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doi:10.1002/cyto.v87.8

Cited by 6 publications

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“…26 When applied to PA signals containing ultrahigh frequencies (UHFs), such techniques enable the extraction of 3-D morphological information of optically absorbing cells such as RBCs and melanocytes. [27][28][29] Combining PA spectral analysis techniques with an equivalent US-based technique, capable of determining the size of liquid droplets from features in the power spectrum of backscattered US waves, 30,31 we have previously been able to assess the N:C ratio in cultured cancer cells. 32,33 However, our previous work only demonstrated the use of these techniques for a single cell line with a small sample size and used published N:C ratio values as a preliminary means of technique validation.…”

Section: Introductionmentioning

confidence: 99%

Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study

2019

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Section: Introductionmentioning

confidence: 99%

Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study

2019

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Section: Introductionmentioning

confidence: 99%

Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study

Moore¹,

Sebastian²,

Kolios³

2022

Preprint

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<p>While the nucleus-to-cytoplasmic (N:C) ratio has traditionally been used for assessing cell malignancy, most N:C measurement techniques are time-consuming and performed on thin histological sections, which prohibit assessment of three-dimensional cell structure. A combined ultrahigh frequency ultrasound (US) and photoacoustic (PA) technique was used to assess the size and N:C ratio of cultured cancer cells in three dimensions (3D). The diameters of the cells and their stained nuclei were obtained by fitting the power spectrum of backscattered US pulses and emitted PA waves, respectively, to well-established theoretical models. For comparison, an imaging flow cytometer (IFC) was also used to determine the two-dimensional cell and nucleus sizes from large cell populations using brightfield and fluorescence images, respectively. An N:C ratio was calculated for each cell using the quotient of the measured nucleus diameter and the total cell diameter. The mean N:C ratios calculated using the sound-based approach were 0.68, 0.66, and 0.54 for MCF-7, PC-3, and MDA-MB-231 cells, respectively, and were in good agreement with the corresponding values of 0.68, 0.67, and 0.68 obtained using the IFC. The combined US and PA technique, which assesses cellular N:C ratio in 3D, has potential applications in the detection of circulating tumor cells in liquid biopsies. </p>

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“…12 However, PAM's axial resolution is limited because conventional piezoelectric transducers have difficulty in detecting GHz frequency acoustic signals, which encode higher resolution information. Whereas progress has been made in the development of high-frequency transducers, 21,22 transducers that can sensitively detect signals in the range of several GHzs are not commonly available commercially. Among high bandwidth transducers that are available, high acoustic frequency attenuation due to the large distance between the source and the detector remains a problem.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale photoacoustic tomography for label-free super-resolution imaging: simulation study

et al. 2018

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Resolutions higher than the optical diffraction limit are often desired in the context of cellular imaging and the study of disease progression at the cellular level. However, three-dimensional super-resolution imaging without reliance on exogenous contrast agents has so far not been achieved. We present nanoscale photoacoustic tomography (nPAT), an imaging modality based on the photoacoustic effect. nPAT can achieve a dramatic improvement in the axial resolution of the photoacoustic imaging. We derive the theoretical resolution and sensitivity of nPAT and demonstrate that nPAT can achieve a maximum axial resolution of 9.2 nm. We also demonstrate that nPAT can theoretically detect smaller numbers of molecules (∼273) than conventional photoacoustic microscopy due to its ability to detect acoustic signals very close to the photoacoustic source. We simulate nPAT imaging of malaria-infected red blood cells (RBCs) using digital phantoms generated from real biological samples, showing nPAT imaging of the RBC at different stages of infection. These simulations show the potential of nPAT to nondestructively image RBCs at the nanometer resolutions for in vivo samples without the use of exogenous contrast agents. Simulations of nPAT-enabled functional imaging show that nPAT can yield insight into malarial metabolism and biocrystallization processes. We believe that the experimental realization of nPAT has important applications in biomedicine.

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Cited by 6 publications

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Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study

Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study

Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study

Nanoscale photoacoustic tomography for label-free super-resolution imaging: simulation study

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