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doi:10.1111/jam.2008.105.issue-6

Cited by 6 publications

(1 citation statement)

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“…without differentiating the species, may be an ideal approach from a risk assessment point of view, specially focused on thermotolerant campylobacters (C. jejuni, C. coli, and C. lari). Many studies have successfully applied different primers targeting 16S rRNA, specific for genus Campylobacter and/or thermotolerant campylobacters (Botteldoorn et al, 2008;Josefsen, Cook, et al, 2004;Josefsen, Jacobsen, & Hoorfar, 2004;LeblancMaridor et al, 2011;Lubeck, Cook, Wagner, Fach, & Hoorfar, 2003;Lubeck, Wolffs, et al, 2003;Wolffs, Norling, Hoorfar, Griffiths, & Radstrom, 2005).…”

Section: Campylobacter Sppmentioning

confidence: 99%

Detection of foodborne pathogens by qPCR: A practical approach for food industry applications

Chapela

Garrido‐Maestu

2015

Cogent Food & Agriculture

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Microbiological analysis of food is an integrated part of microbial safety management in the food chain. Monitoring and controlling foodborne pathogens are traditionally carried out by conventional microbiological methods based on culture-dependent approaches in control laboratories and private companies. However, polymerase chain reaction (PCR) has revolutionized microbiological analysis allowing detection of pathogenic microorganisms in food, without the necessity of classical isolation and identification. However, at present, PCR and quantitative polymerase chain reaction (qPCR) are essential analytical tools for researchers working in the field of foodborne pathogens. This manuscript reviews recently described qPCR methods applied for foodborne bacteria detection, serving as economical, safe, and reliable alternatives for application in the food industry and control laboratories. Multiplex qPCR, which allows the simultaneous detection of more than one pathogen in one single reaction, saving considerable effort, time, and money, is emphasized in the article.

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Section: Campylobacter Sppmentioning

confidence: 99%

Detection of foodborne pathogens by qPCR: A practical approach for food industry applications

Chapela

Garrido‐Maestu

2015

Cogent Food & Agriculture

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Biological control of pre-harvest aflatoxin contamination in groundnut (Arachis hypogaeaL.) withBacillus subtilisG1

Shifa

Tasneem

Gopalakrishnan

et al. 2016

Archives of Phytopathology and Plant Protection

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Antimicrobial activity of four cationic peptides immobilised to poly-hydroxyethylmethacrylate

2016

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The objective of this study was to immobilise and characterise a variety of antimicrobial peptides (AMPs) onto poly-hydroxyethylmethacrylate (pHEMA) surfaces to achieve an antibacterial effect. Four AMPs, viz. LL-37, melimine, lactoferricin and Mel-4 were immobilised on pHEMA by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) which assisted covalent attachment. Increasing concentrations of AMPs were immobilised to determine the effect on the adhesion of Pseudomonas aeruginosa and Staphylococcus aureus. The AMP immobilised pHEMAs were characterised by X-ray photoelectron spectroscopy (XPS) to determine the surface elemental composition and by amino acid analysis to determine the total amount of AMP attached. In vitro cytotoxicity of the immobilised pHEMA samples to mouse L929 cells was investigated. Melimine and Mel-4 when immobilised at the highest concentrations showed 3.1 ± 0.6 log and 1.3 ± 0.2 log inhibition against P. aeruginosa, and 3.9 ± 0.6 log and 2.4 ± 0.5 log inhibition against S. aureus, respectively. Immobilisation of LL-37 resulted in up to 2.6 ± 1.0 log inhibition against only P. aeruginosa, but no activity against S. aureus. LFc attachment showed no antibacterial activity. Upon XPS analysis, immobilised melimine, LL-37, LFc and Mel-4 had 1.57 ± 0.38%, 1.13 ± 1.36%, 0.66 ± 0.47% and 0.73 ± 0.32% amide nitrogen attached to pHEMA compared to 0.12 ± 0.14% in the untreated controls. Amino acid analysis determined that the total amount of AMP attachment to pHEMA was 44.3 ± 7.4 nmol, 3.8 ± 0.2 nmol, 6.5 ± 0.6 nmol and 48.9 ± 2.3 nmol for the same peptides respectively. None of the AMP immobilised pHEMA surfaces showed any toxicity towards mouse L929 cells. The immobilisation of certain AMPs at nanomolar concentration to pHEMA is an effective option to develop a stable antimicrobial surface.

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Cited by 6 publications

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Detection of foodborne pathogens by qPCR: A practical approach for food industry applications

Detection of foodborne pathogens by qPCR: A practical approach for food industry applications

Biological control of pre-harvest aflatoxin contamination in groundnut (Arachis hypogaeaL.) withBacillus subtilisG1

Antimicrobial activity of four cationic peptides immobilised to poly-hydroxyethylmethacrylate

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