Willd (Xie and Hong 2001). In the present work we report, for the first time, the histological study of the morphogenic events that give rise to adventitious shoot regeneration in P. euphratica leaf explants, through organogenic nodule formation on solid medium.…”

Section: Introductionmentioning

confidence: 60%

Morphogenesis induction and organogenic nodule differentiation in Populus euphratica Oliv. leaf explants

Ferreira

Batista

Serrazina

et al. 2008

Plant Cell Tiss Organ Cult

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Populus euphratica Oliv. is a deciduous poplar species, occurring mainly in riparian areas of China and Middle Eastern countries, and presenting high tolerance to extreme temperatures and high soil salinity. In this study, an optimized protocol for development and propagation of P. euphratica from leaf explants is reported, based on a morphogenic process that involves organogenic nodule differentiation. Adventitious shoot regeneration of P. euphratica from organogenic nodules of leaf explants was achieved within a range of concentrations of a-naphtalenacetic acid and 6-benzylaminopurine, at a fixed 2:1 ratio. Cambial cells started to divide 5 days after inoculation on culture medium and, after 12 days, several organizing centres were already formed. Non-friable callus tissue, together with organization centres, formed structures that evolved to nodules after about 40 days which were, then, able to regenerate new shoots after 50-60 days. The nodules did not separate from the mother explants and were able to successfully give rise to new adventitious shoots. These were rescued and successfully grown and rooted in different culture media, and fully developed plants were obtained. The regeneration system here described for P. euphratica is innovative, reproducible and data from histological studies of the morphogenic process support the classification of the regenerative structures as organogenic nodules.

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“…Cytokinins, auxins, and gibberellins are the three most commonly used plant growth regulators in plant tissue culture (Xie and Hong 2004, Ferreira et al 2008, Akram and Aftab 2009). An optimal combination of different types and concentrations of plant growth regulators is essential to induce competence in tissue for organogenesis and/or somatic embryogenesis (Wendling et al 2014).…”

Section: Introductionmentioning

confidence: 99%