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Roggero, P.; Dellavalle, G.; Ciuffo, M.; Pennazio, S.

doi:10.1023/a:1008742516820

Cited by 16 publications

(6 citation statements)

References 12 publications

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“…A triple antibody sandwich (TAS) ELISA was performed as previously described (25) for the detection of glycoproteins and nucleocapsids as an internal reference. Plates were coated with a mixture of polyclonal antisera against TSWV virions from the IVV collection (A292, A293, and A421), and after overnight incubation with samples, monoclonal antibody specific for N protein (4F2; DSMZ AS0106) or Gn protein (APO-2B3/2D11; IVV collection) was used.…”

Section: Methodsmentioning

confidence: 99%

The NSs Protein of Tomato spotted wilt virus Is Required for Persistent Infection and Transmission by Frankliniella occidentalis

Margaria¹,

Bosco

Vallino³

et al. 2014

J Virol

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Tomato spotted wilt virus (TSWV) is the type member of tospoviruses (genus Tospovirus), plant-infecting viruses that cause severe damage to ornamental and vegetable crops. Tospoviruses are transmitted by thrips in the circulative propagative mode. We generated a collection of NSs-defective TSWV isolates and showed that TSWV coding for truncated NSs protein could not be transmitted by Frankliniella occidentalis. Quantitative reverse transcription (RT)-PCR and immunostaining of individual insects detected the mutant virus in second-instar larvae and adult insects, demonstrating that insects could acquire and accumulate the NSs-defective virus. Nevertheless, adults carried a significantly lower viral load, resulting in the absence of transmission. Genome sequencing and analyses of reassortant isolates showed genetic evidence of the association between the loss of competence in transmission and the mutation in the NSs coding sequence. Our findings offer new insight into the TSWV-thrips interaction and Tospovirus pathogenesis and highlight, for the first time in the Bunyaviridae family, a major role for the S segment, and specifically for the NSs protein, in virulence and efficient infection in insect vector individuals. IMPORTANCEOur work is the first to show a role for the NSs protein in virus accumulation in the insect vector in the Bunyaviridae family: demonstration was obtained for the system TSWV-F. occidentalis, arguably one of the most damaging combination for vegetable crops. Genetic evidence of the involvement of the NSs protein in vector transmission was provided with multiple approaches.

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Section: Methodsmentioning

confidence: 99%

The NSs Protein of Tomato spotted wilt virus Is Required for Persistent Infection and Transmission by Frankliniella occidentalis

Margaria¹,

Bosco

Vallino³

et al. 2014

J Virol

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“…The effects of elevated temperatures on tospovirus symptomatology have shown to vary, depending on different combinations of tospoviruses and their host p species [56,57,62]. For example, the spread and symptom severity of GBNV in cow plants increased at higher temperatures (30 and 25 °C) compared to 20 and 15 °C [56 higher temperatures, the viral titer and RNA of GBNV, as well as hydrogen peroxide els, were increased in inoculated leaves (at 4 and 8 dpi) and systemic leaves (at 24 which resulted in severe necrosis [56].…”

Section: Discussionmentioning

confidence: 99%

“…The effects of elevated temperatures on tospovirus symptomatology have been shown to vary, depending on different combinations of tospoviruses and their host plant species [56,57,62]. For example, the spread and symptom severity of GBNV in cowpea plants increased at higher temperatures (30 and 25 • C) compared to 20 and 15 • C [56].…”

Section: Discussionmentioning

confidence: 99%

“…GBNV infection in cowpea only shows a severe necrosis at higher temperatures (30 • C and 25 • C) but not at 20 • C and 15 • C [56]. On the contrary, the systemic infection of impatiens necrotic spot virus (INSV) in capsicum plants (C. annuum, C. chinense PI152225, and C. chinense PI159236) was completely blocked when they were kept at a constant elevated temperature of 33 • C [57].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Elevated Temperature on the Susceptibility of Capsicum Plants to Capsicum Chlorosis Virus Infection

et al. 2022

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Capsicum, an important vegetable crop in Queensland, Australia, is vulnerable to both elevated temperatures and capsicum chlorosis virus (CaCV). Thus, it is imperative to understand the genetic responses of capsicum plants (Capsicum annuum) to CaCV under elevated temperature conditions. Here, we challenged susceptible plants (cv. Yolo Wonder) with CaCV and investigated the effects of elevated temperature on symptom expression, the accumulation of virus-derived short interfering RNA (vsiRNA) and viral RNA, and the expression of plant defense-associated genes. CaCV-inoculated plants initially showed more severe symptoms and higher viral concentrations at a higher temperature (HT, 35 °C) than at ambient temperature (AT, 25 °C). However, symptom recovery and reduced viral RNA accumulation were seen in the CaCV-infected plants grown at HT at later stages of infection. We also observed that HT enhanced the accumulation of vsiRNAs and that, concurrently, RNA interference (RNAi)-related genes, including Dicer-like2 (DCL2), DCL4, RNA-dependent RNA polymerase 1 (RdRp1), RdRp6, and Argonaute2 (AGO2), were upregulated early during infection. Moreover, continuous high levels of vsiRNAs were observed during later stages of CaCV infection at HT. Overall, our investigation suggests that HT facilitates CaCV replication during early infection stages. However, this appears to lead to an early onset of antiviral RNA silencing, resulting in a subsequent recovery from CaCV in systemic leaves.

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“…(Black et al, 1991;Boiteux & De Avila, 1994;Moury et al, 1997). Unfortunately, the Tsw gene is not effective against other tospovirus species which are in the same Asian group as TNRV including Groundnut ringspot virus (GRSV), Tomato chlorotic spot virus (TCSV) (Boiteux & De Avila, 1994), Impatiens necrotic spot virus (INSV) (Roggero et al, 1999) and CaCV (McMichael et al, 2002). Meanwhile, resistance to CaCV was found in C. chinense 'PI 290972', also giving a hypersensitive response and inherited as a single dominant gene (Persley et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Inheritance of Tomato Necrotic Ring Virus Resistance in Capsicum annuum

Puangmalai

Potapohn

Akarapisarn³

et al. 2013

JAS

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Tomato necrotic ring virus (TNRV) is a newly identified species of tospovirus in Thailand. The virus is widely spread and causes severe yield losses of vegetable crops such as tomato and pepper. Chemical control of the vector, thrips species, is very difficult, and the introduction of virus resistance would be a better approach to manage the disease.. In a previous study, two related Capsicum annuum lines, PY-6423 and PY-6424, were identified, which conferred medium resistance to TNRV. These two resistant lines were employed in an inheritance study in order to determine the number of genes controlling resistance in C. annuum. Therefore, the susceptible parent Early California wonder (ECW) was crossed with the resistant lines and F1 progenies were backcrossed to the susceptible and resistant parents as well as F2 were made. Evaluation of the F1, F2 and BC S and BC R indicated that the inheritance of the resistance fits a single recessive gene model.

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Cited by 16 publications

References 12 publications

The NSs Protein of Tomato spotted wilt virus Is Required for Persistent Infection and Transmission by Frankliniella occidentalis

The NSs Protein of Tomato spotted wilt virus Is Required for Persistent Infection and Transmission by Frankliniella occidentalis

Effects of Elevated Temperature on the Susceptibility of Capsicum Plants to Capsicum Chlorosis Virus Infection

Inheritance of Tomato Necrotic Ring Virus Resistance in Capsicum annuum

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